Fatty Acid Synthetase Complex
Mostrando 1-12 de 20 artigos, teses e dissertações.
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1. Avaliação do efeito do tratamento com Orlistat sobre a resposta imune contra melanomas experimentais / Analysis of the effects of Orlistat on the immune response against experimental melanomas
A enzima ácido graxo sintase (FASN), responsável pela síntese endógena de ácidos graxos, está presente em grande quantidade em diversas neoplasias malignas e lesões pré-malignas. Sua inibição farmacológica parece estar relacionada com a morte celular seletiva de células tumorais. Orlistat (Xenical®), uma droga anti-obesidade, que possui também
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/02/2012
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2. Avaliação da morte celular induzida por inibidores da enzima acido graxo sintase em linhagem celular derivada de melanoblastos não tumorigenicos de camundongos / Non-tumorigenic melanocyte cell death induced by fatty acid synthase inhibitors
Ácido graxo sintase (FASN - EC 2.3.1.85) é a enzima responsável pela síntese endógena de ácidos graxos de cadeia longa a partir dos precursores acetil-CoA e malonil-CoA. Diversos estudos mostram que a FASN é altamente expressa em vários tipos de neoplasias malignas humanas, tais como de próstata, mama, melanoma e, em alguns destes tumores, a alta ex
Publicado em: 2010
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3. A Saccharomyces cerevisiae Mutant Defective in Saturated Fatty Acid Biosynthesis*
The isolation and biochemical characterization of a Saccharomyces cerevisiae mutant, which grows only when emulsified myristic, palmitic, stearic, or oleic acid is added to the growth medium, is described. The mutant contains an enzymatically inactive fatty acid synthetase complex. The gene affected, preliminarily designated by the symbol fas, exhibits a 2:2
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4. Fatty acid synthetase from Brevibacterium ammoniagenes: formation of monounsaturated fatty acids by a multienzyme complex.
A multienzyme fatty acid synthetase complex isolated from Brevibacterium ammoniagenes has been purified to a specific activity of 1440 nmol of malonyl-CoA incorporated per min/mg. The enzyme is homogeneous, as judged by gel electrophoresis on agarose gels, and has a molecular weight of 1.2 X 10(6). Both NADPH and NADH are required for activity. In contrast t
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5. Specific release of the thioesterase component of the fatty acid synthetase multienzyme complex by limited trypsinization.
Limited trypsinization of the fatty acid synthetase multienzyme complex from rat mammary gland results in the release of a protein, molecular weight 32,000, with thioesterase activity. The other components of the multienzyme complex--the acyl carrier protein, acetyl and malonyl transferases, condensing enzyme, keto reductase, dehydrase and enoyl reductase--a
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6. CRYSTALLIZATION OF A MULTIENZYME COMPLEX: FATTY ACID SYNTHETASE FROM YEAST
The crystallization of purified fatty acid synthetase from yeast is facilitated by seeding techniques. The first seed crystals appeared as particles with almost negligible enzymatic activity in a solution that had been left at 4°C for 15 months. Subsequently, the crystallization time has been reduced to two days, and the crystals now isolated have retained
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7. Purification and properties of the fatty acids synthetase complex from Neurospora crassa, and the nature of the fas-mutation.
A procedure is described for the purification of the fatty acid synthetase complex (FAS) from Neurospora crassa. The enzyme complex has a molecular weight of 2.3 times 10(6), contains 6 mol of 4'-phosphopantetheine per mol, and on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate gives a single band, or a closely spaced doublet, wh
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8. Incorporation of C14-pantothenate into the fatty acid synthetase complex of growing baker's yeast.
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9. Induction of acyl coenzyme A synthetase and hydroxyacyl coenzyme A dehydrogenase during fatty acid degradation in Neurospora crassa.
Neurospora crassa is able to use long-chain fatty acids as the sole carbon and energy source. After growth on oleate there was nearly a 10-fold induction of the acyl coenzyme A (CoA) synthetase and a fivefold increase in the activity of the 3-hydroxyacyl-CoA dehydrogenase. There was a slight induction of the enoyl-CoA hydratase and 3-ketoacyl-CoA thiolase, b
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10. Molecular Characterization of an Arabidopsis Acyl-Coenzyme A Synthetase Localized on Glyoxysomal Membranes1
In higher plants, fat-storing seeds utilize storage lipids as a source of energy during germination. To enter the β-oxidation pathway, fatty acids need to be activated to acyl-coenzyme As (CoAs) by the enzyme acyl-CoA synthetase (ACS; EC 6.2.1.3). Here, we report the characterization of an Arabidopsis cDNA clone encoding for a glyoxysomal acyl-CoA synthetas
American Society of Plant Biologists.
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11. Fatty Acid Synthetase Activity in Mycobacterium phlei: Regulation by Polysaccharides
The multienzyme complex from Mycobacterium phlei which catalyzes the synthesis of long chain fatty acids from acetyl-CoA and malonyl-CoA requires a heat-stable fraction (stimulating factor, SF) for activity. Fractionation of heat-treated M. phlei extracts affords two stimulatory subfractions, one of which (SF2) can be replaced by FMN. The other (SF1) is furt
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12. Genetic Analysis of Hybrid Strains Trisomic for the Chromosome Containing a Fatty Acid Synthetase Gene Complex (fas1) in Yeast
Evidence of spontaneous n+1 aneuploidy has been obtained by trisomic segregation analysis of four independently maintained stocks of Saccharomyces cerevisiae defective in saturated fatty acid synthesis (fas1). In all cases tested, only the chromosome bearing the mutant fatty acid locus was disomic. Tetrad analysis of trisomic hybrids enabled the identificati