Complex Mcm2 7
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Análise molecular e citoquímica de genes e proteínas relacionados a osteodiferenciação em células tronco derivadas do tecido adiposo
A descoberta das células tronco adultas tornou possível a sua aplicação clínica em protocolos de medicina regenerativa e de engenharia de tecidos para a reparação de órgãos danificados ou pouco funcionais. Neste sentido, são conhecidas muitas fontes diferentes de células tronco adultas, entre elas o tecido epitelial, o tecido muscular e a medula �
Publicado em: 2009
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2. Mcm10 and the MCM2–7 complex interact to initiate DNA synthesis and to release replication factors from origins
MCM2–7, a complex of six subunits, is an essential component of the prereplication chromatin that is assembled at Saccharomyces cerevisiae replication origins during G1 phase. It is also believed to be the processive helicase at growing forks. To elucidate the action of MCM2–7 during the transition from initiation to elongation replication, we have focus
Cold Spring Harbor Laboratory Press.
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3. Involvement of Human MCM8 in Prereplication Complex Assembly by Recruiting hcdc6 to Chromatin
The MCM2-MCM7 complex is an essential component of the prereplication complex (pre-RC), which is recruited by the cdc6 and cdt1 proteins to origins of DNA replication during G1 phase. Here, we report that the accumulation on chromatin of another member of the MCM protein family, human MCM8 (hMCM8), occurs during early G1 phase, before the hMCM2-hMCM7 complex
American Society for Microbiology.
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4. Biochemical Analysis of the Intrinsic Mcm4-Mcm6-Mcm7 DNA Helicase Activity
Mcm proteins play an essential role in eukaryotic DNA replication, but their biochemical functions are poorly understood. Recently, we reported that a DNA helicase activity is associated with an Mcm4-Mcm6-Mcm7 (Mcm4,6,7) complex, suggesting that this complex is involved in the initiation of DNA replication as a DNA-unwinding enzyme. In this study, we have ex
American Society for Microbiology.
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5. The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase
Previous studies in Saccharomyces cerevisiae have defined an essential role for the Dbf4-Cdc7 kinase complex in the initiation of DNA replication presumably by phosphorylation of target proteins, such as the minichromosome maintenance (Mcm) complex. We have examined the phosphorylation of the Mcm complex by the Dfp1-Hsk1 kinase, the Schizosaccharomyces pombe
The National Academy of Sciences.
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6. Nuclear Localization of Schizosaccharomyces pombe Mcm2/Cdc19p Requires MCM Complex Assembly
The minichromosome maintenance (MCM) proteins MCM2–MCM7 are conserved eukaryotic replication factors that assemble in a heterohexameric complex. In fission yeast, these proteins are nuclear throughout the cell cycle. In studying the mechanism that regulates assembly of the MCM complex, we analyzed the cis and trans elements required for nuclear localizatio
The American Society for Cell Biology.
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7. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases
The minichromosome maintenance (MCM) 2-7 helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability. We describe four lines of evidence that ATM/ATR-dependent (ataxia-telangiectasia-mutated/ATM- and Rad3-related) checkpoint pathways are directly linked
National Academy of Sciences.
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8. Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures
The minichromosome maintenance (Mcm) proteins 2–7 are required for both the initiation and elongation steps of chromosomal DNA replication. Previous studies have shown that the Mcm complex consisting of the Mcm 4, 6, and 7 proteins contains 3′ to 5′ DNA helicase activity with limited processivity (displacing duplex DNA regions up to 30 nt). In thi
The National Academy of Sciences.
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9. Distinct Phosphoisoforms of the Xenopus Mcm4 Protein Regulate the Function of the Mcm Complex
Initiation of DNA replication in eukaryotes requires the assembly of prereplication complexes (pre-Rcs) at the origins of replication. The assembly and function of the pre-Rcs appear to be controlled by phosphorylation events. In this study we report the detailed characterization of the cell cycle phosphorylation of one component of the Xenopus pre-Rcs, the
American Society for Microbiology.
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10. MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication
Mutations in RECQ4, a member of the RecQ family of DNA helicases, have been linked to the progeroid disease Rothmund–Thomson Syndrome. Attempts to understand the complex phenotypes observed in recq4-deficient cells suggest a potential involvement in DNA repair and replication, yet the molecular basis of the function of RECQ4 in these processes remains unkn
Nature Publishing Group.
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11. XMCM7, a novel member of the Xenopus MCM family, interacts with XMCM3 and colocalizes with it throughout replication.
A minichromosome maintenance (MCM) protein complex has been implicated in restricting DNA replication to once per cell cycle in Xenopus egg extracts, based on the behavior of a single protein, XMCM3. Using a two-hybrid screen with XMCM3, we have identified a novel member of the MCM family in Xenopus that is essential for DNA replication. The protein shows st
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12. The Minichromosome Maintenance Proteins 2-7 (MCM2-7) Are Necessary for RNA Polymerase II (Pol II)-mediated Transcription*
The MCM2-7 (minichromosome maintenance) proteins are a family of evolutionarily highly conserved proteins. They are essential for DNA replication in yeast and are considered to function as DNA helicases. However, it has long been shown that there is an overabundance of the MCM2-7 proteins when compared with the number of DNA replication origins in chroma
American Society for Biochemistry and Molecular Biology.