Dna Microinjection
Mostrando 1-12 de 260 artigos, teses e dissertações.
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1. Produção de embriões pró-nucleares em fêmeas doadoras das raças Canindé e Saanen durante um programa de transgênese caprina. / Production of embryos pro-nuclear donor females and Saanen breeds Canindé during a program of transgenic goats.
A produção eficiente e a qualidade dos embriões pró-nucleares são fundamentais para o sucesso de um programa de transgênese caprina. Assim, este trabalho teve por objetivo comparar a produção quanti-qualitativa de embriões pró-nucleares em duas raças caprinas para posterior microinjeção de DNA. Para tanto, foram utilizadas fêmeas das raças Saa
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/12/2008
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2. Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy
In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy whe
Genetics and Molecular Biology. Publicado em: 2007
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3. Transferência gênica mediada por espermatozóides para geração de jundiás (Rhamdia quelen) transgênicos / Sperm Mediated Gene Transfer for generation of transgenic jundiás (Rhamdia quelen)
Various methodologies to insert exogenous DNA into eukaryotic cells have been studied in the last two decades. In fish, the technique most studied to the moment is microinjection, which promotes the insertion of fragments of interest genes into zygotes. However, this methodology has some negative aspects: it is time and work consuming, requires qualified lab
Publicado em: 2004
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4. Regulatory mechanism of simian virus 40 gene expression in permissive and in nonpermissive cells.
Primary or continuous lines of mouse cells (3T3) are nonpermissive for simian virus 40 (SV40). Abortively infected cells synthesize tumor antigen (T antigen but not viral DNA and virus capsid protein (V antigen). V antigen, however, was obtained when SV40 DNA was injected into 3T3 cells. This late gene expression also appears to be correlated with the quanti
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5. Hemimethylation of DNA prevents chromatin expression.
The activity of hemimethylated herpes simplex virus thymidine kinase DNA and chromatin was analyzed by microinjection and thymidine incorporation into the DNA of thymidine kinase-negative Rat2 cells. Hemimethylated DNA was obtained by in vitro replication of single-stranded M13 DNA constructs and of chromatin produced by in vitro reconstitution of the DNA wi
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6. Induction of transformation and DNA synthesis after microinjection of raf proteins.
Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3 cells induced DNA synthesis and morphological transformatio
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7. Transient complementation of xeroderma pigmentosum cells by microinjection of poly(A)+ RNA.
An assay has been developed in which excision repair deficiency of xeroderma pigmentosum cells is transiently complemented, as measured by unscheduled DNA synthesis, by microinjection of cytoplasmic poly(A)+ RNA derived from HeLa cells. Four different complementation groups of xeroderma pigmentosum have been assayed. Groups A and G showed complementation, wh
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8. Differences in intracellular DNA ligation after microinjection and transfection.
An uninterrupted avian sarcoma viral genome terminated by viral long terminal repeat sequences was cloned into a pBR322 plasmid. After introduction into a cultured avian cell, transcription of either the circular plasmid molecule or one linearized within the pBR322 sequences could initiate and terminate at long terminal repeat sequences, yielding full-sized
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9. The release of growth arrest by microinjection of adenovirus E1A DNA.
The induction of DNA synthesis in growth-arrested mouse fibroblasts (NIH 3T3) was studied by microinjection of different constructs of adenovirus DNA using SV40 DNA and plasmid DNA as positive and negative controls. The E1A region of adenovirus types 2 and 12 appears to be sufficient to induce cellular DNA synthesis after growth arrest in approximately 30% o
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10. Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA.
Gene expression of Epstein-Barr virus (EBV) was studied after microinjection of viral DNA into different types of cells. Raji TK- cells, known to express viral gene functions after superinfection with the EBV-P3HR-1 virus strain, were attached to plastic dishes by using anti-lymphocyte IgG, phytohemagglutinin, or concanavalin A as a ligand. It was difficult
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11. Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.
We have developed a procedure that gives a very high efficiency of transfection in mammalian cells with low-molecular-weight DNA (approximately 10(4) base pairs). The procedure uses cells in suspension that are shocked with polyethylene glycol 4 h after replating. We compared this transfection technique to the standard technique involving manual microinjecti
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12. Cellular mutation mediates T-antigen-positive revertant cells resistant to simian virus 40 transformation but not to retransformation by polyomavirus and adenovirus type 2.
T-antigen-positive transformation revertant cell lines were isolated from fully simian virus 40 (SV40)-transformed Fisher rat embryo fibroblast cells (REF 52 cells) by methionine starvation. Reversion of the transformed cells (SV-52 cells) was caused by a mutation within the cellular genome. To demonstrate this, we isolated SV40 DNA from the host genome, ins