Chitosanases
Mostrando 1-7 de 7 artigos, teses e dissertações.
-
1. Produção de enzimas quitosanolíticas utilizando Paenibacillus ehimensis e Paenibacillus chitinolyticus para obtenção de quitoologossacarídeos
A obtenção de oligossacarídeos a partir da quitosana tem sido alvo de diversas pesquisas na área farmacêutica, bioquímica, alimentar e médica, devido às propriedades funcionais destes compostos. Este trabalho teve o objetivo de potencializar a produção de quitooligossacarídeos (QOS), através da otimização da produção e caracterização de enz
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/03/2011
-
2. Produção e caracterização de quitooligossacarídeos produzidos pelo fungo Metarhizium anisopliae e avaliação da citotoxicidade em células tumorais
A quitosana é um polímero natural, biodegradável, não tóxico e de alta massa molecular obtido a partir de animais marinhos, insetos e microrganismos. Os oligômeros de glicosamina (GlcN) e N-acetilglicosamina (GlcNAc) têm atividades biológicas interessantes, incluindo efeitos antitumorais, atividade antimicrobiana, antioxidante entre outras. A alterna
Publicado em: 2010
-
3. Estudo do mecanismo de produção de oligossacarídeos com atividades nutracêuticas a partir da quitosana por hidrólise enzimática com processo fermentativo simultâneo
The obtaining of the oligosaccharides from chitosanase, has showed interest of the pharmaceutical area in the last years due their countless functional properties. Although, the great challenge founded out is how to keep a constant and efficient production. The alternative proposed by this present work was to study the viability to develop an integrated tech
Publicado em: 2008
-
4. Recuperação e purificação de enzimas usando adsorção em leito expandido
This work deals with the application of Expanded Bed Adsorption (EBA) to recovery and purify enzymes. Model proteins, lisozyme and bovine serum albumin (BSA), as well as some enzymes, glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from baker s yeast, xylanase (from a commercial extract) and chitosanase from Bacil/us cereus were used to study the EBA backgr
Publicado em: 2001
-
5. Purification and Characterization of Three Chitosanase Activities from Bacillus megaterium P1
Bacillus megaterium P1, a bacterial strain capable of hydrolyzing chitosan, was isolated from soil samples. Chitosan-degrading activity was induced by chitosan but not by its constituent d-glucosamine. Extracellular secretion of chitosanase reached levels corresponding to 1 U/ml under optimal conditions. Three chitosan-degrading proteins (chitosanases A, B,
-
6. Purification, Characterization, and Gene Analysis of a Chitosanase (ChoA) from Matsuebacter chitosanotabidus 3001
The extracellular chitosanase (34,000 Mr) produced by a novel gram-negative bacterium Matsuebacter chitosanotabidus 3001 was purified. The optimal pH of this chitosanase was 4.0, and the optimal temperature was between 30 and 40°C. The purified chitosanase was most active on 90% deacetylated colloidal chitosan and glycol chitosan, both of which were hydroly
American Society for Microbiology.
-
7. Barley pathogenesis-related proteins with fungal cell wall lytic activity inhibit the growth of yeasts.
Proteins from intercellular fluid extracts of chemically stressed barley (Hordeum vulgare L.) leaves were separated by native polyacrylamide gel electrophoresis at alkaline or acid pH. Polyacrylamide gels contained Saccharomyces cerevisiae (bakers' yeast) or Schizosaccharomyces pombe (fission yeast) crude cell walls for assaying yeast wall lysis. In parallel