Footprinting
Mostrando 1-12 de 1494 artigos, teses e dissertações.
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1. Estudos biofísicos de chaperonas de secreção e de interações proteína-ligante / Biophysical studies on secretion chaperones and protein-ligand interactions
So far, the Xanthomonas axonopodis pv. citri (XAC) mechanisms of bacterial virulence is unknown. It is believed that secretion chaperones (CS) are involved in the XAC´s virulence process by first forming complexes with virulence factors, and assisting in their presentation to corresponding secretion systems using ATP as a source of energy. Fluorescence emis
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2012
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2. Estudo da regulação do gene cspD de Caulobacter crescentus. / The study of cspD gene regulation in Caulobacter crescentus.
CspD é uma das quatro proteínas de choque frio de Caulobacter crescentus, sendo maior que as outras CSPs por possuir dois domínios de choque frio, e tem seu papel na célula ainda desconhecido. O objetivo deste trabalho foi identificar e caracterizar os fatores in cis e in trans envolvidos na regulação da expressão do gene cspD em C. crescentus. Neste
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/11/2011
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3. Confiabilidade intra e interexaminador da análise por padrões de impressão de plantigrafias de pessoas diabéticas obtidas com o Harris Mat
INTRODUÇÃO: A hiperpressão plantar é um fator de risco comprovado para a ulceração em portadores de diabetes mellitus. O "Harris and Beath Footprinting Mat" é um dos instrumentos usados nas avaliações para rastreamento do risco de ulceração nos pés desses pacientes. Não há relatos na literatura sobre estudos da confiabilidade da análise das im
Brazilian Journal of Physical Therapy. Publicado em: 2010-06
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4. Use of mass spectrometry, cross-linking and footprinting in the study of protein-protein interactions / Utilização de espectrometria de massas, ligação cruzada (cross-linking) e footprinting no estudo de interações proteina-proteina
A Espectrometria de Massas (MS) é hoje a principal técnica de caracterização de estrutura primária de proteínas devido às vantagens intrínsecas da técnica. As técnicas de cross-linking e footprinting visam desfrutar de tais vantagens para obtenção de informações estruturais de complexos protéicos. Nesse projeto foram realizados estudos de frag
Publicado em: 2009
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5. Províncias diamantíferas de Minas Gerais: uma proposta para a caracterização de populações de diamantes típicas como subsídio à Certificação Kimberley
A partir do pressuposto de que lotes de diamantes provenientes de diferentes regiões possuem assinaturas" mineralógicas típicas, propõe-se uma metodologia que venha a contribuir no sentido de se reconhecer feições específicas em lotes diamantíferos diversos do Estado de Minas Gerais. Esse Estado foi responsável pela posição do Brasil como principa
Publicado em: 2009
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6. Protein antigen-monoclonal antibody contact sites investigated by limited proteolysis of monoclonal antibody-bound antigen: protein "footprinting".
This study describes the use of limited proteolysis of monoclonal antibody (mAb)-bound antigens in the analysis of the two measles virus surface glycoproteins. This approach is dubbed protein "footprinting" in analogy with DNA "footprinting." Protein footprinting was superior to competitive-binding assays and as good as in vitro mAb-selected variant analysis
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7. Footprinting reveals that nogalamycin and actinomycin shuffle between DNA binding sites.
The hypothesis that sequence-selective DNA-binding antibiotics locate their preferred binding sites by a process involving migration from nonspecific sites has been tested by footprinting with DNAase I. Footprinting patterns on the tyrT DNA fragment produced by nogalamycin and actinomycin change with time after mixing the antibiotic with the DNA. Sites of pr
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8. Methidiumpropyl-EDTA.Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA.
DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restriction fragments and Maxam-Gilbert sequencing gel methods of analysis. One method utilizes the enzyme, DNase I, as the DNA cleaving agent while the other employs the synthetic molecule, methidium-propyl-EDTA (MPE). For actinomycin D, chromomycin A3 and distamycin A
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9. Combining chromatin immunoprecipitation and DNA footprinting: a novel method to analyze protein–DNA interactions in vivo
A variety of methods are available to analyze protein–DNA interactions in vivo. Two of the most prominent of these methods are chromatin immunoprecipitation (ChIP) and in vivo footprinting. Both of these procedures have specific limitations. For example, the ChIP assay fails to document where exactly a protein binds in vivo. The precipitation of a specific
Oxford University Press.
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10. High resolution hydroxyl radical footprinting of the binding of mithramycin and related antibiotics to DNA.
The preferred binding sites for mithramycin on three different DNA fragments have been determined by hydroxyl radical footprinting. Sequences which appear as one long protected region using DNAase I as a footprinting probe are resolved into several discrete binding domains. Each drug molecule protects three bases from radical attack, though adjacent regions
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11. DNA sequence preferences for an intercalating porphyrin compound revealed by footprinting.
The DNA sequence preferences of the compound meso-tetra-(4-N-methyl(pyridyl) porphyrin and its nickel complex have been investigated by means of footprinting experiments on several DNA fragments, using DNAase I and micrococcal nuclease as footprinting agents. A complex pattern of both AT and GC-protected sites was found. Ligand-induced long-range conformatio
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12. In vivo DNase I-mediated footprinting analysis along the human bradykinin B1 receptor (BDKRB1) gene promoter: evidence for cell-specific regulation
By applying in vivo dimethyl sulphate and UV light type C-footprinting analysis, we previously showed that specific DNA sequences in the −1349/+42 core promoter region of the inducible human BDKRB1 (bradykinin B1 receptor) gene correlated with its transcriptional activity. In the present study we used the highly sensitive DNase I in vivo footprinting appro
Portland Press Ltd..