Dichelobacter Nodosus
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Evolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina
Neste artigo realizou-se a avaliação de ovinos mestiços Santa Inês com pododermatite infecciosa, verificando as alterações no leucograma e proteínas de fase aguda. No primeiro experimento, 70 fêmeas foram separadas em três grupos de acordo com o seu escore podal: Grupo controle (G1) com escore 0; Dermatite interdigital (G2) com escore 1 ou 2 e Podod
Pesq. Vet. Bras.. Publicado em: 2012-12
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2. Pododermatite infecciosa e outras doenças podais de caprinos e ovinos no semiárido Nordestino
Este trabalho relata a ocorrência e aspectos epidemiológicos de surtos de pododermatite infecciosa e outras afecções podais em caprinos e ovinos no semiárido paraibano. As propriedades foram inspecionadas quanto à presença de problemas podais e aspectos relacionados às condições ambientais, higiene, locais de pastejo e medidas de controle utilizada
Pesquisa Veterinária Brasileira. Publicado em: 2011-10
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3. Controle de Footrot em reanho ovino no estado do Rio Grande do Sul: uso de vacina autógena e resposta sorológica
O objetivo desta dissertação de mestrado foi avaliar a eficácia de uma vacina autógena no controle do footrot (FR) dos ovinos, para tanto foram desenvolvidos dois experimentos em duas propriedades rurais distintas, no Estado do Rio Grande do Sul (RS). O primeiro experimento foi conduzido em uma propriedade rural do município de Santiago, com um extenso
Publicado em: 2010
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4. Shared antigenicity and immunogenicity of type 4 pilins expressed by Pseudomonas aeruginosa, Moraxella bovis, Neisseria gonorrhoaea, Dichelobacter nodosus, and Vibrio cholerae.
Immunoblotting with polyclonal rabbit antibodies raised against pilins expressed by Pseudomonas aeruginosa, Moraxella bovis, Neisseria gonorrhoeae, Dichelobacter nodosus, and Vibrio cholerae was used to demonstrate that these polypeptides display conserved antigenic and, in most cases, immunogenic determinants. These determinants appear to be localized to th
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5. Molecular characterization of a genomic region associated with virulence in Dichelobacter nodosus.
The major pathogen implicated in footrot, a highly contagious disease of sheep, is the strict anaerobe Dichelobacter nodosus (formerly Bacteroides nodosus). Sequence analysis of a 2,262-bp segment of the D. nodosus genome which is more prevalent in virulent isolates than in other isolates showed the presence of four open reading frames which appeared to have
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6. Complete Nucleotide Sequence of the 27-Kilobase Virulence Related Locus (vrl) of Dichelobacter nodosus: Evidence for Extrachromosomal Origin
The vrl locus is preferentially associated with virulent isolates of the ovine footrot pathogen, Dichelobacter nodosus. The complete nucleotide sequence of this 27.1-kb region has now been determined. The data reveal that the locus has a G+C content much higher than the rest of the D. nodosus chromosome and contains 22 open reading frames (ORFs) encoding pro
American Society for Microbiology.
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7. The Type IV Fimbrial Subunit Gene (fimA) of Dichelobacter nodosus Is Essential for Virulence, Protease Secretion, and Natural Competence
Dichelobacter nodosus is the essential causative agent of footrot in sheep. The major D. nodosus-encoded virulence factors that have been implicated in the disease are type IV fimbriae and extracellular proteases. To examine the role of the fimbriae in virulence, allelic exchange was used to insertionally inactivate the fimA gene, which encodes the fimbrial
American Society for Microbiology.
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8. Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets
The expression of iron regulated genes in bacteria is typically controlled by the ferric uptake regulator (Fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, and virulence. We have identified a fur homologue in Dichelobacter nodosus, the causative agent of ovine footrot, and shown that
American Society for Microbiology.
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9. Genetic organization of the duplicated vap region of the Dichelobacter nodosus genome.
The recombinant plasmid pJIR318 contains a fragment of the Dichelobacter nodosus genome which is associated with virulence. Sequence analysis of the pJIR318 insert has shown that it contains four vap (virulence-associated protein) genes which are homologous to open reading frames found on the Escherichia coli F plasmid and the Neisseria gonorrhoeae cryptic p
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10. Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus.
The termination of protein synthesis in bacteria requires two codon-specific release factors, RF-1 and RF-2. A gene for a third factor, RF-3, that stimulates the RF-1 and RF-2 activities has been isolated from the gram-negative bacteria Escherichia coli and Dichelobacter nodosus. In this work, we isolated the RF-3 gene from Salmonella typhimurium and compare
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11. Complementation Analysis of the Dichelobacter nodosus fimN, fimO, and fimP Genes in Pseudomonas aeruginosa and Transcriptional Analysis of the fimNOP Gene Region
The causative agent of ovine footrot, the gram-negative anaerobe Dichelobacter nodosus, produces polar type IV fimbriae, which are the major protective antigens. The D. nodosus genes fimN, fimO, and fimP are homologs of the Pseudomonas aeruginosa fimbrial assembly genes, pilB, pilC, and pilD, respectively. Both the pilD and fimP genes encode prepilin peptida
American Society for Microbiology.
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12. Expression and secretion of heterologous proteases by Corynebacterium glutamicum.
Genes encoding the basic protease of Dichelobacter nodosus (bprV) and the subtilisin of Bacillus subtilis (aprE) were cloned and expressed in Corynebacterium glutamicum. In each case, enzymatically active protein was detected in the supernatants of liquid cultures. While the secretion of subtilisin was directed by its own signal peptide, the natural signal p