Chaperone Molecular
Mostrando 1-12 de 403 artigos, teses e dissertações.
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1. Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp (Penaeus monodon)
Abstract P53 And DNA Damage-Regulated Gene 1 (PDRG1) is a novel gene which plays an important role in chaperone-mediated protein folding. In the present study, the full-length complementary DNA (cDNA) sequence of the PDRG1 gene from Penaeus monodon (PmPDRG1) was cloned by the rapid amplification of cDNA ends (RACE) method. The cDNA of PmPDRG1 spans 1,613 bp,
Genet. Mol. Biol.. Publicado em: 02/03/2017
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2. HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics
The 70 kDa heat shock protein (HSP70) is a molecular chaperone that assists the parasite Leishmania in returning to homeostasis after being subjected to different types of stress during its life cycle. In the present study, we evaluated the effects of HSP70 transfection of L. amazonensis promastigotes (pTEX-HSP70) in terms of morphology, resistance, infectiv
Mem. Inst. Oswaldo Cruz. Publicado em: 10/06/2016
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3. Heat Shock Protein Hspa5 Interacts with and Protects Tyrosinase Activity
In this study, heat shock protein, Hspa5 was cloned, expressed and purified subsequently confirmed that it interacted with the tyrosinase (TYR) in vitro. Then, using the crystal structure of the homologous protein from the bacteria as a template, a homology model of human TYR was constructed. This model was further applied to investigate the molecular dockin
Braz. arch. biol. technol.. Publicado em: 2015-08
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4. Estudos estruturais e funcionais da Hsp90 de Leishmania braziliensis e suas co-chaperonas p23 / Structural and functional studies of Leishmania braziliensis Hsp90 and its p23 co-chaperones
Molecular chaperones are proteins involved in proper folding of other proteins, and others important cellular functions, why they have been targeted for combating various diseases. The Hsp90 (82-96 kDa) are ubiquitous chaperones that interact with a wide range of client proteins. They are formed by three domains: N-terminal, central or middle (M), and C-term
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/06/2012
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5. Estudos iniciais de ineraçãos da HSP90 através da caracterização funcioanl de um transgênico e biofísica de uma co-chaperona / Insights on Hsp90 chaperone interactions using transgenic and biophysical approaches
Chaperonas moleculares (Heat Shock proteins - HSPs) são componentes chave do sistema de controle de qualidade de proteínas (PQC - Protein Quality Control), que é essencial para a vida, sendo responsável por manter a homeostase proteica e a adequada função de diversas vias. Problemas no processo de enovelamento estão relacionados a doenças degenerativ
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/02/2012
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6. Clonagem, expressão heteróloga e caracterização da proteína de escolta da Hsp70 de Leishmania braziliensis / Cloning, heterologous expression and characterization of protein Hsp70 escort of Leishmania braziliensis
A Leishmaniose é uma doença infecciosa causada por protozoários flagelados do gênero Leishmania. Os parasitas como a Leishmania braziliensis, sofrem várias mudanças morfológicas durante seu ciclo de vida, incluindo a troca de organismo hospedeiro. Durante essas mudanças, proteínas de choque térmico ou chaperones moleculares, como, por exemplo, a Hs
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/08/2011
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7. Caracterização e interação do domínio C-terminal da chaperona Hsp90 humana e das co-chaperonas Tom 70 e Hop / Characterization and interaction of the C-terminal domain of the human chaperone Hsp90 and co-chaperones Tom 70 and Hop
A função biológica das proteínas está relacionada à sua estrutura tridimensional adquirida pelo processo de enovelamento protéico. Neste contexto, proteínas denominadas, genericamente, de chaperonas moleculares exercem papel fundamental atuando no auxílio do enovelamento correto, no reenovelamento e na dissociação de agregados protéicos. A Hsp90
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/08/2011
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8. Caracterização de determinantes genéticos envolvidos na qualidade industrial e nutricional do fruto de tomate / Characterization of genetic determinants involved in industrial and nutritional quality of tomato fruit
This thesis addresses tomato fruit metabolism analysis by using three complementary approaches: i) the identification of candidate genes co-located with QTL previously described from metabolic profiles of S. lycopersicum (cv. M82) and S. pennellii introgressed lines, associated with industrial and nutritional quality traits; ii) the structural analyses of th
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/08/2011
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9. A expressão deficiente das chaperonas GRP78 e GRP94 conecta a sinalização de TLR4 com o estresse de retículo endoplasmático / Chaperone insuficiency of GRP78 and GRP94 links TLR4 signaling to endoplasmic reticulum stress
TLR4 activation and the induction of endoplasmic reticulum stress (ERS) are two of the most important mechanisms connecting excessive fat with insulin resistance. Recently, it was shown that activation of TLR4 can, per se, induce ERS, suggesting that TLR4 is a primary event in the induction of the cellular stress that contributes to increased inflammatory ge
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/04/2011
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10. Development of an in vitro model of expanded ataxin-3 cytotoxic effects and evaluation of different therapeutic strategies to control of these effects / Desenvolvimento de um modelo in vitro dos efeitos citotóxicos da ataxina-3 expandida e avaliação de diferentes estratégias terapêuticas para o controle desses efeitos
Spinocerebellar ataxia-3 (SCA3), also known as Machado-Joseph disease (MJD), belongs to a group of neurodegenerative disorders caused by expansion of a polyglutamine stretch, called polyglutamine diseases. MJD is the most frequent inherited autosomal dominant ataxia in many countries. Clinical manifestations are varied, including abnormal motor coordination
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/02/2010
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11. Analise da expressão de chaperonas moleculares em plantas e clonagem, purificação e caracterização inicial das proteinas Hsp100 e Hsp90 de cana-de-açucar / Expression analysis of plant molecular chaperones and cloning, purification and primary charaterization of the proteins Hsp 100 and Hsp90 from sugarcane
Proteins are macromolecules that are vital to the functioning cell, participating in most of the biological reactions as well as structural components. To perform its function, a protein need to achieve its native structure through a process called protein folding. In this context, the molecular chaperone proteins are able to assist in the folding of other p
Publicado em: 2009
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12. Structural studies of the proteins Q4DV70 from Trypanosoma cruzi and mesothelin from Homo sapiens / Estudos estruturais das proteinas Q4DV70 de Trypanosoma cruzi e mesotelina de Homo sapiens
In this work we carried out studies with two proteins, Q4DV70 from Trypanosoma cruzi and mesothelin from Homo sapiens. The aim was to help the understanding of the function of these proteins which possibly are important to Chagas disease and cancer, respectively. Q4DV70 protein was annotated in T. cruzi genome as a conserved hypothetical protein. In our stud
Publicado em: 2009