Faecal Dna
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. INVESTIGATION OF ENTEROCOCCUS FAECALIS POPULATION IN PATIENTS WITH POLYP AND COLORECTAL CANCER IN COMPARISON OF HEALTHY INDIVIDUALS
RESUMO CONTEXTO: O câncer colorretal é um dos cânceres mais comumente diagnosticados em todo o mundo. Um dos fatores envolvidos no desenvolvimento do câncer colorretal é a mudança na flora normal do intestino. OBJETIVO: O número médio de cópias de Enterococcus faecalis em pessoas com pólipos e pessoas com câncer colorretal foram avaliados em c
Arq. Gastroenterol.. Publicado em: 26/08/2019
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2. Detection of Plasmodium in faeces of the New World primate Alouatta clamitans
Abstract Plasmodium falciparum and Plasmodium vivax have evolved with host switches between non-human primates (NHPs) and humans. Studies on the infection dynamics of Plasmodium species in NHPs will improve our understanding of the evolution of these parasites; however, such studies are hampered by the difficulty of handling animals in the field. The aim of
Mem. Inst. Oswaldo Cruz. Publicado em: 25/08/2016
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3. Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil
To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-09
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4. Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes
A real-time polymerase chain reaction (PCR) assay with fluorescence resonance energy transfer (FRET) hybridisation probes combined with melting curve analysis was developed to detect Schistosoma japonicum in experimentally infected snails and in faecal samples of infected mice. This procedure is based on melting curve analysis of a hybrid between an amplicon
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
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5. Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization
Brazilian Journal of Microbiology. Publicado em: 2011-03
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6. Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats
More sensitive methodologies are necessary to improve strongyloidiasis diagnosis. This study compared the sensitivities of the McMaster modified technique and polymerase chain reaction (PCR) assays, both performed in faecal samples. Lewis rats were subcutaneously infected with 4,000, 400 or 40 infective third-stage larvae, considered as high, moderate or low
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-02
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7. Análise molecular de amostras fecais de uma população de veado-mateiro (Mazama americana) para a obtenção de informações genéticas e ecológicas / Molecular analysis of faecal samples of a red brocket deer (Mazama americana) population for obtaining genetic and ecological information
Mazama genus is composed by five species in Brazil. All of them are difficult to observe due to their evasive behaviors, what makes the captures and behavioral studies almost impossible. Thus, the use of non invasive methodologies is necessary to study the ecology and genetics of these species. The fecal DNA analysis is one of the most promising techniques f
Publicado em: 2010
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8. Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-12
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9. "Padronização e avaliação de métodos moleculares para detecção de oocistos de Cryptosporidium spp. (Apicomplexa: Cryptosporiidae) em amostras fecais: extração de DNA genômico e PCR (reação em cadeia pela polimerase)" / Standardization and evaluation of molecular methods to detect oocysts of Cryptosporidium spp (Apicomplexa: Cryptosporiidae) in faecal samples: extraction of genomic DNA and PCR (polymerase chain reaction).
O protozoário parasita Cryptosporidium parvum tem sido reconhecido como um importante patógeno emergente. Para estudos moleculares, a maioria das técnicas para extração do DNA requer o uso de kits importados para concentrar, romper a parede muito resistente do oocisto e purificar o DNA das matrizes das amostras. O objetivo do estudo foi desenvolver um m
Publicado em: 2004
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10. Antibodies to Escherichia coli O157 in patients with haemorrhagic colitis and haemolytic uraemic syndrome.
Twenty four sera from patients with haemolytic uraemic syndrome or haemorrhagic colitis and healthy controls were examined for antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157. Faecal specimens from these patients were also examined for Vero cytotoxin producing E coli (VTEC) by DNA probes, and for faecal Vero cytotoxin. Eight patients with
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11. The use of serodiagnosis in the retrospective investigation of a nursery outbreak associated with Escherichia coli O157:H7.
AIMS: To use serology to investigate an outbreak of verocytotoxin (VT) producing Escherichia coli O157 in a hospital nursery, following the detection of faecal E coli O157 (phage type 49) producing VT type 2. METHODS: ELISA and immunoblotting techniques, based on lipopolysaccharide (LPS) purified from E coli O157; diagnostic bacteriology; serotyping and phag