Enzymes Denaturation
Mostrando 1-12 de 75 artigos, teses e dissertações.
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1. Characterization of hydrolysates of collagen from mechanically separated chicken meat residue
Abstract Obtaining collagen and hydrolysates from mechanically separated chicken meat residue is an excellent way to add value to this waste. The aims of this study were to obtain collagen hydrolysates from chicken MSM residue using Alcalase® and Flavourzyme® enzymes, as well characterizing the functional, structural and thermal stability properties. The h
Food Sci. Technol. Publicado em: 2020-06
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2. Thermal inactivation of polyphenoloxidase and peroxidase in Jubileu clingstone peach and yeast isolated from its spoiled puree
The thermal inactivation of yeast isolated from spoiled Jubileu peach puree and that of polyphenoloxidase (PPO) and peroxidase (POD) in cv. Jubileu, which is widely cultivated in southern Rio Grande do Sul state, Brazil, were studied. PPO and POD were extracted using the protein powder method and submitted to partial purification by precipitation followed by
Food Sci. Technol. Publicado em: 2014-03
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3. Potencialidades do ácido 4-[(1E)etanohidrazonoil]benzóico como biomimético para a esterase da acetiltiocolina / Potencialities of [4-(1E)ethanehydrazonoyl]benzoic acid as a biomimetic for acetylthiocholine esterase
The application of pesticides and the resulting contamination have been a matter of constant concern, being necessary to monitor these compounds by methods that are able to quantify these compounds in water and food. The chromatographic techniques are most often used for this kind of analysis, but present some drawbacks for \"in situ\" or in real time applic
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/02/2012
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4. Nanostructures oxy-hydroxides of iron (III) stabilized by a polymeric matrix for use in cathodes of biomimetic fuel biocélulas / Nanoestruturas de óxi-hidróxidos de ferro (III) estabilizadas em matriz polimérica para aplicação em cátodos biomiméticos de biocélulas a combustível
Biofuel cells (BFCs) are electrochemical devices that converts a source fuel into an electric current in the presence of biocatalysts. In the case of enzyme BFCs, there are some limitations, such as high control of pH, denaturation of proteins at some conditions, high cost of enzymes, hard ways of bimolecular immobilization, and others. However, the developm
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 31/05/2011
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5. Antioxidant system of ginseng under stress by cadmium
Toxic levels of Cd can cause protein denaturation and oxidative stress, which result in membrane damage, enzimatic activity changes and other metabolic damage. Some plants may show alteration in the activity of their antioxidant enzymes as a heavy metal tolerance mechanism. This study aims at evaluating the role of enzymes of the antioxidant system in adapti
Scientia Agricola. Publicado em: 2011-08
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6. Availability of ca, cu, fe, mg and zn in bovine, pork and chicken meat samples raw and termically processed / Determinação da disponibilidade de Ca, Cu, Fe, Mg e Zn em amostras de carnes bovinas, suínas e de frango In natura e processadas termicamente
In this work, the availability of Ca, Cu, Fe, Mg, and Zn in bovine, pork and chicken meat samples raw and after thermal treatments: water boiled, grilled, microwave, convention oven I 180 oC (FCI) and conventional oven II 60 min and 180 oC was evaluated. Total determination of Ca, Cu, Fe, Mg and Zn in bovine and pork samples was performed by inductively coup
Publicado em: 2010
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7. Avaliação do efeito do sal volatil carbamato de amonio sobre a estabilidade de enzimas / Evaluation of the effect of ammonium carbamate on the stability of enzymes
Salting-out is a precipitation method frequently found in downstream processing schemes of proteins. However, the need for post-precipitation treatment for salt recovery and final purification of the protein may lead precipitation with conventional salts to be costly and limited for environmental questions. Precipitation with Volatile salts is an alternative
Publicado em: 2008
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8. Bromelain enzymatic activity in solutions at different temperatures and pH / Estudo da atividade enzimatica da bromelina pura em solução em diferentes temperaturas e pH
Bromelina é uma enzima de origem vegetal obtida de diversas espécies da família Bromeliaceae, presente na casca, no talo e no fruto do abacaxi. O Brasil encontra-se entre um dos maiores produtores mundiais de abacaxi, ocupando o terceiro lugar no ranking mundial. O desenvolvimento de novas técnicas de extração e purificação da bromelina vem sendo bem
Publicado em: 2007
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9. Extração e caracterização da fração lipolitica de residuos de processamento de mamão formosa. / Extration and characterization of the lipolítica fraction of residues of formosa processing of papaya.
Today, Brazil is the world largest papaya producer and the market of products derived from papaya is growing year after year. Consequently, the generation of byproducts, such as skin and seed, also increases. However, today there is no appropriate ways of wasting these byproducts. Based on this observation, we study the viability of using the byproducts of F
Publicado em: 2005
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10. Estudo da recuperação da enzima G6PDH, em colunas de campanulas pulsantes, com o uso de micelas reversas / Studies of recovery of G6PDH enzyme, in pulsed caps columns, using reversed micelles
The enzime glucose-6-phosphate dehydrogenase (G6PDH) it can be obtained of animal origin, vegetable or microbial, as in the case of the yeast Saccharomyces cerevisiae. This enzymes presents great importance for the cellular survival once it participates in the regulation of the cycle of the pentosys being responsible for the support of an appropriate level o
Publicado em: 2004
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11. Extração liquido-liquido de xilanase por micela reversa numa microcoluna de campanulas pulsadas
In this work, it was studied a microcolumn agitated by puIsed caps, due to promote an efficient contact between the phases in the column and also to avoid the enzyme denaturation and the loss of main proteins properties. This work deals with the purification of xylanase produced by Penicillium janthinellum by liquid-liquid extraction on reverse micelles, to
Publicado em: 2001
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12. Contribuição ao estudo da ação das enzimas coagulantes sobre a caseina acida e suas frações Alfa, Beta e K
Calf rennin and coagulating enzymes from Mucor miehei, Endothia parasitica were used in tests to compare their actions upon acid casein and its fractions α, β and K. The three coagulating enzymes showed distinct diffusion bands in casein-agar gel which indicated that they have different physical properties. Incubation of whole acid casein and its f
Publicado em: 1980