Enzyme Stabilization
Mostrando 1-12 de 224 artigos, teses e dissertações.
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1. CASE SERIES OF PATIENTS UNDER BIWEEKLY TREATMENT WITH LARONIDASE: A REPORT OF A SINGLE CENTER EXPERIENCE
RESUMO Objetivo: Descrever a manutenção dos níveis de glicosaminoglicano (GAG) excretados na urina e da estabilização clínica em pacientes com mucopolissacaridose do tipo I (MPS I) com o uso da laronidase num regime de dose alternativo de 1,2 mg/kg a cada duas semanas. Método: Alguns pacientes do nosso serviço participaram de um estudo de otimiza
Rev. paul. pediatr.. Publicado em: 09/05/2019
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2. Stabilization of guava nectar with hydrocolloids and pectinases
Abstract The aim of this study was to stabilize guava nectar using hydrocolloids and/or enzymes, and evaluate the stability and the bioactive compounds content during storage. In general, there was a decrease in pH and an increase in titratable acidity and soluble solids of the nectars. During storage, it was observed that nectars with pectinase showed decr
Polímeros. Publicado em: 15/03/2018
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3. Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability
BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated th
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
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4. Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii
A lipase from the endophytic fungus Cercospora kikuchii was purified, biochemically characterized and the effects of spray drying on stabilization of the purified enzyme were studied. The lipase was purified 9.31-fold with recovery of 26.6% and specific activity of 223.6 U/mg. The optimum pH and temperature were 4.6 and 35 ºC, respectively, while the Vmax w
Braz. J. Chem. Eng.. Publicado em: 2014-12
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5. Enzyme replacement therapy for Mucopolysaccharidosis Type I among patients followed within the MPS Brazil Network
Mucopolysaccharidosis type I (MPS I) is a rare lysosomal disorder caused by deficiency of alph-L-iduronidase. Few clinical trials have assessed the effect of enzyme replacement therapy (ERT) for this condition. We conducted an exploratory, open-label, non-randomized, multicenter cohort study of patients with MPS I. Data were collected from questionnaires com
Genet. Mol. Biol.. Publicado em: 2014
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6. Target-directed catalytic metallodrugs
Most drugs function by binding reversibly to specific biological targets, and therapeutic effects generally require saturation of these targets. One means of decreasing required drug concentrations is incorporation of reactive metal centers that elicit irreversible modification of targets. A common approach has been the design of artificial proteases/nucleas
Braz J Med Biol Res. Publicado em: 02/07/2013
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7. Effect of silybin on high-fat-induced fatty liver in rats
Silybin, a natural antioxidant, has been traditionally used against a variety of liver ailments. To investigate its effect and the underlying mechanisms of action on non-alcoholic fatty liver in rats, we used 60 4-6-week-old male Sprague-Dawley rats to establish fatty liver models by feeding a high-fat diet for 6 weeks. Hepatic enzyme, serum lipid levels, ox
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-07
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8. Produção de agua de coco (cocus nucifera) utilizando membranas de micro e ultrafiltração de celulose, PES e PVDF / Coconut water processing using micro and ultrafiltration membranes of cellulose, PES and PVDF
Coconut water is a natural drink. Lately, its consumption has been growing in both natural form and processed form, because of the coconut water health benefits. Processing methods are used, essentially, to inhibit the enzymes actions (polyphenoloxidase and peroxidase) that are responsible for coconut water color chage to rose and ensure its microbiological
Publicado em: 2010
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9. Hidrólise do óleo da amêndoa da macaúba com lipase extracelular de "Colletotrichum gloesporioides" produzida por fermentação em substrato líquido
Extracellular lipase production by the filamentous fungi Colletotrichum gloesporioides was tested using two fermentation methods: in liquid substrate and in solid substrate. The liquid substrate fermentation yielded a larger production of lipase by this microorganism and was selected as the method for obtaining the extracellular lipase crude solution. Aliquo
Publicado em: 2010
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10. Caracterização bioquímica e secagem em "spray dryer" de lipases produzidas pelo fungo endofítico Cercospora kikuchii / Biochemical characterization and spray drying of lipases produced by the endophytic fungus Cercospora kicuchii.
Lipases são enzimas que catalisam a hidrólise de triacilgliceróis em ácidos graxos, mono e diacilgliceróis e glicerol. Em contraste com as esterases, lipases são ativadas apenas quando estão adsorvidas a uma interface óleo-água. Lipases têm sido amplamente utilizadas em muitos processos industriais, tais como química orgânica, formulações de de
Publicado em: 2010
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11. Flavins promote changes in the extracellular matrix, signal transduction, antioxidant enzymes and metalloproteinases during osteoblast differentiation / Flavinas promovem mudanças na matriz extracelular, vias de transdução de sinal, enzimas antioxidantes e metaloproteinases durante a diferenciação de osteoblastos
Riboflavin (Rb-Vitamin B2) is the precursor of essential flavocoenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). These coenzymes participate in numerous enzymatic processes dependent on electron transfer reactions that occur in energyproducing, biosynthetic, and detoxifying and electron-scavenging pathways. Increase dietary ribofla
Publicado em: 2009
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12. Imobilização e estabilização de D-Hidantoinase para a produção de N-Carbamoil-D-Fenilglicina
Immobilization and stabilization of enzymes increases their potential for use in industrial scale. D-hydantoinases (dihidropirimidina amidrohidrolase EC 3.5.2.2) catalyze the hydrolysis of D-hydantoins, generating the corresponding Ncarbamoil- D-amino acid and are used in the production of D-amino acids, including Dphenylglycine and D-p-hydroxyphenylglycine.
Publicado em: 2008