Embryogenic
Mostrando 1-12 de 112 artigos, teses e dissertações.
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1. Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification
RESUMO: O objetivo desse estudo foi avaliar o efeito das soluções de vitrificação e do tempo de exposição na criopreservação de plúmulas de coqueiro anão verde do Brasil de Jiqui (BGD), pela técnica de vitrificação em gotas. Os explantes foram excisados de frutos maduros oriundos do Banco de Germoplasma Ativo de Embrapa Tabuleiros Costeiros, Se
Cienc. Rural. Publicado em: 13/12/2019
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2. Anatomical and histochemical studies of the somatic embryogenesis of Syagrus oleracea from immature inflorescences
Abstract Anatomical and histochemical studies were carried out during the somatic embryogenesis of Syagrus oleracea (Mart.) Becc. from immature inflorescences. Immature rachillas were inoculated on Murashige and Skoog medium with 4.52 μM 4-amino-3,5,6-trichloropicolinic acid (Picloram) and 2,4-diclorophenoxyacetic acid. Auxin concentrations were gradually r
Crop Breed. Appl. Biotechnol.. Publicado em: 05/12/2019
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3. AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus
RESUMO O objetivo do estudo foi avaliar o efeito de intervalos de pulsos de auxina na indução de embriões somáticos de Eucalyptus grandis x E. urophylla e a descrição do comportamento embriogênico de calos sob efeito do estresse auxínico. Cotilédones foram inoculados em meio de cultura contendo 207,07 µM de picloram, tratamento considerado como pul
Rev. Árvore. Publicado em: 07/10/2019
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4. HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)
RESUMO O dendezeiro é economicamente importante pela alta produção de óleo. A embriogênese somática indireta em dendezeiro demanda longo tempo de indução de calos e formação das plantas, sendo importante o estudo do potencial embriogênico dos calos e os mecanismos da embriogênese somática. O objetivo deste estudo foi verificar o efeito de difere
Rev. Árvore. Publicado em: 23/09/2019
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5. Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants
Abstract: Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vit
An. Acad. Bras. Ciênc.. Publicado em: 19/08/2019
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6. Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms
RESUMO: O presente estudo buscou induzir a formação de embriões somáticos em protocormos do híbrido Phalaenopsis Classic Spotted Pink em dois estádios de maturação fisiológica aos 80 e 120 dias após a inoculação das sementes (DAIS). Os protocormos foram inoculados em meio ½ MS suplementado com 0,1 mg L-1 de ANA e 3 mg L-1 de TDZ. Aos 15 dias de
Cienc. Rural. Publicado em: 04/07/2019
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7. Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis
ABSTRACT The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the gr
Braz. arch. biol. technol.. Publicado em: 14/11/2018
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8. The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos
Abstract This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya ‘Golden THB’. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chloro
An. Acad. Bras. Ciênc.. Publicado em: 25/10/2018
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9. Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
ABSTRACT Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an
An. Acad. Bras. Ciênc.. Publicado em: 2018-04
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10. Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects
ABSTRACT The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturati
An. Acad. Bras. Ciênc.. Publicado em: 01/02/2018
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11. Tissue culture efficiency of wheat species with different genomic formulas
Abstract Ancient wheats are increasingly considered as valuable resources for genes of interest which could be analyzed and introduced into cultivated varieties by genetic engineering technologies. The first stage of biotechnological crop improvement consists of successful in vitro plant regeneration. Twelve wheat germplasms with different genomic formulas (
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-12
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12. Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera
Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of thes
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-09