Fluorescent Decay Time
Mostrando 1-12 de 30 artigos, teses e dissertações.
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1. Caracterização fisiológica e genética do transporte de arginina em Leishmania (Leishmania) amazonensis / Physiologic and genetic characterization of arginine transport in Leishmania (Leishmania) amazonensis
Protozoan of genus Leishmania are digenetic parasites that present a stage in the life in insect gut (promastigotes) and an intracellular phase (amastigotes) inside vertebrate host macrophages. The study of L-arginine influx consists in an interesting matter, since the amino acid is used on NO production pathway (the main macrophage microbial pathway) but ar
Publicado em: 2011
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2. EFFECT OF AQUEOUS FRACTION OF LEAVES DE COSTUS SPIRALIS (JACQ.) ROSCOE CONTRACTILE FUNCTION ON HEART MAMMALS. / EFEITO DA FRAÇÃO AQUOSA DAS FOLHAS DE COSTUS SPIRALIS (JACQ.) ROSCOE SOBRE A FUNÇÃO CONTRÁTIL DO CORAÇÃO DE MAMÍFEROS.
Teas and infusions from C. spiralis leaf have largely been used by folk medicine as diuretic, hypotensor, cytotoxic, immunomodulator, antilithiasic, antidiarrheic, antispasmodic, antiurolitic, antimicrobian, antifungic, antioxidant, antileishmania activity, antiinflamatory, and antiedematogenic activity. In spite of these biological effects attributed to the
Publicado em: 2011
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3. Nonlinear optical properties of aniline oligomers / Propriedades ópticas não-lineares de oligômeros de anilina
We report on the study of electronic optical non linearities in two aniline oligomers: dimer and the tetramer. Four tetramer concentrations were measured, pure and also 33 and 100% doped; one of dimer non doped another 100% doped. The solutions were prepared using dimethyl sulfoxide (DMSO) as solvent and the doping was performed with hydrochloric acid. The s
Publicado em: 2002
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4. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays
Much of the information available about factors that affect mRNA decay in Escherichia coli, and by inference in other bacteria, has been gleaned from study of less than 25 of the ≈4,300 predicted E. coli messages. To investigate these factors more broadly, we examined the half-lives and steady-state abundance of known and predicted E. coli mRNAs at single-
The National Academy of Sciences.
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5. Time-resolved delayed luminescence image microscopy using an europium ion chelate complex.
Improvements and extended applications of time-resolved delayed luminescence imaging microscopy (TR-DLIM) in cell biology are described. The emission properties of europium ion complexed to a fluorescent chelating group capable of labeling proteins are exploited to provide high contrast images of biotin labeled ligands through detection of the delayed emissi
The Biophysical Society.
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6. Identification of different emitting species in the red fluorescent protein DsRed by means of ensemble and single-molecule spectroscopy
The photophysics and photochemistry taking place in the DsRed protein, a recently cloned red fluorescent protein from a coral of the Discosoma genus, are investigated here by means of ensemble and single-molecule time-resolved detection and spectroscopic measurements. Ensemble time-resolved data reveal that 25% of the immature green chromophores are pre
The National Academy of Sciences.
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7. Solid-phase synthesis of chelate-labelled oligonucleotides: application in triple-color ligase-mediated gene analysis.
Oligonucleotides labelled with detectable groups are essential tools in gene detection. We describe here the synthesis of pyrimidine deoxynucleotide-building blocks, modified at their C-5 position with a protected form of a strongly chelating agent. These reagents can be used to introduce multiple metal ions into oligodeoxynucleotides during standard oligonu
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8. Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins
Fast excitation-driven fluctuations in the fluorescence emission of yellow-shifted green fluorescent protein mutants T203Y and T203F, with S65G/S72A, are discovered in the 10−6–10−3-s time range, by using fluorescence correlation spectroscopy at 10−8 M. This intensity-dependent flickering is conspicuous at high pH, with rate constants independent of
The National Academy of Sciences.
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9. Steady-state and time-resolved fluorescence studies indicate an unusual conformation of 2-aminopurine within ATAT and TATA duplex DNA sequences
2-Aminopurine (2-AP), a fluorescent analog of adenine, has been widely used as a probe for local DNA conformation, since excitation and emission characteristics and fluoresence lifetimes of 2-AP vary in a sequence-dependent manner within DNA. Using steady-state and time-resolved fluorescence techniques, we report that 2-AP appears to be unusually stacked in
Oxford University Press.
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10. Fast synaptic inhibition promotes synchronized gamma oscillations in hippocampal interneuron networks
Networks of GABAergic interneurons are of critical importance for the generation of gamma frequency oscillations in the brain. To examine the underlying synaptic mechanisms, we made paired recordings from “basket cells” (BCs) in different subfields of hippocampal slices, using transgenic mice that express enhanced green fluorescent protein (EGFP) under t
The National Academy of Sciences.
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11. DNA binding induces dissociation of the multimeric form of HIV-1 integrase: A time-resolved fluorescence anisotropy study
Self-assembly of HIV-1 integrase (IN) in solution has been studied previously by time-resolved fluorescence, using tryptophan anisotropy decay. This approach provides information on the size of macromolecules via the determination of rotational correlation times (θ). We have shown that, at submicromolar concentration, IN is characterized by a long rotationa
The National Academy of Sciences.
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12. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion.
The green fluorescent protein (GFP) was used as a noninvasive probe to quantify the rheological properties of cell cytoplasm. GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm. GFP-S65T was brightly fluorescent in solution (lambda ex 492 nm, lambda em 509 nm) with a lifetime of 2.9 ns and a rotat