Cdna And Prostate Tumors
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. MÃtodo alternativo para obtenÃÃo de Ãcido nuclÃicos a partir de tecidos prostÃticos parafinizados
O objetivo deste estudo foi otimizar um protocolo de extraÃÃo do RNA de fragmentos de tecidos tumorais de prÃstatas, contendo hiperplasia e adenocarcinoma, fixados em formol e conservados em blocos de parafina. Em seguida, a qualidade do RNA extraÃdo foi avaliada mediante a amplificaÃÃo por PCR do exon 4 do gene TP53, onde se observa um polimorfismo no
Publicado em: 2009
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2. VariaÃÃes transcricionais dos genes AR, SRD5A2, KLK2, PCA3, KLK3 e PSMA e implicaÃÃes no diagnÃstico molecular do cÃncer de prÃstata
CHAPTER I - Prostate cancer is a common disease in the world and in some countries it is one of the main causes of male population mortality. Some molecular markers have been associated with prostate carcinogenesis. To observe changes in transcript levels of the AR, SRD5A2, KLK2, PSMA and PCA3 genes, the mRNA was analyzed in tissues with prostatic adenocarci
Publicado em: 2007
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3. Gene expression profiling identifies clinically relevant subtypes of prostate cancer
Prostate cancer, a leading cause of cancer death, displays a broad range of clinical behavior from relatively indolent to aggressive metastatic disease. To explore potential molecular variation underlying this clinical heterogeneity, we profiled gene expression in 62 primary prostate tumors, as well as 41 normal prostate specimens and nine lymph node metasta
National Academy of Sciences.
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4. STEAP: A prostate-specific cell-surface antigen highly expressed in human prostate tumors
In search of novel genes expressed in metastatic prostate cancer, we subtracted cDNA isolated from benign prostatic hypertrophic tissue from cDNA isolated from a prostate cancer xenograft model that mimics advanced disease. One novel gene that is highly expressed in advanced prostate cancer encodes a 339-amino acid protein with six potential membrane-spannin
The National Academy of Sciences.
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5. Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis
A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot bl
National Academy of Sciences.
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6. PAGE-1, an X chromosome-linked GAGE-like gene that is expressed in normal and neoplastic prostate, testis, and uterus
We have used a combination of computerized database mining and experimental expression analyses to identify a gene that is preferentially expressed in normal male and female reproductive tissues, prostate, testis, fallopian tube, uterus, and placenta, as well as in prostate cancer, testicular cancer, and uterine cancer. This gene is located on the human X ch
National Academy of Sciences.
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7. The FEZ1 gene at chromosome 8p22 encodes a leucine-zipper protein, and its expression is altered in multiple human tumors
Alterations of human chromosome 8p occur frequently in many tumors. We identified a 1.5-Mb common region of allelic loss on 8p22 by allelotype analysis. cDNA selection allowed isolation of several genes, including FEZ1. The predicted Fez1 protein contained a leucine-zipper region with similarity to the DNA-binding domain of the cAMP-responsive activating-tra
The National Academy of Sciences.
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8. Identification of the human prostatic carcinoma oncogene PTI-1 by rapid expression cloning and differential RNA display.
Elucidating the relevant genomic changes mediating development and evolution of prostate cancer is paramount for effective diagnosis and therapy. A putative dominant-acting nude mouse prostatic carcinoma tumor-inducing gene, PTI-1, has been cloned that is expressed in patient-derived human prostatic carcinomas but not in benign prostatic hypertrophy or norma
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9. Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor: In vivo expression and antitumor activity
Neuropilin-1 (NRP1) is a 130-kDa transmembrane receptor for semaphorins, mediators of neuronal guidance, and for vascular endothelial growth factor 165 (VEGF165), an angiogenesis factor. A 2.2-kb truncated NRP1 cDNA was cloned that encodes a 644-aa soluble NRP1 (sNRP1) isoform containing just the a/CUB and b/coagulation factor homology extracellular dom
The National Academy of Sciences.
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10. Reverse biochemistry: Use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue
Serine proteases of the chymotrypsin fold are of great interest because they provide detailed understanding of their enzymatic properties and their proposed role in a number of physiological and pathological processes. We have been developing the macromolecular inhibitor ecotin to be a “fold-specific” inhibitor that is selective for members of the c
The National Academy of Sciences.
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11. Prevention of metastasis by inhibition of the urokinase receptor.
The plasminogen activator urokinase (u-PA) mediates proteolysis by a variety of human tumor cells. Competitive displacement of u-PA from cellular binding sites results in decreased proteolysis in vitro, suggesting that the cell surface is the preferred site for u-PA-mediated protein degradation. We studied the effect of u-PA receptor blockade on the metastat