Engineered Surface
Mostrando 1-12 de 137 artigos, teses e dissertações.
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1. Conformação mecânica: efeito da topografia na tansferência de material e no atrito. / Metal forming: the effect of topography on the metal transfer and friction.
Com a evolução dos processos de fabricação, aumenta o emprego de topografias engenheiradas com o objetivo de aumentar o desempenho dessas superfícies sob vários aspectos. Apesar do bom desempenho comprovado em algumas aplicações, existem outras em que o desempenho é questionável, como, em condições não lubrificadas e com elevadas pressões de co
Publicado em: 2010
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2. Sol-Gel SiO2-CaO-P2O5 biofilm with surface engineered for medical application
Sol-gel film in the SiO2-CaO-P2O5 system was prepared from TEOS, TEP, alcohol and hydrated calcium nitrate in an acidic medium. The coatings were deposited on stainless steel using the dip-coating technique. After deposition, the composite was submitted to heat treatment, at different temperatures and exposure times to investigate the influence of such param
Materials Research. Publicado em: 2007-06
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3. "Propriedades estruturais de agregados anfifílicos catiônicos: interação com material genético" / structural properties of cationic amphiphilic aggregates: interaction with genetic material
Though less efficient than viral vectors, cationic lipids have been used as carriers in gene therapy. They offer several advantages over viral vectors, including the low immunogenic and inflammatory responses, the potential transfer of unlimited-size expression units, and the possibility for engineered cell-specific targeting. However, the interaction betwee
Publicado em: 2006
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4. Generation of Metal-Binding Staphylococci through Surface Display of Combinatorially Engineered Cellulose-Binding Domains
Ni2+-binding staphylococci were generated through surface display of combinatorially engineered variants of a fungal cellulose-binding domain (CBD) from Trichoderma reesei cellulase Cel7A. Novel CBD variants were generated by combinatorial protein engineering through the randomization of 11 amino acid positions, and eight potentially Ni2+-binding CBDs were s
American Society for Microbiology.
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5. Potent antitumor activity of a urokinase-activated engineered anthrax toxin
The acquisition of cell-surface urokinase plasminogen activator activity is a hallmark of malignancy. We generated an engineered anthrax toxin that is activated by cell-surface urokinase in vivo and displays limited toxicity to normal tissue but broad and potent tumoricidal activity. Native anthrax toxin protective antigen, when administered with a chimeric
The National Academy of Sciences.
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6. Expression of functional Porphyromonas gingivalis fimbrillin polypeptide domains on the surface of Streptococcus gordonii.
Genetically engineering bacteria to express surface proteins which can antagonize the colonization of other microorganisms is a promising strategy for altering bacterial environments. The fimbriae of Porphyromonas gingivalis play an important role in the pathogenesis of periodontal diseases. A structural subunit of the P. gingivalis fimbriae, fimbrillin, has
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7. Enhanced Mercury Biosorption by Bacterial Cells with Surface-Displayed MerR
The metalloregulatory protein MerR, which exhibits high affinity and selectivity toward mercury, was exploited for the construction of microbial biosorbents specific for mercury removal. Whole-cell sorbents were constructed with MerR genetically engineered onto the surface of Escherichia coli cells by using an ice nucleation protein anchor. The presence of s
American Society for Microbiology.
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8. Specific Adhesion to Cellulose and Hydrolysis of Organophosphate Nerve Agents by a Genetically Engineered Escherichia coli Strain with a Surface-Expressed Cellulose-Binding Domain and Organophosphorus Hydrolase
A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and a cellulose-binding domain (CBD) on the cell surface was constructed, enabling the simultaneous hydrolysis of organophosphate nerve agents and immobilization via specific adsorption to cellulose. OPH was displayed on the cell surface by use of the truncated ic
American Society for Microbiology.
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9. Surface Modifications Created by Using Engineered Hydrophobins
Hydrophobins are small (ca. 100 amino acids) secreted fungal proteins that are characterized by the presence of eight conserved cysteine residues and by a typical hydropathy pattern. Class I hydrophobins self-assemble at hydrophilic-hydrophobic interfaces into highly insoluble amphipathic membranes, thereby changing the nature of surfaces. Hydrophobic surfac
American Society for Microbiology.
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10. Genetically Controlled Self-Aggregation of Cell-Surface-Engineered Yeast Responding to Glucose Concentration
We constructed an arming (cell-surface-engineered) yeast displaying two types of agglutinin (modified a-agglutinin and α-agglutinin) on the cell surface, with agglutination being independent of both mating type and pheromones. The modified a-agglutinin was artificially prepared by the fusion of the genes encoding Aga1p and Aga2p. The modified a-agglutinin c
American Society for Microbiology.
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11. Migration through the Extracellular Matrix by the Parasitic Protozoan Leishmania Is Enhanced by Surface Metalloprotease gp63
Leishmania species engineered to express high levels of the surface metalloprotease gp63 have enhanced capacity of migration through extracellular matrix in vitro. This correlates with gp63 degradation of extracellular matrix components, such as collagen type IV and fibronectin, and suggests an important role for gp63 in the pathogenesis of leishmaniasis.
American Society for Microbiology.
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12. Metabolic Engineering of an Aerobic Sulfate Reduction Pathway and Its Application to Precipitation of Cadmium on the Cell Surface
The conversion of sulfate to an excess of free sulfide requires stringent reductive conditions. Dissimilatory sulfate reduction is used in nature by sulfate-reducing bacteria for respiration and results in the conversion of sulfate to sulfide. However, this dissimilatory sulfate reduction pathway is inhibited by oxygen and is thus limited to anaerobic enviro
American Society for Microbiology.