Cultured Cells And Culture Means
Mostrando 1-12 de 39 artigos, teses e dissertações.
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1. Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
RESUMO O objetivo desse estudo foi avaliar a suplementação de meio de cultura de embriões com antioxidante obtido do extrato oleoso da Lippia origanoides no desenvolvimento e na qualidade de blastocistos produzidos in vitro. Oócitos coletados de ovários de matadouros foram maturados e fertilizados in vitro segundo procedimento laboratorial padrão. Zigo
Arq. Bras. Med. Vet. Zootec.. Publicado em: 14/06/2019
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2. Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells
Abstract The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20), in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analysis. Thirty-two round-shaped samples were fabricated with th
Braz. oral res.. Publicado em: 08/03/2016
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3. Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus.Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells.
Mem. Inst. Oswaldo Cruz. Publicado em: 10/10/2013
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4. Estudo comparativo da citotoxicidade de cimentos obturadores sobre culturas de células endoteliais da linhagem ECV 304 / Comparative study of cytotoxicity of sealers on cultured endothelial cells line ECV 304
Este estudo teve como objetivo avaliar, in vitro, a citotoxicidade dos cimentos endodônticos Densell Endo, Pulp-Fill, Endofill, Sealer 26, Pulp Canal Sealer e GuttaFlow após 12, 24 e 72 horas de tempo de contato, utilizando-se uma linhagem de células endoteliais ECV-304. Para a avaliação da viabilidade celular, utilizou-se o teste de citotoxicidade MTT.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/02/2011
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5. Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA.
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2010
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6. Efeito da vitamina D no perfil transcricional de cultura organotípica de câncer de mama / Vitamin D effect in the transcriptional profile of breast cancer organotypic culture
High 1,25(OH)2D3 (VD) concentrations (10-100nM) exerts antiproliferative effects and modifies gene expression profile in breast cancer (BC) cell lines. However, studies conducted in cell lines disconsider stromal-epithelium interactions, which are known to regulate breast cancer development. Besides, high VD concentrations may cause hypercalcemia in vivo. Ou
Publicado em: 2010
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7. Trypanosoma cruzi infection induces up-regulation of cardiac muscarinic acetylcholine receptors in vivo and in vitro
The pathogenesis of chagasic cardiomyopathy is not completely understood, but it has been correlated with parasympathetic denervation (neurogenic theory) and inflammatory activity (immunogenic theory) that could affect heart muscarinic acetylcholine receptor (mAChR) expression. In order to further understand whether neurogenic and/or immunogenic alterations
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-09
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8. A general native-state method for determination of proliferation capacity of human normal and tumor tissues in vitro.
An important need in cancer research and treatment is a physiological means in vitro by which to assess the proliferation capacity of human tumors and corresponding normal tissue for comparison. We have recently developed a native-state, three-dimensional, gel-supported primary culture system that allows every type of human cancer to grow in vitro at more th
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9. Ontogeny of electrically excitable cells in cultured olfactory epithelium.
A primary system has been developed in which it is possible to study the production of electrically excitable neuron-like cells from a precursor population of olfactory epithelial cells. Rat nasal epithelium was dissociated and placed in culture. The initial surviving cells are flat and ciliated and contain glial fibrillary acidic protein (GFAP). After 3-5 d
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10. Electrically conducting polymers can noninvasively control the shape and growth of mammalian cells.
Electrically conducting polymers are novel in that their surface properties, including charge density and wettability, can be reversibly changed with an applied electrical potential. Such properties might render conducting polymers unique for biological applications. However, the majority of research on conducting polymers has been carried out under nonbiolo
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11. Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation.
The expression of Ia-like antigens by cultured human endothelial cells has been investigated by means of monoclonal antibody binding to intact cells and by immunoprecipitation of radioiodinated membrane proteins. Primary growing and confluent cultures of human umbilical vein endothelium express little, if any, detectable Ia-like antigens under standard cultu
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12. Interaction between Borrelia burgdorferi and endothelium in vitro.
During the pathogenesis of Lyme disease, Borrelia burgdorferi spreads hematogenously from the site of a tick bite to several tissues throughout the body. The specific mechanism of spirochete emigration is presently unknown. Using cultured human umbilical vein endothelial cells, we found that Borrelia burgdorferi bound to the endothelial cells and to the sube