Fire Blight
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Fisiologia de Bacillus subtilis R14 sob condiÃÃes restrita e irrestrita de oxigÃnio: produÃÃo de compostos bioativos e esporulaÃÃo
Several strains of Bacillus subtilis produce lipopeptides, which are amphiphilic compounds with surfactant and antimicrobial activities. Production of lipopeptide, sporulation and competence in B. subtilis are interconnected through a complex array of controlling elements. The aims of this study were to evaluate the activity of B. subtilis R14 against severa
Publicado em: 2005
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2. Controle biológico de fire blight em pereiras empregando uma formulação de Pantoea agglomerans Eh 24
Controle biológico de Erwinia amylovora através do uso de bactérias epifíticas tem sido considerado um método alternativo para o controle de "fire blight". Uma formulação de Pantoea agglomerans Eh 24 em talco foi utilizada em pereiras a 30% e a 100% de floração, em duas plantações selecionadas na região Aegean da Turquia. Os experimentos foram re
Brazilian Journal of Microbiology. Publicado em: 2004-09
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3. Role of antibiotic production by Erwinia herbicola Eh252 in biological control of Erwinia amylovora.
Erwinia herbicola Eh252 is a nonpathogenic epiphytic bacterium that reduces fire blight incidence when sprayed onto apple blossoms before inoculation with Erwinia amylovora, the causal agent of fire blight. Eh252 was found to produce on minimal medium an antibiotic that inhibited the growth of E. amylovora. This antibiotic was inactivated by histidine but no
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4. Identification of the fire blight pathogen, Erwinia amylovora, by PCR assays with chromosomal DNA.
Erwinia amylovora, the causative agent of fire blight, was identified independently from the common plasmid pEA29 by three different PCR assays with chromosomal DNA. PCR with two primers was performed with isolated DNA and with whole cells, which were directly added to the assay mixture. The oligonucleotide primers were derived from the ams region, and the P
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5. Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora, the Fire Blight Pathogen
Serratia plymithicum J7 culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora, the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens, and Pseudomonas fluorescens. This activity increased significantly upon
American Society for Microbiology.
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6. Effect of Increased β-Glucosidase Activity on Virulence of Erwinia amylovora
Plant tissues often contain β-glucosides that can be enzymatically hydrolyzed to produce toxic aglycones. It has been suggested that the low β-glucosidase activity found in Erwinia amylovora contributes to bacterial virulence by allowing the bacteria to infect plants that contain β-glucosides without inducing the formation of toxic aglycones. To test this
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7. Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis.
Detection and identification of the fire blight pathogen, Erwinia amylovora, can be accurately done by polymerase chain reaction (PCR) analysis in less than 6 h. Two oligomers derived from a 29-kb plasmid which is common to all strains of E. amylovora were used to amplify a 0.9-kb fragment of the plasmid. By separation of the PCR products on agarose gel, thi
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8. Deoxyribonucleic Acid Relatedness Among Species of Erwinia and Between Erwinia Species and Other Enterobacteria
Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of related nucleotide sequences. An apparent
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9. Bacteriophages of Erwinia amylovora
Fifty bacteriophage isolates of Erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the Niagara region of southern Ontario and the Royal Botanical Gardens, Hamilton, Ontario. Forty-two phages survived the isolation, purification, and storage processes. The majority of the phages in the collection were isolated from the
American Society for Microbiology.
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10. Characterization of the rcsB gene from Erwinia amylovora and its influence on exoploysaccharide synthesis and virulence of the fire blight pathogen.
RcsB belongs to a family of positive regulators of exopolysaccharide synthesis in various enterobacteria. The rcsB gene of the fire blight pathogen Erwinia amylovora was cloned by PCR amplification with consensus primers, and its role in exopolysaccharide (EPS) synthesis was investigated. Its overexpression from high-copy-number plasmids stimulated the synth
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11. Molecular Analysis of Sucrose Metabolism of Erwinia amylovora and Influence on Bacterial Virulence
Sucrose is an important storage and transport sugar of plants and an energy source for many phytopathogenic bacteria. To analyze regulation and biochemistry of sucrose metabolism of the fire blight pathogen Erwinia amylovora, a chromosomal fragment which enabled Escherichia coli to utilize sucrose as sole carbon source was cloned. By transposon mutagenesis,
American Society for Microbiology.
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12. Isolation and Characterization of Five Erwinia amylovora Bacteriophages and Assessment of Phage Resistance in Strains of Erwinia amylovora
Phages able to infect the fire blight pathogen Erwinia amylovora were isolated from apple, pear, and raspberry tissues and from soil samples collected at sites displaying fire blight symptoms. Among a collection of 50 phage isolates, 5 distinct phages, including relatives of the previously described phages φEa1 and φEa7 and 3 novel phages named φEa100, φ
American Society for Microbiology.