Candida Isolation Purification
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. Identification, isolation and optimization of antifungal metabolites from the Streptomyces Malachitofuscus ctf9
An indigenous Streptomyces isolate CTF9, exhibiting promising antifungal activity against Mucor miehei and Candida albicans in pre-screening studies, was investigated by cultivation in a 50-L fermenter and by subsequent isolation, purification, and structure elucidation of the active metabolites. Based on the morphological, biochemical, and physiological cha
Brazilian Journal of Microbiology. Publicado em: 2011-06
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2. Seleção de leveduras para bioconversão de D-xilose em xilitol / Yeast selection for bioconversion of D-xylose to xilitol
Microbial species, particularly yeast, are of great importance for the production of xylitol. The xylitol production involves complicated metabolic regulation, including the transport of D-xylose, production of key enzymes and cofactor regeneration. Thus, screening of microorganisms that consume D-xylose naturally becomes a viable and effective way to obtain
Publicado em: 2010
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3. Colonização por Candida em indivíduos com candidemia / Candida colonization in individuals with candidemia
In the last two decades, Candida spp. have emerged as important nosocomial pathogens in the world and in Brazil. The identification of the source of infection is important in approaching prevention and control strategies. Strategies for the prevention of endogenous candidiasis may focus, to a certain extent, on methods for reducing mucosal colonization, for
Publicado em: 2008
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4. Identification of Elongation Factor 2 as the Essential Protein Targeted by Sordarins in Candida albicans
The target for sordarins in Candida albicans has been elucidated. Kinetic experiments of sordarin inhibition as well as displacement experiments showed that the formation of a sordarin-target complex follows a reversible mechanism. Binding of tritiated drug to the target is enhanced in the presence of ribosomes. Isolation of the target by classical protein p
American Society for Microbiology.
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5. Comparative production and rapid purification of Candida acid proteinase from protein-supplemented cultures.
Six Candida spp. that were previously characterized for cutaneous pathogenicity were assessed for Candida acid proteinase (CAP) production in albumin-supplemented, nitrogen-restricted media. C. albicans CAP production was compared in media supplemented with albumin, casein, collagen, hemoglobin, or keratin and in TC medium 199. C. albicans, C. stellatoidea,
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6. Isolation and characteristics of collagenolytic enzyme produced by Candida albicans.
In media containing collagen as the nitrogen source, the pathogenic yeast Candida albicans secreted a collagenolytic enzyme. Purification of the enzyme from a culture filtrate was achieved by DEAE-Sephacel chromatography at pH 6.7. The molecular weight was found to be 46,000 by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the isoelectri
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7. Analysis of mannans of two relatively avirulent mutant strains of Candida albicans.
We previously reported the isolation of two cerulenin-resistant mutant strains of Candida albicans 4918 that differ in adherence properties and are less virulent than the parental strain. In addition, biochemical characterization demonstrated significant differences in both protein and polysaccharide composition of cell wall material between the mutant and w
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8. Isolation and partial characterization of glycolipid fractions from Mycobacterium avium serovar 2 (Mycobacterium paratuberculosis 18) that inhibit activated macrophages.
Glycolipid fractions from Mycobacterium avium serovar 2 (Mycobacterium paratuberculosis 18) inhibited the killing of Candida albicans by activated bovine peripheral-blood-derived macrophages. Fractions were derived by using the matrix solid-phase dispersion technique, which is a new method of simultaneous lysis and partial fractionation of components of bact
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9. Isolation and preliminary characterization of the 14- to 18-kilodalton Candida albicans antigen as a phospholipomannan containing beta-1,2-linked oligomannosides.
Western blot (immunoblot) analysis of Candida albicans germ tube extracts has demonstrated the probable presence of beta-1,2-linked oligomannosides acting as epitopes distributed over a 14- to 18-kDa antigen unreactive to concanavalin A. These conclusions about the existence of these non-mannan-associated oligomannoside species were reinforced in the present
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10. Isolation and biochemical characterization of the iC3b receptor of Candida albicans.
In an effort to identify the protein structure on Candida albicans, pseudohyphal forms which had been shown earlier to bind human iC3b, a protein of about 42 kDa (p42), were obtained from lysates of pseudohyphal forms by absorption with C3(H2O)-Sepharose. An antiserum raised in rabbits against this protein effectively inhibited adherence of sheep erythrocyte
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11. Isolation, purification, and radiolabeling of a novel 120-kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients.
No well-defined Blastomyces-specific antigens are currently available. We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to identify immunologically active molecules in the cell wall of B. dermatitidis. A major immunoreactive 120-kD protein (WI-1) was present in all five strains studied and comprised 5% of the protein in th