Cryoprotectants
Mostrando 1-12 de 53 artigos, teses e dissertações.
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1. Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines
Abstract The present study evaluated the effect of cryoprotectants, semen diluents and chicken lines during pellet method of semen cryopreservation. Three different experiments were conducted; Experiment 1 - semen was cryopreserved using dimethylformamide (DMF) at 6% and 9% concentrations in two semen diluents (Lake and Ravie diluent and TES/NaCl diluent), E
Braz. arch. biol. technol.. Publicado em: 01/08/2019
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2. Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled e
R. Bras. Zootec.. Publicado em: 22/07/2019
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3. Cryopreservation of banana’s cv Grand Naine in vitro rhizomes
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryo
An. Acad. Bras. Ciênc.. Publicado em: 23/10/2017
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4. Effects of cryoprotectants and low temperatures on hatching and abnormal embryo development of Prochilodus lineatus (Characiformes: Prochilodontidae)
RESUMO Este estudo avaliou os efeitos dos crioprotetores e das baixas temperaturas no desenvolvimento embrionário de Prochilodus lineatus, descrevendo suas principais alterações morfológicas. Nos testes de sensibilidade ao frio, as taxas de sobrevivência, no estágio de vinte somitos (20S), foram de 53,6% a 0ºC e 100% a 5ºC. Nos testes de toxicidade,
Neotrop. ichthyol.. Publicado em: 17/08/2017
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5. Washings and cryoprotectants for the production of Tilapia Surimi
Abstract This research aimed at studying the effects of different washing conditions (distilled water x distilled water + NaHCO3 + NaCl) and cryoprotectants (NaCl + saccharose x sorbitol + sodium tripolyphosphate) for the production of tilapia surimi. The experiment used a factorial design 2 x 2 and the variables analyzed were proximate composition, non-nitr
Food Sci. Technol. Publicado em: 25/05/2017
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6. Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator (Characiformes: Prochilodontidae)
Este trabalho avaliou o efeito de substâncias crioprotetoras sobre embriões de Prochilodus lineatus em uma incubadora experimental. O estudo aplicou combinações de álcool polivinílico, hidroxietilcelulose, gelatina e soro fetal bovino com dimetilosulfóxido e etilenoglicol em uma nova incubadora experimental. Foram avaliadas a morfologia dos embriões,
Neotrop. ichthyol.. Publicado em: 2014-12
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7. Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants s
Braz. arch. biol. technol.. Publicado em: 18/03/2014
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8. Medium-term cryopreservation of rabies virus samples
Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of r
Rev. Soc. Bras. Med. Trop.. Publicado em: 2013-12
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9. REFRIAMENTO DO SÊMEN DE Colossoma macropomum EM ÁGUA DE COCO EM PÓ (ACP-104) ASSOCIADA À CRIOPROTETORES - ESTUDO DE TOXICIDADE / REFRIAMENTO OF SEMEN Colossoma macropomum WATER COCONUT POWDER (ACP-104) ASSOCIATED cryoprotectants - TOXICITY STUDY
Os peixes da espécie Colossoma macropomum (tambaqui) tem um alto valor econômico e ecológico. Uma tecnologia que vêm se destacando nos últimos anos é a conservação de sêmen realizada por resfriamento. Dessa forma, o estudo tem como objetivo avaliar a qualidade do sêmen de tambaqui em solução de água de coco em pó específico para peixes (ACP-10
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/07/2012
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10. Characterization of lyophilized liposomes produced with non-purified soy lecithin: a case study of casein hydrolysate microencapsulation
A commercial casein hydrolysate was microencapsulated in liposomes produced with non-purified soy lecithin, cryoprotected with two different disaccharides and lyophilized. The encapsulation efficiency of casein hydrolysate ranged from 30 to 40%. The powders were analyzed by differential scanning calorimetry (DSC), scanning electron micrography (SEM), infrare
Brazilian Journal of Chemical Engineering. Publicado em: 2012-06
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11. Efeito crioprotetor de lactose e glicose em cÃlulas fÃngicas imobilizadas em alginato de sÃdio como mÃtodo de preservaÃÃo de culturas. / Cryoprotectant effect of lactose and glucose yeast cells immobilized in calcium alginate as a method for preservation of cultures
Cryopreservation is the method of choice in many banks of microorganisms because it leads to a situation of metabolic dormancy and consequently cultures are stable for long periods. By reducing the temperature may however be formed of ice crystals which tend to cause cell damage to cell damage in turn can be avoided by vitrification process which takes place
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/08/2011
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12. CRIOPRESERVAÇÃO DE TECIDO OVARIANO CAPRINO: EFICIÊNCIA DE DIFERENTES AGENTES CRIOPROTETORES / OVARIAN TISSUE CRYOPRESERVATION GOAT: EFFICIENCY OF DIFFERENT CRYOPROTECTIVE AGENTS
The cryopreservation of ovarian tissue has been the target of intense study with the purpose of assisting in the technology of assisted reproduction in animals and humans. For the success of cryopreservation, the composition of the freezing solution, especially the intracellular cryoprotectant agent used is of fundamental importance. Thus, this study aimed t
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/07/2011