Enteric Tropism
Mostrando 1-7 de 7 artigos, teses e dissertações.
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1. Estudo da interação do adenovírus humano, sorotipo 41 (HAdV-41), com células permissivas. / Interaction studies of human adenovirus serotype 41 (HAdV-41) with permissive cells.
Com o objetivo de estudar a interação do HAdV-41 com células permissivas, primeiramente foi observada a cinética de infecção do HAdV-41 em células HEK-293, durante 7 dias. A seguir, as culturas foram analisadas por MCVL e por MET. O HAdV-41 apresentou um ciclo replicativo lento com liberação da progênie viral por mecanismo não lítico. A seguir, c
Publicado em: 2008
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2. Avian coronaviruses: detection, molecular and phylogenetic characterization and experimental inoculation in SPF birds / Coronavirus em aves: detecção, caracterização molecular e filogenética e inoculação experimental em aves SPF
In a broiler breeder farm located at São Paulo State two samples of intestinal contents of 2-week old birds, four fecal samples of the floor from the same flock at 18 weeks of age and one at 30 weeks of age were surveyed to coronavirus by a nested RT-PCR assay targeted to the RNA-dependent RNA-polymerase gene (pol gene). Six out of these samples were positi
Publicado em: 2004
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3. Targeted Recombination Demonstrates that the Spike Gene of Transmissible Gastroenteritis Coronavirus Is a Determinant of Its Enteric Tropism and Virulence
Targeted recombination within the S (spike) gene of transmissible gastroenteritis coronavirus (TGEV) was promoted by passage of helper respiratory virus isolates in cells transfected with a TGEV-derived defective minigenome carrying the S gene from an enteric isolate. The minigenome was efficiently replicated in trans and packaged by the helper virus, leadin
American Society for Microbiology.
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4. Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and Macrophages
Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with e
Public Library of Science.
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5. In Vitro and In Vivo Studies of the Yrp1 Protease from Yersinia ruckeri and Its Role in Protective Immunity against Enteric Red Mouth Disease of Salmonids
Yersinia ruckeri, the etiological agent of the enteric red mouth disease (ERM) of salmonids, produces Yrp1, a serralysin metalloprotease involved in pathogenesis. We describe here the hydrolytic and immunogenic properties of Yrp1. The protease was able to hydrolyze different matrix and muscle proteins as laminin, fibrinogen, gelatine, actin, and myosin but n
American Society for Microbiology.
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6. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome
The construction of cDNA clones encoding large-size RNA molecules of biological interest, like coronavirus genomes, which are among the largest mature RNA molecules known to biology, has been hampered by the instability of those cDNAs in bacteria. Herein, we show that the application of two strategies, cloning of the cDNAs into a bacterial artificial chromos
The National Academy of Sciences.
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7. Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs.
Some characteristics of a newly recognized porcine enteric virus are described. Tentatively, the virus was referred to as porcine pararotavirus (PaRV) because it resembled rotaviruses in respect to size, morphology, and tropism for villous enterocytes of the small intestine. However, it was antigenically distinct from porcine, human, and bovine rotaviruses a