Escherichiacoli
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Highly Pathogenic and Multidrug Resistant Avian Pathogenic Escherichia Coli in Free-Range Chickens from Brazil
ABSTRACT Avian pathogenic Escherichiacoli (APEC) virulence mechanism has been continuously studied and it is believed to be multifactorial and because of this, this work aimed to characterize potentially APEC strains isolated from free-range hens. Isolates were submitted to PCR for the detection of virulence genes, which were of high prevalence. In vivo inoc
Braz. J. Poult. Sci.. Publicado em: 09/05/2019
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2. Efeito do sobrenadante de isolados de Lactobacillus sp. sobre Escherichia coli O157:H7 reforça o papel da produção de ácidos orgânicos como fator de controle patogênico
Muitas tentativas têm sido feitas para se estabelecer o controle de patógenos de origem alimentar através do uso de estirpes de Lactobacillus e dos seus produtos de metabolismo, com sucesso sendo sucedido em várias situações. O objetivo deste trabalho foi investigar o efeito antagônico do sobrenadante de culturas de oito isolados de Lactobacillus, inc
Pesq. Vet. Bras.. Publicado em: 2015-04
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3. Avaliação da qualidade da água e autodepuração do Rio Jordão, Araguari (MG) / Water quality evaluation and self-depuration of Jordão River, Araguari (MG)
Este trabalho avaliou a capacidade de autodepuração do rio Jordão considerando-se as contribuições reais do córrego Brejo Alegre em período de estiagem e chuvoso, tendo em vista o lançamento de efluentes brutos de parte da cidade de Araguari, Minas Gerais, no córrego, utilizando o modelo QUAL-UFMG. Também simulou-se dois cenários, considerando car
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/08/2011
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4. Role of the Xf-0818 cellulase in the Xylella fastidiosa X citrus interaction. / Papel da celulase XF-0818 na interação Xylella fastidiosa X Citros.
From the genome sequencing of the bacteria Xylella fastidiosa, the agent responsible for the citrus variegated chlorosis (CVC), studies on the pathogenicity mechanisms expressed during the occurrence of the disease were started in genomic function projects, aiming to confirm the function of the genic sequences identified in the project X. fastidiosa Genome.
Publicado em: 2005
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5. Effects of tetrodotoxin and ion replacements on the short-circuit current induced by Escherichiacoli heat stable enterotoxin across small intestine of the gerbil (Gerbillus cheesmani)
The effects of mucosally added Escherichia coli heat stable enterotoxin (STa 30 ng ml-1) on the basal short-circuit current (Isc in µA cm-2) across stripped and unstripped sheets of jejuna and ilea taken from fed, starved (4 days, water ad lib) and undernourished (50% control food intake for 21 days) gerbil (Gerbillus cheesmani) were investigated. The effec
Memórias do Instituto Oswaldo Cruz. Publicado em: 2004-03
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6. Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis*
An enzyme, which is probably identical with polynucleotide phosphorylase, was prepared from Escherichiacoli B. In the presence of Mn2+ it catalyzes the addition of one (and to a slight extent more) residue of deoxyribonucleotide residue from the diphosphate to an oligodeoxyribonucleotide primer. The shortest effective primers contained three phosphate residu
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7. Terminal oxidation-reduction of N-acetyl-phenylalanyl-tRNA blocks initiation complex formation with Escherichia coli 30S ribosomal subunits
Terminally oxidized-reduced tRNAPhe of yeast, exclusively acylated at the 2′-hydroxyl of the 3′-terminal ribose of the tRNA, is a useful model for investigating the stereospecificity of AA-tRNA in protein synthesis. In this work, the ability of N-acetyl-Phe-tRNAox-red to form an initiation complex with Escherichiacoli 30S ribosomal subunits was investiga
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8. RNA-linked nascent DNA pieces in phage T7-infected Escherichia coli. III. Detection of intact primer RNA
RNA-linked DNA fragments of T7-infected Escherichiacoli were labeled with [32P]orthophosphate invivo. The RNA segments of the labeled fragments were isolated by degrading the DNA portion with the 3′→ 5′ exonuclease intrinsic to bacteriophage T4 DNA polymerase and fractionated according to net charge by a DEAE-Sephadex A-25 column chromatography in the
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9. Exploring intracellular space: function of the Min system in round-shaped Escherichia coli
The MinCDE proteins help to select cell division sites in normal cylindrical Escherichia coli by oscillating along the long axis, preventing unwanted polar divisions. To determine how the Min system might function in cells with multiple potential division planes, we investigated its role in a round-cell rodA mutant. Round cells lacking MinCDE were viable, bu
Oxford University Press.
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10. Cloning, purification and bioactivity assay of human CD28 single-chain antibody in Escherichiacoli
Engineered single chain antibodies have become a powerful source of immunotherapy against a wide range of diseases. Here, we present the generation of human CD28 single-chain antibody gene (CD28-ScFv), which contained variable fragments of heavy chain and light chain (VH and VL) of the anti-CD28 antibody, and a linking peptide (Gly4Ser)3 inserted in the midd
Springer Netherlands.
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11. Quantitation of the common components of deoxyribonucleic acids by mass spectrometry: application to the analysis of DNAs of unusual composition
A mass spectral method for the quantitation of the percentages of deoxyadenosine, deoxyguanosine, deoxycytidine, and thymidine in intact DNAs has been devised. Standard curves for each nucleoside have been constructed which are based upon the observation that a direct correlation exists between the heights (% deflection) of diagnostic peaks from these nucleo
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12. Improvement of Posttranslational Bottlenecks in the Production of Penicillin Amidase in Recombinant Escherichiacoli Strains
Using periplasmic penicillin amidase (PA) from Escherichia coli ATCC 11105 as a model recombinant protein, we reviewed the posttranslational bottlenecks in its overexpression and undertook attempts to enhance its production in different recombinant E. coli expression hosts. Intracellular proteolytic degradation of the newly synthesized PA precursor and trans
American Society for Microbiology.