Blotting Northern Methods
Mostrando 1-12 de 23 artigos, teses e dissertações.
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1. NUDEL-OLIGOPEPTIDASE: ANÁLISE DA DISTRIBUIÇÃO DO RNAm NO SNC DE RATOS NEONATOS E ADULTOS E CARACTERIZAÇÃO DO RESÍDUO CRÍTICO PARA A ATIVIDADE CATALÍTICA. / NUDEL-oligopeptidase: analysis of mRNA distribution in the central nervous system (CNS) of newborn and adult rats, and characterization of the amino acid residue critical for the catalytic activity.
Thirty years ago the first endooligopeptidases were described, and named Endo A (EOPA) and Endo B. EOPA was later characterized as a thiol-activated peptidase showing specificity for substrates comprising 7 to 13 amino acid residues. In 2000, studying the molecular mechanisms of the brain disorder lissencephaly, three independent groups isolated a protein na
Publicado em: 2006
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2. "Determinação do perfil de expressão dos RNAs mensageiros da família das Smads e dos componentes do complexo AP-1 em carcinoma de célula escamosa de cabeça e pescoço" / Smads and AP-1 messenger RNA expression pattern in Head and Neck Squamous Cell Carcinoma
Smad and AP1 messenger RNA expression may underlie disruptions affecting TGFb signaling in head and neck squamous cell carcinoma (HNSCC). Analysis of Smads1-8 mRNA expression by RPA has shown Smad expression is globally increased in tumor as compared to adjacent normal tissue. Kaplan Meier survival curves and multivariate analysis revealed that Smad6 positiv
Publicado em: 2005
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3. Methods for the Detection of D-Amino-Acid Oxidase
Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
Biological Procedures Online.
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4. Verification of differential gene transcription using virtual northern blotting
We introduce here an alternative to conventional northern blotting that requires only minute amounts of RNA. This has been achieved by modification of methods currently used for the mapping of mRNA 5'-terminal ends. The terminal desoxynucleotidyl transferase-mediated G-tailing, cap finder, ligation-anchored and RNA ligase-mediated approachesfollowed by polym
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5. Transcriptional Organization of the Erythromycin Biosynthetic Gene Cluster of Saccharopolyspora erythraea
The transcriptional organization of the erythromycin biosynthetic gene (ery) cluster of Saccharopolyspora erythraea has been examined by a variety of methods, including S1 nuclease protection assays, Northern blotting, Western blotting, and bioconversion analysis of erythromycin intermediates. The analysis was facilitated by the construction of novel mutants
American Society for Microbiology.
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6. Recombinant equine interleukin-1β induces putative mediators of articular cartilage degradation in equine chondrocytes
Interleukin-1 is considered a central mediator of cartilage loss in osteoarthritis in several species, however an equine recombinant form of this cytokine is not readily available for in vitro use in equine osteoarthritis research. Equine recombinant interleukin-1β was cloned and expressed and its effects on the expression and activity of selected chondrocy
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7. rRNA Stability in Heat-Killed and UV-Irradiated Enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7†
Differentiation of viable cells from nonviable cells is of considerable importance in the development of methods to detect foodborne pathogens. To study the suitability of 16S rRNA as an indicator of cell viability in nucleic acid-based detection assays, we examined rRNA stability in two representative foodborne pathogens, Escherichia coli O157:H7 and entero
American Society for Microbiology.
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8. Effect of Mycobacterium tuberculosis and its components on macrophages and the release of matrix metalloproteinases.
BACKGROUND: Pulmonary tuberculosis is associated with caseating necrosis, parenchymal lung destruction, and cavity formation. It was hypothesised that tuberculous lung destruction is mediated, at least in part, by the participation of matrix metalloproteinases released by mononuclear phagocytes. METHODS: Cells of the myelomonocytic leukaemia cell line THP-1
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9. Multiplex messenger assay: simultaneous, quantitative measurement of expression of many genes in the context of T cell activation.
The hybridization signature approach, using colony filters and labeled complex probes, can provide high throughput measurement of gene activity. We describe here the implementation of this method to follow the expression levels of 47 genes in resting and activated T cells, as well as in epithelial cells. Using 4-fold spotting of colonies, imaging plate detec
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10. In vivo splicing of the premRNAs from early region 3 of adenovirus-2: the products of cleavage at the 5' splice site of the common intron.
The nuclear transcripts of the early region 3 from adenovirus-2 were studied for the presence of the cleavage products of premRNA at the 5' splice site of the first intervening sequence. Two molecules, free exon 1 and intron-exon 2-poly(A) were characterized by complementary methods including Northern blotting, RNase and S1 nuclease mapping, hybrid-selection
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11. The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates.
Previous work has shown that RNase E-mediated cleavage of RNAI, an antisense repressor of the replication of ColE1-type plasmids, relieves repression in vivo by endonucleolytically converting RNAI to a rapidly decaying product. We report that mutations in the Escherichia coli pcnB gene result in a 10-fold prolongation of the half-life of RNAI decay intermedi
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12. Abnormal expression and mutation of p53 in cervical cancer--a study at protein, RNA and DNA levels.
OBJECTIVES: The objectives of this study are to document the status of p53 expression and mutation in cervical cancer at protein, RNA and DNA levels and to relate this to the presence of HPV. MATERIALS AND METHODS: Biopsy specimens from one hundred and three squamous cell carcinoma of the cervix and histologically normal ectocervix were analysed. Fresh tissu