Fetal Bovine Serum Fbs
Mostrando 1-12 de 21 artigos, teses e dissertações.
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1. Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth
Stem cells from human exfoliated deciduous teeth (SHEDs) have great potential to treat various dental-related diseases in regenerative medicine. They are usually maintained with 10% fetal bovine serum (FBS) in vitro. Modified platelet-rich plasma (mPRP) would be a safe alternative to 10% FBS during SHEDs culture. Therefore, our study aimed to compare the pro
Braz J Med Biol Res. Publicado em: 01/09/2016
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2. Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency
Abstract Dental trauma in immature permanent teeth can damage pulp vascularization, which leads to necrosis and cessation of apexogenesis. Studies on tissue engineering using stem cells from human exfoliated deciduous teeth (SHEDs) have yielded promising results. Laser phototherapy (LPT) is able to influence the proliferation and differentiation of these cel
Braz. oral res.. Publicado em: 31/05/2016
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3. Modelos para a produção de eritropoietina recombinante humana in vivo e in vitro com vetores plasmideais em ovinos / Models for the production of human recombinant erythropoietin in vivo and in vitro with plasmidial vectors in ovine
Some post-translational modifications are necessary for the production of biopharmaceutical proteins, such as recombinant human erythropoietin (rhEPO), with a good specific action and a high biological activity. These modifications are obtained only by bioreactors based on eukaryotic cell as mammary cells. Bioreactors, in vivo or in vitro, with this kind of
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/02/2011
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4. Hypothyroidism decreases proinsulin gene expression and the attachment of its mRNA and eEF1A protein to the actin cytoskeleton of INS-1E cells
The actions of thyroid hormone (TH) on pancreatic beta cells have not been thoroughly explored, with current knowledge being limited to the modulation of insulin secretion in response to glucose, and beta cell viability by regulation of pro-mitotic and pro-apoptotic factors. Therefore, the effects of TH on proinsulin gene expression are not known. This led u
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-10
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5. Nanobacteria-like particles : a threat to cell cultures
The main goal of this study is to alert researchers who work with cell cultures for the risk of contamination by structures called nanobacteria (NB). NB are tiny structures with size varying from 80 to 500 nm, commonly occurring in clusters and producing a biofilm which contains carbonate or hydroxyl apatite. The most likely source of cell culture contaminat
Publicado em: 2010
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6. Caracterização metabólica e cinética do cultivo de três hibridomas para produção de imunoglobulinas com especificidade e antígenos eritrocitários para uso hemoterápico.
In the last two decades mammalian cell cultures have been broadly used for the industrial production of pharmaceutical, proteins, in particular the monoclonal antibodies by cultures of murine hybrid cells (hybridoma). The development of bioprocess for industrial scale of monoclonal antibodys (MAbs) depends on the determination and optimization of several phy
Publicado em: 2007
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7. Influência do fracionamento da energia de irradiação na fototerapia com laser em baixa intensidade sobre o crescimento de fibroblastos de polpa dentária humana / Influence of the fractioned irradiation energy in the phototherapy with low intensity laser on the growth of human dental pulp fibroblasts
Phototherapy with low intensity lasers has been used in dentistry in several oral pathologies for pain and healing control. The aim of this study was to compare the effect of phototherapy on human dental pulp fibroblasts growth using irradiations with whole energy delivered at once or fractioned. After the determination of the methodology, the FP5 cell line
Publicado em: 2007
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8. Influência da terapia laser de baixa intencidade em células precursoras miogênicas (in vitro) e durante a regeneração muscular (in vivo).
The Low Intensity Laser Therapy (LILT) constitutes one of the resources most used by physical therapists, with the goal of stimulating the process of repairing the skeleton muscle tissue. On the other hand, the intense utilization, its respective action mechanisms and therapeutic effectiveness are not scientifically proved. Based on this, we elaborated the p
Publicado em: 2004
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9. Comparison of a serum replacement (Omni Serum) and fetal bovine serum in cell cultures used to isolate herpes simplex virus from clinical specimens.
Traditionally, fetal bovine serum (FBS) has been the principal component in media used in the growth and maintenance of cell cultures. Recent shortages have affected the cost and availability of FBS to clinical laboratories. Furthermore, lot-to-lot variability can affect cell culture performance and growth. We evaluated a commercially available serum replace
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10. Activity in fetal bovine serum that stimulates erythroid colony formation in fetal mouse livers is insulinlike growth factor I.
In the present study, the erythropoietic activity of fetal serum was characterized. Using fetal bovine serum (FBS) as a source of the erythropoietic activity and serum-free cultures of fetal mouse livers (FMLC assay) as a detection system, we found that FBS stimulated colony formation from late erythroid progenitor cells (CFU-E) in a dose-dependent fashion.
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11. Fortified Sera and Their Use in Environmental Virology
Four commercially available fortified sera were compared to fetal bovine serum (FBS) with regard to their ability to maintain or increase the sensitivity of the Buffalo green monkey (BGM) kidney cell line to viral infection. Nine virus strains and five wastewater samples were used. Fortified sera were comparable to FBS for the enumeration of some viruses by
American Society for Microbiology.
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12. Neutral Red Assay for Measurement of Quantitative Vero Cell Cytotoxicity
A neutral red assay involving Vero cells was used to quantitate the cytotoxic activity of verotoxins (VT) produced by Escherichia coli and to investigate changes in titer caused by altering the composition of the cell culture medium. Three variations on medium 199 were investigated: one involved supplementing the medium with 5% fetal bovine serum (FBS), a se