Epsilon Prototoxin
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. "Extração da pró-toxina épsilon e de uma protease a partir de ´Clostridium perfringens´ em sistemas de duas fases aquosas utilizando PEG/citrato" / Extraction of epsilon prototoxin and protease from Clostridium perfringens by aqueous two-phase systems using PEG/Citrate
Este trabalho tem como finalidade obter condições de recuperar e purificar a pró-toxina épsilon e uma protease produzida pelo Clostridium perfringens através do uso da técnica de extração líquido-líquido em sistema de duas fases aquosas (SDFA) para utilização na produção de vacinas. A aplicação do sistema de duas fases aquosas é proposta com
Publicado em: 2004
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2. Tryptophan content of Clostridium perfringens epsilon toxin.
The tryptophan content of Clostridium perfringens epsilon toxin was investigated. When the tryptophan content was determined by amino acid analysis after the hydrolysis of epsilon prototoxin with methanesulfonic acid containing 3-(2-aminoethyl)indole and by the spectrophotometric method with N-bromosuccinimide, the number of tryptophan residues was calculate
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3. Physical changes in the epsilon prototoxin molecule of Clostridium perfringens during enzymatic activation.
Enzymatic activation of Clostridium perfringens epsilon prototoxin removed a small basic part of the molecule, causing a slight change in molecular weight (32,700 to 31,200) and a large change in isoelectric point (from pH 8.02 into fractions of 5.36 and 5.74).
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4. Effect of Clostridium perfringens epsilon toxin on the cardiovascular system of rats.
Pressor activity was demonstrated by the intravenous injection of purified epsilon toxin of Clostridium perfringens type D but not by that of epsilon prototoxin. The activity was completely abolished by C. perfringens type B or D antiserum, but not by type A, C, or E antiserum. The rise in blood pressure caused by the toxin was accompanied by a decrease in t