Bovine Mtdna
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Mitochondrial and Y chromosome genetic diversity in the Portuguese Lidia bovine breed
ABSTRACT A total of 80 samples were collected to analyze the mitochondrial DNA (mtDNA) and Y chromosome genetic diversity of two main Portuguese Lidia bovine populations and clarify their genetic relationships with Spanish Lidia lineages. A 521-bp D-loop fragment was sequenced in 40 animals belonging to four herds (three Brava dos Açores and one Casta Portu
R. Bras. Zootec.. Publicado em: 2017-02
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2. Single nucleotide polymorphisms from cytochrome b gene as a useful protocol in forensic genetics against the illegal hunting of manatees: Trichechus manatus, Trichechus inunguis, Trichechus senegalensis, and Dugong dugon (Eutheria: Sirenia)
The identification of mitochondrial DNA polymorphisms is one of the most efficient methods for species differentiation. Genotyping of molecular markers using PCR/RFLP is a reliable, sensitive and inexpensive method for the detection of species specific mutations. The major causes of decline in Sirenia populations are accidental and intentional catches, colli
Zoologia (Curitiba). Publicado em: 2011-02
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3. Characterization of mitochondrial genotypes in the foundation herd of the Canchim beef cattle breed
The Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis. The genotypic origin (Bos tauru
Genetics and Molecular Research. Publicado em: 2011
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4. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offsp
Biology of Reproduction. Publicado em: 2011
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5. Mitochondrial control region genetic diversity and maternal ancestry of a Brangus-Ibage cattle populations
The genetic diversity of 277 nucleotides in the mitochondrial DNA control region (nt 15,964 to 16,240 in reference sequence) was analyzed in crossbreed beef cattle (Brangus-Ibage, 5/8 Bos primigenius taurus x 3/8 Bos primigenius indicus) as well as in some Nellore samples (B. p. indicus). Fifty-seven mutations were found in Brangus-Ibage comprising 18 haplot
Publicado em: 2010
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6. Protein binding to a single termination-associated sequence in the mitochondrial DNA D-loop region.
A methylation protection assay was used in a novel manner to demonstrate a specific bovine protein-mitochondrial DNA (mtDNA) interaction within the organelle (in organello). The protected domain, located near the D-loop 3' end, encompasses a conserved termination-associated sequence (TAS) element which is thought to be involved in the regulation of mtDNA syn
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7. Association of mitochondrial antioxidant enzymes with mitochondrial DNA as integral nucleoid constituents
Mitochondrial DNA (mtDNA) is organized in protein-DNA macrocomplexes called nucleoids. Average nucleoids contain 2–8 mtDNA molecules, which are organized by the histone-like mitochondrial transcription factor A. Besides well-characterized constituents, such as single-stranded binding protein or polymerase γ (Polγ), various other proteins with ill-defined
The Federation of American Societies for Experimental Biology.
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8. Replacement of Bovine Mitochondrial DNA by a Sequence Variant within One Generation
Inheritance of mitochondrial DNA (mtDNA) in Holstein cattle was characterized by pedigree analysis of nucleotide sequence variation. mtDNA was purified from leukocytes of 174 individuals representing 35 independent maternal lineages, and analyzed for nucleotide sequence variation by characterization of restriction fragment length polymorphism and direct sequ
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9. DNA helicase from mammalian mitochondria.
In spite of the fact that a DNA helicase is clearly required for the predominantly leading-strand synthesis occurring during mammalian mtDNA replication, no such activity has heretofore been identified. We report the characterization of a mammalian mitochondrial DNA helicase isolated from bovine brain tissue. The sucrose gradient-purified mitochondria in whi
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10. Novel features of animal mtDNA evolution as shown by sequences of two rat cytochrome oxidase subunit II genes.
The sequence of the region of the mitochondrial genome that encodes cytochrome oxidase subunit II (COII) has been determined for each of two closely related rat species, Rattus norvegicus and R. rattus. Comparison of the two sequences shows that 94.4% of the nucleotide substitutions are silent. The occurrence of this high proportion of silent substitutions l
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11. Molecular structures of mitochondrial-DNA-like sequences in human nuclear DNA.
Two lambda phage clones carrying mitochondrial-DNA-like (mtDNA-like) sequences isolated from a human gene library were named Lm E-1 and Lm C-2, and their DNA structures were characterized. Lm E-1 contains about 0.4 kb DNA homologous to the 5' portion of the mitochondrial 16S ribosomal RNA (rRNA) gene and Lm C-2, a 1.6 kb DNA homologous to the 3' portion of t
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12. Five TGA “stop” codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II
A mitochondrial mutation that genetically maps in the middle of the gene coding cytochrome c oxidase subunit II has been found to be a single-base-pair deletion. Three independently isolated spontaneous revertants of this mutant have different single-base-pair insertions within 15 nucleotides of the mutation. These findings clearly identify the location of t