Cysteine Endopeptidases
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase
The tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite’s physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzy
Publicado em: 2011
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2. Degradação de vitelina e hemoglobina no carrapato bovino Rhipicephalus (Boophilus) microplus
O presente trabalho caracterizou alguns aspectos enzimáticos da digestão de vitelina e de hemoglobina no carrapato bovino Rhipicephalus (Boophilus) microplus. Um novo protocolo foi desenvolvido para a purificação de uma cisteíno endopeptidase de larvas do carrapato (R. microplus Larval Cysteine Endopeptidase; RmLCE). A seqüência parcial de aminoácido
Publicado em: 2010
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3. Cisteína-proteinases em promastigotas de Leishmania (Viannia) braziliensis. / Cysteine-proteinases in promastigotes of Leishmania (Viannia) braziliensis.
No presente trabalho foram detectadas cisteína-proteinases (CPs) em promastigotas infectivas de Leishmania (Viannia) braziliensis. A estratégia de purificação consistiu na associação do método de extração por Triton X-114 com cromatografia em coluna de Concanavalina A-Sepharose, seguida por outra de DEAE-Sephacel. No ensaio das cromatografias, obser
Publicado em: 2008
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4. A reprodução em carrapatos e a avaliação de uma enzima de destaque neste processo como antígeno vacinal contra Rhipicephalus (Boophilus) microplus
Os carrapatos estão distribuídos por todo mundo e impactam tanto a saúde animal quanto humana. A espécie Rhipicephalus (Boophilus) microplus é o principal parasita que afeta a bovinocultura nas regiões tropicais e subtropicais do globo, causando grandes prejuísos econômicos. Os métodos de controle estão baseados no uso de acaricidas químicos, o qu
Publicado em: 2008
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5. A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein.
A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequent
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6. Identification of active-site residues of the adenovirus endopeptidase.
Multiple sequence alignment of the 12 adenovirus endopeptidases known to date identified a number of conserved residues which might be important for enzyme activity. Eleven mutants were created in the cloned gene by site-directed mutagenesis to identify the active site of this thiol endopeptidase. Analysis of the proteolytic activity in a crude system using
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7. Multiple Forms of Acidic Endopeptidase from Germinated Barley 1
An endopeptidase preparation from germinated barley Hordeum vulgare L., cv. Trophy, purified by affinity chromatography and density-gradient electrofocusing, consisted of three or four components. The preparation was only partly resolved by electrofocusing, with evidence of three possible components (pI 4.15, 4.28, and 4.37). Gel filtration on Sephadex G-75
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8. Exploiting Secondary Growth in Arabidopsis. Construction of Xylem and Bark cDNA Libraries and Cloning of Three Xylem Endopeptidases1
The root-hypocotyl of Arabidopsis produces a relatively large amount of secondary vascular tissue when senescence is delayed by the removal of inflorescences, and plants are grown at low population density. Peptidase zymograms prepared from isolated xylem and phloem revealed the existence of distinct proteolytic enzyme profiles within these tissues. cDNA lib
American Society of Plant Physiologists.
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9. Molecular and Biotechnological Aspects of Microbial Proteases†
Proteases represent the class of enzymes which occupy a pivotal position with respect to their physiological roles as well as their commercial applications. They perform both degradative and synthetic functions. Since they are physiologically necessary for living organisms, proteases occur ubiquitously in a wide diversity of sources such as plants, animals,
American Society for Microbiology.