Fucosidase
Mostrando 1-12 de 54 artigos, teses e dissertações.
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1. Glycolytic activities in size-fractionated water samples: emphasis on rhamnosidase, arabinosidase and fucosidase activities
Glycolytic activities of eight enzymes in size-fractionated water samples from a eutrophic tropical reservoir are presented in this study, including enzymes assayed for the first time in a freshwater environment. Among these enzymes, rhamnosidase, arabinosidase and fucosidase presented high activity in the free-living fraction, while glucosidase, mannosidase
Brazilian Archives of Biology and Technology. Publicado em: 2011-06
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2. Purification and characterization of cytosolic and cell wall β-galactosidases from Vigna unguiculata stems
Three β-galactosidase isoforms, β-gal I and β-gal II (cytosolic) and β-gal III (cell wall-associated), were isolated from stems of Vigna unguiculata (L.) Walp. cv. Pitiúba seedlings. Purification consisted of aμMonia sulfate fractionation followed by chromatography in DEAE-Sephadex and Lactosyl-Sepharose columns. The two cytosolic isoforms showed the s
Brazilian Journal of Plant Physiology. Publicado em: 2011
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3. Caracterização de isolados do fungo Malassezia pachydermatis através do perfil enzimático
Malassezia spp. são fungos lipofílicos, reconhecidos há mais de cem anos como membros da microbiota cutânea normal humana, bem como agentes de doenças dermatológicas. Desde 1980, são relatados como causadores de infecções sistêmicas oportunistas. Estas leveduras são frequentemente encontradas em uma grande variedade de animais homeotérmicos, e su
Publicado em: 2011
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4. Caracterização dos componentes extracelulares produzidos em cultura de célula de Rubus fruticosus (amora-preta) durante resposta de hipersensibilidade / Characterization of the extracellular compounds released from Rubus fruticosus (blackberry) cell during a hypersensitive response.
The plant-pathogen interactions trigger a series of signals that are not yet completely understood. One of the mechanisms is the hypersensitive response (HR), which is characterized by cell death in the infection site in order to prevent pathogen proliferation. Our previous studies with different elicitors demonstrated the correlation between the formation o
Publicado em: 2009
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5. Analise filogenetica das enzimas hidroliticas de Xiloglucano no reino Viridiplantae e construção de bibliotecas de cDNA de Jatoba (Hymenaea courbaril) / Phylogenetic analysis of Xyloglucan s hydrolytic enzymes in the Viridiplantae kingdom and construction of cDNA libraries from Jatoba (Hymenaea courbaril)
Introdução: Os xiloglucanos são os polímeros de açúcar mais abundantes na hemicelulose da maioria das espécies de plantas terrestres, em especial nas eudicotiledôneas. Possuem papel estrutural na parede celular vegetal, interagindo com os filamentos de celulose, e podem ser utilizados como reserva em sementes de várias espécies de eudicotiledôneas
Publicado em: 2008
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6. Immunocytochemical and biochemical detection of alpha-L-fucosidase in Trypanosoma cruzi
The aim of the present study was to demonstrate the presence of alpha-L-fucosidase in Trypanosoma cruzi. Immunocytochemical and biochemical techniques were used to localize and characterize a membrane-associated, neutral-pH-optimum, alpha-L-fucosidase from the parasite. Light and electron microscopy localized the alpha-L-fucosidase specifically on the surfac
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-05
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7. Detecção de alfa-L-Fucosidade em Trypanosoma Cruzi / Detection of alfa-L-fucosidase from Trypanosoma cruzi
Glicoconjugados são abundantes na superfície de Trypanosoma cruzi e têm sido bastante estudados por diferentes grupos. A degradação dessas moléculas, no entanto, tem sido alvo de pouco interesse. O objetivo deste trabalho foi determinar a atividade de alfa-L-fucosidase em T.cruzi uma vez que trabalhos anteriores haviam concluído que várias hidrolases
Publicado em: 1997
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8. Formation of deglycosylated alpha-L-fucosidase by endo-beta-N-acetylglucosaminidase in Fusarium oxysporum.
Two forms of alpha-L-fucosidase, deglycosylated and glycosylated, were found in the fucose-inducing culture broth of Fusarium oxysporum. Endo-beta-N-acetylglucosaminidase was also found in the same culture broth. The deglycosylated alpha-L-fucosidase was purified from the culture broth to homogeneity on polyacrylamide disc gel electrophoresis and analytical
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9. AtFXG1, an Arabidopsis Gene Encoding α-l-Fucosidase Active against Fucosylated Xyloglucan Oligosaccharides1
An α-l-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an α-l-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single band on SDS-PAGE. Two peptide sequences were ob
American Society of Plant Physiologists.
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10. Fucosidosis: genetic and biochemical analysis of eight cases.
The molecular basis of the deficiency of alpha-L-fucosidase has been investigated in eight patients who had been diagnosed clinically and enzymatically as suffering from the autosomal recessive lysosomal storage disease fucosidosis. None of the patients had a deletion or gross alteration of the alpha-L-fucosidase gene (FUCA1). Single strand conformation poly
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11. Fucosidosis in a native-born Briton.
This investigation reports the biochemical findings in a native-born Briton suffering from the adult form of fucosidosis. Alpha-L-fucosidase, alpha-L-iduronidase, and beta-D-galactosidase were studied in cultured fibroblast and leucocytes from the patient with fucosidosis, her maternal grandfather, and several normal controls. A complete lack of alpha-L-fuco
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12. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening
An efficient β-fucosidase was evolved by DNA shuffling from the Escherichia coli lacZ β-galactosidase. Seven rounds of DNA shuffling and colony screening on chromogenic fucose substrates were performed, using 10,000 colonies per round. Compared with native β-galactosidase, the evolved enzyme purified from cells from the final round showed a 1,000-fold inc
The National Academy of Sciences of the USA.