Dextranase
Mostrando 1-12 de 54 artigos, teses e dissertações.
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1. Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice
ABSTRACT The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.
An. Acad. Bras. Ciênc.. Publicado em: 2018-03
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2. Produção, purificação e caracterização bioquímica de dextranase de Paecilomyces marquandii / Production, purification and biochemical characterization of dextranase from Paecilomyces marquandii
Dextranases (EC 3.2.1.-) catalisam a hidrólise de ligações glicosídicas α(1-6) em polissacarídios de dextrana e são produzidas por fungos, bactérias e algumas leveduras. São utilizadas na eliminação de dextranas produzidas por bactérias que podem ocasionar sérios problemas ao longo de todo o processamento de açúcar, na prevenção e tratam
Publicado em: 2009
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3. Produção, purificação, caracterização e viabilidade de aplicação da dextranase de penicillium SP
Oitocentas e sessenta e três linhagens de fungos foram isoladas do solo de plantações de cana de açúcar, assim como de varas de cana deterioradas, e testadas quanto ã atividade dedextranase. Nesta seleção, foi encontrada uma linhagem de Penicillium sp que produz alta atividade de dextranase quando cultivada em meio líquido contendo dextrana como ún
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/01/1987
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4. More sensitive test agar for detection of dextranase-producing oral streptococci and identification of two glucan synthesis-defective dextranase mutants of Streptococcus mutans 6715.
A more sensitive test agar was developed to detect oral microbes with relatively low dextranase activity and to identify dextranase-negative mutants. Several oral streptococci that had previously been scored as dextranase negative readily decolorized the new, blue dextran-containing medium. To assess whether dextranase plays a role in glucan synthesis by ora
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5. Characterization of an Extracellular Dextranase from Fusarium moniliforme
An extracellular dextranase (EC 3.2.1.11) was purified approximately 75-fold from cell-free culture filtrates of Fusarium moniliforme. The purified dextranase was of the endo type, and isomaltose was identified as the primary end product of dextran hydrolysis. The molecular weight of the dextranase was determined to be 39,000 by gel permeation chromatography
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6. Effects of Dextranases on Attachment of Streptococcus mutans to Hydroxyapatite
A Fusarium dextranase and a Penicillium dextranase were compared for their relative ability to quantitatively reduce the adsorption of 3H-labeled Steptococcus mutans cells onto hydroxyapatite. Fusarium dextranase-treated hydroxyapatite disks caused a statistically significant decrease in the hydroxyapatite adsorption of both the OMZ 176 and NCTC 10449 strain
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7. Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sobrinus and cloning of the dextranase gene.
The presence of proteases in culture supernatant fluids and on the cell surface of Streptococcus sobrinus and the aggregation of multicomponent enzyme complexes make the isolation and characterization of cell surface proteins difficult. We report a simple purification procedure for dextranase and the cloning of the dextranase structural gene. S. sobrinus cul
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8. Characterization of a dextranase produced by an oral strain of Actinomyces israelii.
A dextranase-producing, gram-positive, anaerobic, rod-shaped bacterium isolated from human dental plaque was identified as Actinomyces israeli. Although the extracellular dextranase (EC 3.2.1.11) formed by this microbe appeared to be constitutively produced, the bacterium did not utilize the reaction products as a carbon source during growth. A striking feat
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9. Dextranase enhances antibiotic efficacy in experimental viridans streptococcal endocarditis.
In endocarditis, exopolysaccharide production by viridans streptococci has been associated with delayed antimicrobial efficacy in cardiac vegetations. We compared the efficacies of temafloxacin alone and in combination with dextranase, an enzyme capable of hydrolyzing 20 to 90% of the bacterial glycocalyx, in a rabbit model of endocarditis. In in vivo experi
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10. Overproduction of a dextranase inhibitor by Streptococcus sobrinus mutants.
An inhibitor of Streptococcus sobrinus endodextranase was detected in the extracellular fractions of UAB66 mutants identified following ethyl methanesulfonate mutagenesis as either devoid of dextranase activity (Dex-) or overproducing water-soluble glucan. The two groups of mutants had the same phenotype and displayed no dextranase activity in assays of extr
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11. Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii.
We have constructed a plasmid to express and secrete dextranase in the oral bacterium Streptococcus gordonii. The dextranase gene from Arthrobacter sp. strain CB-8 was linked to a promoter and a DNA sequence encoding the signal peptide of Streptococcus downei glucosyltransferase I (gtfI) followed by the Escherichia coli rrnBt1t2 terminator and inserted in th
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12. Molecular cloning of the extracellular endodextranase of Streptococcus salivarius.
We report the cloning in Escherichia coli of the gene encoding an extracellular endodextranase (alpha-1,6-glucanhydrolase, EC 3.2.1.11) from Streptococcus salivarius PC-1. Recombinants from a S. salivarius PC-1-Lambda ZAP II genomic library specifying dextranase activity were identified as plaques surrounded by zones of clearing on blue dextran agar. One suc