Cytochemistry Activity
Mostrando 1-12 de 29 artigos, teses e dissertações.
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1. In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering
The objective of this study was to evaluate Vero cells in in vitro biocompatibility tests with pure PHBV and PCL and 75/25 and 50/50 blends developed for bone tissue bioengineering in dense or porous forms. The biomaterials were characterized morphologically by scanning electron microscopy, (SEM) stereoscopic imaging (SI), and micrometer measurement. Cellula
Mat. Res.. Publicado em: 25/11/2019
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2. Avaliação e demonstração da atividade citocromo C oxidase em espermatozóides bovinos.
1997
ARS VETERINÁRIA v.13. Publicado em: 2011
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3. Regulação hormonal da prostata de femeas do gerbilo : avaliação estrutural, citoquimica e imunocitoquimica / Hormonal regulation of the gerbil female propstate: morphology, cytochemistry and immunocytochemistry
The female prostate is a functionally active gland found in several species of mammals, including humans and rodents. In adult female gerbils, the prostate presents a paraurethral location, showing close contact with the wall of urethra in its median and distal portions. This gland is homologue to the ventral prostate of male rodents and it is formed by a cl
Publicado em: 2006
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4. Dehydrogenase enzyme cytochemistry of unfixed leucocytes
An inert, synthetic, sucrose polymer (Ficoll) has been found to have a protective effect on leucocytes during incubation for cytochemical reactions at 37°C. This permits enzyme cytochemistry to be performed on unfixed bone marrow and peripheral blood smears, and on leucocytes cultured in vitro, thus avoiding enzyme inhibition by the use of a fixative. The t
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5. Quantitative enzyme cytochemistry of leukaemic cells
A new technique for the quantitative estimation of glycolytic and respiratory enzyme activity in intact and unfixed bone marrow and peripheral blood cells is described. The method gives a two- to three-fold increase in demonstrable enzyme activity per cell compared with existing techniques using fixed marrow film preparations, and is particularly applicable
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6. Cytochemistry of blood cells in two West African amphibians.
Lipid, glycogen, peroxidase, acid and alkaline phosphatases were studied in the cells of blood smears in the toad and frog. While lipid and glycogen were largely confined to granulocytes and monocytes, peroxidase activity was intense in all cell types except lymphocytes in both species.
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7. Adenylate Cyclase Activity Not Found in Soybean Hypocotyl and Onion Meristem 1
Tissue, homogenates, and purified cell fractions prepared from hypocotyls of a dicot, soybean (Glycine max), and meristematic tissue of a monocot, onion (Allium cepa), were examined critically for evidence of adenylate cyclase activity. Three assay methods were used: chemical analysis, isotope dilution analysis, and enzyme cytochemistry. In both crude extrac
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8. Metabolic alterations in human synovial lining cells in pigmented villonodular synovitis.
Synovial tissue from 3 patients with villonodular synovitis has been examined by quantitative cytochemistry. Considerable changes in the metabolism of the synoviocytes have been observed. These included a massive rise in the activity of glucose 6-phosphate dehydrogenase to levels even greater than those found in rheumatoid arthritis, and a significant rise i
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9. Mitochondrial oxidative activity in human rheumatoid synovial lining cells.
The activities of two mitochondrial enzymes, succinate dehydrogenase and cytochrome oxidase, have been measured by quantitative cytochemistry and microdensitometry in the synoviocytes of rheumatoid and non-rheumatoid synovial lining cells. Although both tended to be higher in the former, there was no statistically significant difference in the activities of
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10. Relation of neutrophil alkaline phosphatase activity to Fc IgG receptor development in human blood and bone marrow.
A possible correlation between Fc-IgG receptor expression and neutrophil alkaline phosphatase (NAP) activity was investigated in relation to maturation of granulocytes in human peripheral blood and bone marrow. NAP activity was studied in bone marrow from patients with normal peripheral blood NAP scores (20-100) and compared with those with high NAP scores.
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11. Cytochemistry of cytochrome oxidase in the cytoplasmic and intracytoplasmic membranes of Azotobacter vinelandii.
Vegetative cells of Azotobacter vinelandii contain a system of intracytoplasmic membranes in the form of numerous internal vesicles. The three-dimensional morphology of these internal vesicles was established by an examination of stereopair electron micrographs of negatively stained cells. The vesicles assumed a variety of forms ranging from nearly spherical
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12. Cytochemical techniques and energy-filtering transmission electron microscopy applied to the study of parasitic protozoa
The study of parasitic protozoa plays a major role in cell biology, biochemistry and molecular biology. Numerous cytochemical techniques have been developed in order to unequivocally identify the nature of subcellular compartments. Enzyme and immuno-cytochemistry allow the detection of, respectively, enzymatic activity products and antigens in particular sit
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