Colicin
Mostrando 1-12 de 503 artigos, teses e dissertações.
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1. Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
Most of the knowledge of the virulence determinants of extraintestinal pathogenicEscherichia coli (ExPEC) comes from studies with human strains causing urinary tract infections and neonatal meningitis and animal strains causing avian colibacillosis. In this research, we analyzed the phylogenetic background, the presence of 20 ExPEC virulence factors, and the
Braz J Med Biol Res. Publicado em: 15/10/2013
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2. Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea
Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be t
Braz. J. Microbiol.. Publicado em: 2013-09
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3. Serogroups and virulence genotypes of Escherichia coli isolated from patients with sepsis
Sixty strains of Escherichia coli, isolated by hemoculture, from septicemic Brazilian patients were evaluated to determine their serogroup and invasivity to Vero cells. All 60 patients died within 2 days of hospitalization. Furthermore, the molecular study of the following extraintestinal pathogenic E. coli-associated virulence factor (VF) genes was performe
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-10
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4. O stimulon de ferro em Xylella fastidiosa / The iron stimulon of Xylella fastidiosa
Xylella fastidiosa é o agente etiológico de diversas doenças em plantas, incluindo a Clorose Variegada dos Citros (CVC), uma séria ameaça à indústria citrícola. Os níveis de transcritos sob diferentes disponibilidades de ferro foram medidos com microarranjos de DNA representando 2608 (91,6%) sequências codificadoras (CDS) da cepa 9a5c de X. fastidi
Publicado em: 2007
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5. Biologicals and moleculars methods in the study of virulence factors in Eschericia coli strains isolated by blood culture of patients with septicaemia / Metodos biologicos e moleculares no estudo de fatores de virulencia em amostras de Eschericia coli isoladas por hemocultura de pacientes com septicemia
The pathogenicity of Escherichia coli in extraintestinal infections (ExPEC) depends to virulence properties bacterial. This, genetically, spread by several pathogenicity associated islands ? genic blocks contiguous ? responsible to express factors that allow to bacteria colonize, survive and thus, hurt the host integrity. Seventh tree strains of E. coli isol
Publicado em: 2006
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6. Interaction of 125I-Labeled Colicin E1 with Escherichia coli
Colicin E1 protein was labeled with 125I to specific activities of up to 2 × 108 cpm/mg of protein and with no loss of the colicin biological activity. The labeled colicin bound to colicin E1-sensitive, tolerant, and immune E1-colicinogenic Escherichia coli. An E. coli mutant resistant to colicin E1 exhibited a much lower colicin-binding capacity. The avera
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7. The Tip of the Hydrophobic Hairpin of Colicin U Is Dispensable for Colicin U Activity but Is Important for Interaction with the Immunity Protein
The hydrophobic C terminus of pore-forming colicins associates with and inserts into the cytoplasmic membrane and is the target of the respective immunity protein. The hydrophobic region of colicin U of Shigella boydii was mutated to identify determinants responsible for recognition of colicin U by the colicin U immunity protein. Deletion of the tip of the h
American Society for Microbiology.
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8. Sensitization of Colicin K-Treated Bacteria By Sodium Dodecyl Sulfate: Presence of Free Colicin in Colicin K-Treated Cultures of Escherichia coli
A method of assaying colicin K is described. It makes use of two properties of sodium dodecyl sulfate to protect bacteria against colicin action and to dissolve those bacteria on which colicin K had started its action. By this method, the kinetics of bacterial killing by colicin K have been measured directly in the treated culture without intervening dilutio
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9. Colicin U, a novel colicin produced by Shigella boydii.
A novel colicin, designated colicin U, was found in two Shigella boydii strains of serovars 1 and 8. Colicin U was active against bacterial strains of the genera Escherichia and Shigella. Plasmid pColU (7.3 kb) of the colicinogenic strain S. boydii M592 (serovar 8) was sequenced, and three colicin genes were identified. The colicin U activity gene, cua, enco
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10. Isolation and Characterization of a Mutant Colicin E2
Escherichia coli K-12 colicinogenic for Col E2 yielded a mutant, SK95, that carries a nonsense mutation in the colicin structural gene. A derivative of SK95 that carries an as yet unidentified suppressor mutation produces a colicin E2 that is temperature sensitive (TS). This mutant colicin kills sensitive cells at low temperature but not at high temperature;
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11. Cleavage of colicin Ia by the Escherichia coli K-12 outer membrane is not mediated by the colicin Ia receptor.
Colicin Ia can be cleaved by isolated outer membranes prepared from sensitive and resistant (lacking the colicin Ia receptor) strains of Escherichia coli. Both active and heat-denatured colicin Ia are extensively fragmented. Such proteolysis does not occur when colicin Ia is added to whole sensitive or resistant cells. These results demonstrate that cleavage
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12. Gene expression in vitro of colicin El plasmid.
Among eighteen polypeptides synthesized in vitro from colicin El plasmid, one of the major products with a molecular weight of 59,000 was identified as colicin El by its immunological property, molecular size, and biological activity. In addition to this polypeptide, seven other polypeptides reacted with colicin El antiserum. Using EcoRI-cleaved colicin El D