Final envelopment of the cytoplasmic herpes simplex virus type 1 (HSV-1) nucleocapsid is thought to occur by budding into trans-Golgi network (TGN)-derived membranes. The highly membrane-associated proteins UL20p and glycoprotein K (gK) are required for cytoplasmic envelopment at...
Ver documentoMultiple amino acid changes within herpes simplex virus type 1 (HSV-1) gB and gK cause extensive virus-induced cell fusion and the formation of multinucleated cells (syncytia). Early reports established that syncytial mutations in gK could not cause cell-to-cell fusion in the abs...
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Ver documentoThe herpes simplex virus type 1 (HSV-1) UL20 protein is an important determinant for virion morphogenesis and virus-induced cell fusion. A precise deletion of the UL20 gene in the HSV-1 KOS strain was constructed without affecting the adjacent UL20.5 gene. The resultant KOS/UL20-...
Ver documentoPlasmid NAH7 was transferred from Pseudomonas putida PpG7 to P. putida R20 [R20(NAH7)], an antagonist of Pythium ultimum. The plasmid did not affect growth or survival of R20(NAH7) and was stably maintained under nonselective conditions in broth and soil and on sugar beet seeds. ...
Ver documentoSyncytium formation in cells that express herpes simplex virus glycoprotein B (gB), gD, gH, and gL is blocked by gK (E. Avitabile, G. Lombardi, and G. Campadelli-Fiume, J. Virol. 77:6836-6844, 2003). Here, we report the results of two series of experiments. First, UL20 protein (U...
Ver documentoA crise financeira de 2008 teve impactos significativos no capitalismo global, sendo um de seus reflexos na estrutura da governança global a constituição e evolução do G-20. Neste contexto, o objetivo do artigo é analisar tais mudanças e, em especial, as posições de quatro dos pr...
Ver documentoA partir de 2000, na cúpula de Okinawa, o sistema G7/8 começou um movimento de outreach, ou seja, tanto de "alcançar" aqueles que se encontravam fora quanto de "expandir" o sistema G7/8. Neste sentido, pela primeira vez países não-participantes do G8 foram envolvidos tanto em enc...
Ver documentoThis article analyzes Brazil's performance during the agricultural negotiations in the Doha Round, considering the implications of domestic backing for the country's credibility vis-à-vis the G-20 and the coalition's contribution to increasing Brazil's bargaining power in relatio...
Ver documentoABSTRACT VC (20%), TC (20%) and N:P:K fertilizer (farmer's practice) were used to determine the growth and yield attributes of bush bean (Phaseolus vulgaris), winged bean (Psophocarpus tetragonolobus) and yard long bean (Vigna unguiculata). Plants grown with VC (20%) produced the...
Ver documentoRESUMO O presente trabalho visa investigar os efeitos do extrato de semente de uva Proanthocyanidin (GSPE) sobre o desempenho da produção, o metabolismo e o status antioxidante de gado leiteiro Holstein em lactação precoce. Quarenta e oito vacas leiteiras multíparas Holstein fora...
Ver documentoThe 2008 financial crisis stands out for being a crisis that occurred not in developing countries, but in the core capitalist countries, thus assuming greater proportions and with broader ramifications. In this context, the G20 gained new impetus, and, as a result, several studie...
Ver documentoGlycoprotein K (gK) of pseudorabies virus (PrV) has recently been identified as a virion component which is dispensable for viral entry but required for direct cell-to-cell spread. Electron microscopic data suggested a possible function of gK in virus egress by preventing immedia...
Ver documentoThe role of glycoprotein E (gE) and gI of Marek's disease virus serotype 1 (MDV-1) for growth in cultured cells was investigated. MDV-1 mutants lacking either gE (20ΔgE), gI (20ΔgI), or both gE and gI (20ΔgEI) were constructed by recE/T-mediated mutagenesis of a recently establis...
Ver documentoAim of the present study was the comparative evaluation of the effect of ground oregano, anise and olive leaves as feed additives on performance and some egg quality characteristics of laying Japanese quails. A total of 189 Coturnix japonica quails (126 females and 63 males), 149...
Ver documentoInfiltration of enamel-like initial caries lesions with low viscosity resins (infiltrants) seems to be a promising approach in dentistry and it have showed advantages regarding to remineralization or invasive treatments. In this way, material must penetrate and seal lesions intak...
Ver documentoO objetivo desta dissertação consiste em analisar a atuação da coalizão G-20, no âmbito da Rodada Doha, da OMC, tendo como foco principal o entendimento das posições brasileiras na coalizão. Procuramos compreender a receptividade da política externa brasileira às pressões domésti...
Ver documentoEste trabalho avaliou a resistência à tração e alongamento de amostras de diferentes diâmetros (1,5, 2,0 e 5,0 mm) fundidas em titânio comercialmente puro (Tritan - Dentaurum), submetidas a soldagem a laser em diferentes distâncias de soldagem (0,0 mm e 0,6 mm). Foram confecciona...
Ver documentoOBJETIVO: Avaliar o efeito do glutamato monossódico (GMS) nos fetos de ratas prenhes por meio da comparação do peso, comprimento nasal-anal (CNA) e índice de Lee (IL) ao nascimento e com 21 dias de vida. MÉTODOS: Foram utilizadas ratas prenhes da linhagem Wistar distribuídas em t...
Ver documentoO objetivo deste trabalho foi avaliar a penetração de materiais resinosos experimentais de baixa viscosidade em lesões artificiais sub-superficiais de cárie em esmalte (LASCE), por meio de Microscopia Confocal de Varredura a Laser (MCVL). Cinquenta e cinco terceiros molares human...
Ver documentoRESUMO Objetivo: Verificar se o Hylan G-F20 melhora os resultados da lavagem e injeção de triancinolona na osteoartrite do quadril (OAQ). Métodos: 82 pacientes com HOA Kellgren e Lawrence graus II e III foram randomizados: lavagem e triamcinolona (G0); lavagem, triancinolona ...
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