Fidelity Of Replication
Mostrando 1-12 de 167 artigos, teses e dissertações.
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1. Moldagem por injeção de microcomponentes ópticos poliméricos gerados em insertos usinados por torneamento de ultraprecisão / Injection molding of polymer micro-optical components generated in inserts by ultra-precision turning
Este trabalho analisou o processo de moldagem por injeção de micro elementos ópticos difrativos usinados em insertos de cobre eletrolítico com ferramenta de diamante com ponta única. Quatro tipos de microestruturas características foram selecionados neste estudo, a saber: lente anesférica, lente de Fresnel, grade de difração (blaze grating) e sensor
Publicado em: 2010
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2. Characterization of Brazilian isolates of equine herpesvirus type A / Caracterização genomica de isolados brasileiros do herpesvirus equino do tipo 1
Equine Herpesvirus Type 1 (EHV-1) is a member of Alphaherpesvirinae subfamily implicated with abortions, respiratory and neurological disturbs in horses. The principal mode of viral transmission is through close contact virus-containing secretions of infected horses. Systemic pathogenesis in which this virus is implicated combines primary respiratory infecti
Publicado em: 2005
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3. Fidelity of a human cell DNA replication complex.
We have measured the fidelity of bidirectional, semiconservative DNA synthesis by a human DNA replication complex in vitro. Replication was performed by extracts of HeLa cells in the presence of simian virus 40 (SV40) large tumor antigen by using a double-stranded phage M13mp2 DNA template containing the SV40 origin of replication and either of two different
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4. Fidelity of DNA synthesis in a mammalian in vitro replication system.
We have used the simian virus 40 (SV40)-based shuttle vector pZ189 in a forward-mutation assay to determine the fidelity of DNA replication in the in vitro DNA replication system developed by J.J. Li and T.J. Kelly (Proc. Natl. Acad. Sci. USA 81:6973-6977, 1984). We find that very few base substitution errors (approximately 1/180,000 bases incorporated) are
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5. The fidelity of the human leading and lagging strand DNA replication apparatus with 8-oxodeoxyguanosine triphosphate.
A product of oxidative metabolism, 8-oxodeoxyguanosine triphosphate (8-O-dGTP), readily pairs with adenine during DNA replication, ultimately causing A.T-->C.G transversions. This study utilized 8-O-dGTP as a probe to examine the fidelity of the leading and lagging strand replication apparatus in extracts of HeLa cells. Simian virus (SV) 40 T antigen-depende
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6. Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome
We have investigated the question whether during chromosomal DNA replication in Escherichia coli the two DNA strands may be replicated with differential accuracy. This possibility of differential replication fidelity arises from the distinct modes of replication in the two strands, one strand (the leading strand) being synthesized continuously, the other (th
The National Academy of Sciences.
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7. High-fidelity in vivo replication of DNA base shape mimics without Watson–Crick hydrogen bonds
We report studies testing the importance of Watson–Crick hydrogen bonding, base-pair geometry, and steric effects during DNA replication in living bacterial cells. Nonpolar DNA base shape mimics of thymine and adenine (abbreviated F and Q, respectively) were introduced into Escherichia coli by insertion into a phage genome followed by transfection of the v
The National Academy of Sciences.
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8. Exonucleolytic proofreading of leading and lagging strand DNA replication errors.
We have asked whether exonucleolytic proofreading occurs during simian virus 40 origin-dependent, bidirectional DNA replication in extracts of human HeLa cells. In addition, we have compared the fidelity of leading and lagging strand DNA synthesis. In a fidelity assay that scores single-base substitution errors that revert a TGA codon in the lacZ alpha gene
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9. The cost of replication fidelity in an RNA virus
It is often argued that high mutation rates are advantageous for RNA viruses, because they confer elevated rates of adaptation. However, there is no direct evidence showing a positive correlation between mutation and adaptation rates among RNA viruses. Moreover, theoretical work does not argue in favor of this prediction. We used a series of vesicular stomat
National Academy of Sciences.
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10. Mutants of Escherichia Coli with Increased Fidelity of DNA Replication
To improve our understanding of the role of DNA replication fidelity in mutagenesis, we undertook a search for Escherichia coli antimutator strains with increased fidelity of DNA replication. The region between 4 and 5 min of the E. coli chromosome was mutagenized using localized mutagenesis mediated by bacteriophage P1. This region contains the dnaE and dna
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11. Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment
The primary structures of the replicative DNA polymerases (gp43s) of bacteriophage T4 and its distant phylogenetic relative RB69 are diverged, retaining only 61% identity and 74% similarity. Nevertheless, RB69 gp43 substitutes effectively for T4 gp43 in T4 DNA replication in vivo. We show here that RB69 gp43 replicates T4 genomes in vivo with a fidelity simi
The National Academy of Sciences of the USA.
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12. Fidelity of replication of the leading and the lagging DNA strands opposite N-methyl-N-nitrosourea-induced DNA damage in human cells.
Semi-conservative replication of double-stranded DNA in eukaryotic cells is an asymmetric process involving leading and lagging strand synthesis and different DNA polymerases. We report a study to analyze the effect of these asymmetries when the replication machinery encounters alkylation-induced DNA adducts. The model system is an EBV-derived shuttle vector