Cryoprotectant
Mostrando 1-12 de 57 artigos, teses e dissertações.
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1. Avaliação morfológica do concentrado autólogo de plaquetas em cães sob refrigeração e criopreservação em DMSO 6%
ABSTRACT The morphological characteristics of the autologous platelet concentrate (APC) of 31 dogs were evaluated after cooling and freezing in 6% DMSO. Blood from the jugular vein of each patient was collected and centrifuged at 191g for six minutes to obtain APC. In the fresh sample, the platelet count, MPV, PDW and cell morphology were evaluated. Four sam
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2020-09
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2. Vitrified embryo transfer in Merino sheep under extensive conditions
Abstract The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were
Anim. Reprod.. Publicado em: 25/11/2019
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3. Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C
Abstract Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl-2-Pyrrolidone (NMP)
Anim. Reprod.. Publicado em: 14/11/2019
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4. Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines
Abstract The present study evaluated the effect of cryoprotectants, semen diluents and chicken lines during pellet method of semen cryopreservation. Three different experiments were conducted; Experiment 1 - semen was cryopreserved using dimethylformamide (DMF) at 6% and 9% concentrations in two semen diluents (Lake and Ravie diluent and TES/NaCl diluent), E
Braz. arch. biol. technol.. Publicado em: 01/08/2019
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5. Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled e
R. Bras. Zootec.. Publicado em: 22/07/2019
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6. Long-term cryopreservation of basidiomycetes
Abstract Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation te
Braz. J. Microbiol.. Publicado em: 2018-06
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7. Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose
Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and t
Braz. J. Microbiol.. Publicado em: 2018-06
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8. Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant
resumo Nanoparticulas lipídicas sólidas (NLSs) são sistemas coloidais de liberação interessantes, uma vez que reúnem todas as vantagens de nanopartículas lipídicas e poliméricas. A liofilização é um processo amplamente utilizado para melhorar a estabilidade das NLSs e os crioprotetores têm sido usados para diminuir a agregação destas durante e
Braz. J. Pharm. Sci.. Publicado em: 2015-12
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9. Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants s
Braz. arch. biol. technol.. Publicado em: 18/03/2014
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10. Medium-term cryopreservation of rabies virus samples
Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of r
Rev. Soc. Bras. Med. Trop.. Publicado em: 2013-12
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11. An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts
PURPOSE: To assess the viability of cultured epithelium and preserved by freezing for periods varying from one month to one year. METHODS: Samples of cultured epithelium were incubated in cryoprotectant medium (Group A), packed in aluminum envelopes and packed in polystyrene boxes. The boxes were subjected to a temperature of-70ºC. After freezing for a peri
Acta Cir. Bras.. Publicado em: 2013-12
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12. Comportamento sexual, parâmetros seminais e diluição pós-congelamento de sêmen de jumentos (Equus asinus) da raça Pêga / Sexual behavior, seminal parameters and dilution after freezing Pêga breed donkeys (Equus asinus) semen
The aim of this study was to elucidate aspects of asinine behavior from the description of various collections using an artificial vagina and relate this behavior with the seminal parameters. We evaluated also the availability of the formulation of the diluent T2-94 plus dimethylformamide in the protection of sperm cells during cryopreservation of semen from
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/03/2012