Enzymes Purification
Mostrando 1-12 de 718 artigos, teses e dissertações.
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1. Application and comparison of proteolytic enzyme preparations in technology of protein hydrolyzates
Abstract Protepsin belongs to the class of acid proteinases, containing carboxyl groups in the active center. The preparation cleaves peptide bonds formed by aromatic amino acids (tyrosine, phenylalanine), and does not hydrolize esters and amides. Along with the proteolytic complex, Protepsin contains collagenase, lipase, and other digestive enzymes. The pre
Food Sci. Technol. Publicado em: 2020-06
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2. Application and comparison of proteolytic enzyme preparations in technology of protein hydrolyzates
Abstract Protepsin belongs to the class of acid proteinases, containing carboxyl groups in the active center. The preparation cleaves peptide bonds formed by aromatic amino acids (tyrosine, phenylalanine), and does not hydrolize esters and amides. Along with the proteolytic complex, Protepsin contains collagenase, lipase, and other digestive enzymes. The pre
Food Sci. Technol. Publicado em: 28/11/2019
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3. DESIGN OF IMMOBILIZED ENZYME BIOCATALYSTS: DRAWBACKS AND OPPORTUNITIES
The enzymatic processes are increasingly highlights, especially in the synthesis of chemical products with high added value. The enzyme immobilization can improve industrial biocatalytic processes. The immobilization of enzymes provides the production of efficient, stable biocatalysts, possibility of reuse and easy purification of the products, when compared
Quím. Nova. Publicado em: 26/08/2019
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4. Produção e purificação integrada de protease fibrinolítica de Mucor subtilissimus UCP 1262
RESUMO As enzimas fibrinolíticas podem ser obtidas de micro-organismos por meio de processos fermentativos. O presente trabalho teve como objetivo avaliar a produção e extração integrada da protease fibrinolítica de Mucor subtilissimus UCP 1262 usando sistema de duas fases aquosas (SDFA). O processo integrado foi realizado para avaliar a produção, pa
Arq. Bras. Med. Vet. Zootec.. Publicado em: 06/06/2019
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5. Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Abstract Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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6. Protein PEGylation for the design of biobetters: from reaction to purification processes
The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and
Braz. J. Pharm. Sci.. Publicado em: 08/11/2018
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7. Collagenolytic enzymes produced by fungi: a systematic review
Abstract Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data b
Braz. J. Microbiol.. Publicado em: 2017-03
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8. Cholinesterase inhibitory activity and structure elucidation of a new phytol derivative and a new cinnamic acid ester from Pycnanthus angolensis
ABSTRACT The leaves of Pycnanthus angolensis (Welw.) Warb., Myristicaceae, are used as memory enhancer and anti-ageing in Nigerian ethnomedicine. This study aimed at evaluating the cholinesterase inhibitory property as well as isolates the bioactive compounds from the plant. The acetylcholinesterase and butyrylcholinesterase inhibitory potentials of extracts
Rev. bras. farmacogn.. Publicado em: 2016-08
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9. Bromelain: Methods of Extraction, Purification and Therapeutic Applications
Bromelain is a concoction of sulfhydryl proteolytic enzymes. Depending upon the site of extraction it can be regarded as either stem bromelain (SBM) (EC 3.4.22.32) or fruit bromelain (FBM) (EC 3.4.22.33). Bromelain remain enzymatic active over a broad spectrumand endure a range of pH (5.5 to 8.0) and temperature (35.5 to 71 ºC). It is one of the extensively
Braz. arch. biol. technol.. Publicado em: 06/05/2016
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10. Gene cloning, expression, and characterization of the Bacillus amyloliquefaciens PS35 lipase
Abstract Lipases are enzymes of immense industrial relevance, and, therefore, are being intensely investigated. In an attempt to characterize lipases at molecular level from novel sources, a lipase gene from Bacillus amyloliquefaciens PS35 was cloned, heterologously expressed in Escherichia coli DH5α cells and sequenced. It showed up to 98% homology with ot
Braz. J. Microbiol.. Publicado em: 09/10/2015
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11. Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specim
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
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12. Thermal inactivation of polyphenoloxidase and peroxidase in Jubileu clingstone peach and yeast isolated from its spoiled puree
The thermal inactivation of yeast isolated from spoiled Jubileu peach puree and that of polyphenoloxidase (PPO) and peroxidase (POD) in cv. Jubileu, which is widely cultivated in southern Rio Grande do Sul state, Brazil, were studied. PPO and POD were extracted using the protein powder method and submitted to partial purification by precipitation followed by
Food Sci. Technol. Publicado em: 2014-03