Capillary Gel Electrophoresis
Mostrando 1-12 de 62 artigos, teses e dissertações.
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1. Retraction notice for: “Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients” [Braz J Med Biol Res (2018) 51(7): e6830]
Due to its various structures in bio-compounds, snake venom is the indisputable result of evolutionary stages of molecules with an increasingly complex structure, high specificity, and of great importance for medicine because of their potential. The present study proposed an underpinning examination of venom composition from nine species of venomous snakes u
Braz J Med Biol Res. Publicado em: 2020-01
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2. Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents
Due to its various structures in bio-compounds, snake venom is the indisputable result of evolutionary stages of molecules with an increasingly complex structure, high specificity, and of great importance for medicine because of their potential. The present study proposed an underpinning examination of venom composition from nine species of venomous snakes u
Braz J Med Biol Res. Publicado em: 2020-01
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3. FUNDAMENTOS DE ELETROFORESE CAPILAR: UMA ABORDAGEM POR ANIMAÇÕES
Among the analytical separation techniques, capillary electrophoresis (CE) has attracted attention in both academic and industrial fields, mainly owing to its efficiency, short analysis times, low reagent consumption and residue generation. This is also the case in Chemistry courses, books and papers, demonstrating CE´s well-established status. However,
Quím. Nova. Publicado em: 2015-06
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4. Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity
Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs. Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims. Methods In the present study, the peptide profile of the v
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 02/12/2014
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5. A Boophilus microplus vitellin-degrading cysteine endopeptidase
Here we describe the purification and characterization of a vitellin (VT) degrading cysteine endopeptidase (VTDCE) from eggs of the hard tick Boophilus microplus. A homogeneous enzyme preparation was obtained by chromatographic fractionation on ion-exchange and gel filtration columns and an autolysis step. This step consisted of incubation of a semipurified
Publicado em: 2011
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6. Neurotoxic activity and ultrastructural changes in muscles caused by the brown widow spider Latrodectus geometricus venom
Brown widow spider (Latrodectus geometricus) venom (BrWSV) produces few local lesions and intense systemic reactions such as cramps, harsh muscle pains, nausea, vomiting and hypertension. Approximately 16 protein bands under reducing conditions and ~ 14 bands under non-reducing conditions on a 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis w
Revista do Instituto de Medicina Tropical de São Paulo. Publicado em: 2009-04
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7. Desenvolvimento de metodologia para separação de carboidratos predominantes em alimentos por eletroforese capilar / Method development for the separation of carbohydrates predominant in foods by capillary electrophoresis
Os carboidratos são compostos muito abundantes na natureza. Estão presentes em todas as etapas evolutivas, tanto em vegetais como animais. Quaisquer reações, de anabolismo ou catabolismo envolvem, de alguma forma, algum carboidrato. Na indústria alimentícia são responsáveis por diversos fenômenos que promovem efeitos químicos, físicos e sensoriais
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 20/08/2008
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8. Cafeína: revisão sobre métodos de análise
Gravimetric and Bailey-Andrew methods are tedious and provide inflated results. Spectrofotometry is adequate for caffeine analysis but is lengthy. Gas chromatography also is applied to the caffeine analysis but derivatization is needed. High performance liquid chromatography with ultraviolet detection (HPLC-UV) and reversed phase is simple and rapid for xant
Química Nova. Publicado em: 2007-02
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9. Capillary electrophoresis-based biomolecular recognition studies / Estudos de reconhecimento biomolecular por eletroforese capilar
This Thesis concerns on the development of capillary electrophoresis-based bioanalytical methods for two distinct applications: DNA-protein binding analysis and monitoring of enzyme-catalyzed GTP hydrolysis. In the first chapter, the feasibility of on-chip electrophoretic mobility-shift assays (EMSA) is investigated. Purified transcription factors c-Jun(AP1)
Publicado em: 2005
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10. Métodos para análise de ácido clorogênico
This paper describes the analytical methods for determination of total chlorogenic acid (CGA) and their individual isomers. Spectrofotometric methods are adequate for total CGA analysis in green coffee but they can provide inflated results for coffee products. High pressure liquid chromatography (HPLC) with gel permeation column and ultraviolet (UV) monitori
Química Nova. Publicado em: 2004-08
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11. Capillary gel electrophoresis for rapid, high resolution DNA sequencing.
Capillary gel electrophoresis has been demonstrated for the separation and detection of DNA sequencing samples. Enzymatic dideoxy nucleotide chain termination was employed, using fluorescently tagged oligonucleotide primers and laser based on-column detection (limit of detection is 6,000 molecules per peak). Capillary gel separations were shown to be three t
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12. Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the