Entire Genome Sequencing
Mostrando 1-12 de 80 artigos, teses e dissertações.
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1. Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain
BACKGROUND Leptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates. OBJECTIVES This study aimed to obtain the complete
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-02
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2. Approaches for in silico finishing of microbial genome sequences
Abstract The introduction of next-generation sequencing (NGS) had a significant effect on the availability of genomic information, leading to an increase in the number of sequenced genomes from a large spectrum of organisms. Unfortunately, due to the limitations implied by the short-read sequencing platforms, most of these newly sequenced genomes remained as
Genet. Mol. Biol.. Publicado em: 2017-09
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3. Impact of NGS in the medical sciences: genetic syndromes with an increased risk of developing cancer as an example of the use of new technologies
The increased speed and decreasing cost of sequencing, along with an understanding of the clinical relevance of emerging information for patient management, has led to an explosion of potential applications in healthcare. Currently, SNP arrays and Next-Generation Sequencing (NGS) technologies are relatively new techniques used to scan genomes for gains and l
Genet. Mol. Biol.. Publicado em: 2014
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4. Full-length genomic sequence of hepatitis B virus genotype C2 isolated from a native Brazilian patient
The hepatitis B virus (HBV) is among the leading causes of chronic hepatitis, cirrhosis and hepatocellular carcinoma. In Brazil, genotype A is the most frequent, followed by genotypes D and F. Genotypes B and C are found in Brazil exclusively among Asian patients and their descendants. The aim of this study was to sequence the entire HBV genome of a Caucasia
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-06
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5. Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja / Identification and validation of indel and SNP molecular markers for soybean seed lipoxygenases
The oxidative degradation of fatty acids from soybean oil fraction, that occurs during the grain processing, develop a flavor of unripe been that modify the palatability and therefore the acceptability of soybean products, resulting in problems for the food industry. The enzyme lipoxygenases (linoleate: O2 oxidoreductase, EC 1.13.11.12), that catalyses this
Publicado em: 2009
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6. Respiratory infections by human bocavirus: molecular and clinical features. / Infecções respiratórias por bocavirus humano: aspectos clínicos e moleculares
O bocavirus humano (HBoV) é um parvovirus recentemente identificado em associação com a presença de sintomas de infecção do trato respiratório. Esse vírus possui um genoma de aproximadamente 5217 nucleotídeos que contém 3 open reading frames que codificam 4 proteínas (NS1, NP-1, VP-1 e VP-2). HBoV tem sido detectado em amostras respiratórias de d
Publicado em: 2009
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7. Sequencing and genome annotation of the party of Xylella fastidiosa / Seqüenciamento e anotações de parte do genoma de Xylella fastidiosa
The São Paulos citriculture can be considered one of the most competitive and important agroindustrial activity from Brazil. It provides aproximately 400,000 jobs and adds US$ 1,4000,000,000 for the countrys economy. This activity, however, still shows less productivity than the one from Florida, mainly due to nutritional and hydric deficiencies and plagues
Publicado em: 2001
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8. Molecular and biological characterization of a begomovirus isolated from tomato, Lycopersicon esculentum Mill. in the state of Goiás and its interaction with the vector Bemisia argentifolii Bellows &Perring / Caracterização molecular e biológica de um begomovírus isolado de tomateiro, Lycopersicon esculentum Mill., no estado de Goiás e sua interação com o vetor Bemisia argentifolii Bellows &Perring
The whitefly-transmitted viruses from the family Geminiviridae, genus Begomovirus, have been reported as an economically important pathogen group that affect important crops in tropical and subtropical countries. Since the beginning of the 1980 decade, the occurrence of the whitefly associated to Begomovirus infection has drastically increased worldwide. In
Publicado em: 2001
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9. Genome assembly reborn: recent computational challenges
Research into genome assembly algorithms has experienced a resurgence due to new challenges created by the development of next generation sequencing technologies. Several genome assemblers have been published in recent years specifically targeted at the new sequence data; however, the ever-changing technological landscape leads to the need for continued rese
Oxford University Press.
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10. Importance of anchor genomes for any plant genome project
Progress in agricultural and environmental technologies is hampered by a slower rate of gene discovery in plants than animals. The vast pool of genes in plants, however, will be an important resource for insertion of genes, via biotechnological procedures, into an array of plants, generating unique germ plasms not achievable by conventional breeding. It
The National Academy of Sciences.
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11. Identification of the Severe Acute Respiratory Syndrome Coronavirus by Simultaneous Multigene DNA Sequencing
The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the en
American Society for Microbiology.
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12. Gene prediction and verification in a compact genome with numerous small introns
The genomes of clusters of related eukaryotes are now being sequenced at an increasing rate, creating a need for accurate, low-cost annotation of exon–intron structures. In this paper, we demonstrate that reverse transcription-polymerase chain reaction (RT–PCR) and direct sequencing based on predicted gene structures satisfy this need, at least for singl
Cold Spring Harbor Laboratory Press.