Gene Virb10
Mostrando 1-12 de 32 artigos, teses e dissertações.
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1. Desenvolvimento e avaliação de uma cepa knockout de Brucella abortus obtida pela deleção do gene virB10
Brucella spp. são bactérias gram-negativas, intracelulares facultativas que são patogênicas para muitas espécies de mamíferos causando a brucelose, uma zoonose difundida mundialmente. Por isso a busca de alternativas de controle mais eficientes se faz necessário como o desenvolvimento de novas cepas que possam ser testadas como potenciais imunógenos.
Pesquisa Veterinária Brasileira. Publicado em: 2009-11
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2. Development and evaluation of residual virulence of a mutant strain of Brucella Abortus / Desenvolvimento e avaliação da virulência residual de uma cepa mutante de Brucella abortus
Brucella spp. is an intracellular facultative gram-negative bacteria which is pathogenic for many species of mammals, causing brucelosis, a worldwide spread zoonosis. Therefore the search for more efficient alternatives of control, as the development of new strains that can be tested as potential immunogens, is necessary. In this study, we knockouted virB10
Publicado em: 2009
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3. Transcrição de genes de proteínas de membrana de isolados brasileiros de Anaplasma marginale
Este trabalho demonstra o padrão de transcrição de genes de proteínas de membrana em três isolados brasileiros de A. marginale (Rio Grande do Norte, Pernambuco-Zona da Mata e Pernambuco-Sertão). O RNA foi purificado a partir de sangue de bovinos infectados experimentalmente com os três isolados de A. marginale. Após transcrição reversa, os genes om
Revista Brasileira de Parasitologia Veterinária. Publicado em: 2007-09
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4. Transcription of genes of membranes proteins in brazilians isolates of Anaplasma marginale / Transcrição de genes para proteinas de membrana de isolados brasileiros de Anaplasma marginale
Este trabalho demonstra o padrão de transcrição de genes de proteinas de membrana em tres isolados brasileiros de A. marginale (Rio Grande do Norte, Pernambuco-Zona da Mata e Pernambuco-Sertão). RNA foi purificado a partir de sangue de bovinos infectados experimentalmente com os tres isolados de A. marginale. Após transcrição reversa, os genes omp1, 2
Publicado em: 2006
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5. Complementation analysis of Agrobacterium tumefaciens Ti plasmid virB genes by use of a vir promoter expression vector: virB9, virB10, and virB11 are essential virulence genes.
The virB gene products of the Agrobacterium tumefaciens tumor-inducing (Ti) plasmid have been proposed to mediate T-DNA transport through the bacterial cell wall into plant cells. Previous genetic analysis of the approximately 9.5-kilobase-pair virB operon has been limited to transposon insertion mutagenesis. Due to the polarity of the transposon insertions,
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6. Identification of a virB10 protein aggregate in the inner membrane of Agrobacterium tumefaciens.
Products of the virB operon are proposed components of a membrane-associated T-DNA transport apparatus in Agrobacterium tumefaciens. Here we identified the virB10 gene product and raised specific antiserum to the protein. While the virB10 reading frame contains two potential ATG translation start sites located 32 codons apart, we found that only the downstre
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7. Activity of the Agrobacterium T-DNA transfer machinery is affected by virB gene products.
The oriT (origin of transfer) sequence and mob (mobilization) genes of plasmid RSF1010 can functionally replace transfer DNA (T-DNA) borders to generate an RSF1010 intermediate transferable to plants through activities of the tumor-inducing (Ti)-plasmid virulence (vir) genes of Agrobacterium tumefaciens. Because the Ti plasmid virB gene products are hypothes
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8. Interactions of VirB9, -10, and -11 with the membrane fraction of Agrobacterium tumefaciens: solubility studies provide evidence for tight associations.
The eleven predicted gene products of the Agrobacterium tumefaciens virB operon are believed to form a transmembrane pore complex through which T-DNA export occurs. The VirB10 protein is required for virulence and is a component of an aggregate associated with the membrane fraction of A. tumefaciens. Removal of the putative membrane-spanning domain (amino ac
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9. Characterization and Transcriptional Analysis of Gene Clusters for a Type IV Secretion Machinery in Human Granulocytic and Monocytic Ehrlichiosis Agents
Anaplasma (Ehrlichia) phagocytophila and Ehrlichia chaffeensis, the etiologic agents of granulocytic and monocytic ehrlichioses, respectively, are obligatory intracellular bacteria that cause febrile systemic illness in humans. We identified and characterized clusters of genes for a type IV secretion machinery in these two bacteria, and analyzed their gene e
American Society for Microbiology.
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10. Intracellular Induction of the Bartonella henselae virB Operon by Human Endothelial Cells
One of the more recently identified bacterial exportation systems is the type IV secretion mechanism, which is characterized by a multiprotein complex that spans the inner and outer bacterial membranes and contains a pilin component. The most thoroughly studied type IV secretion system is encoded by the virB operon of Agrobacterium tumefaciens. In Bartonella
American Society for Microbiology.
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11. A Homologue of an Operon Required for DNA Transfer in Agrobacterium Is Required in Brucella abortus for Virulence and Intracellular Multiplication
As part of a Brucella abortus 2308 genome project carried out in our laboratory, we identified, cloned, and sequenced a genomic DNA fragment containing a locus (virB) highly homologous to bacterial type IV secretion systems. The B. abortus virB locus is a collinear arrangement of 13 open reading frames (ORFs). Between virB1 and virB2 and downstream of ORF12,
American Society for Microbiology.
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12. The Agrobacterium tumefaciens virB7 gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface.
The Agrobacterium tumefaciens virB7 gene product contains a typical signal sequence ending with a consensus signal peptidase II cleavage site characteristic of bacterial lipoproteins. VirB7 was shown to be processed as a lipoprotein by (i) in vivo labeling of native VirB7 and a VirB7::PhoA fusion with [3H]palmitic acid and (ii) inhibition of VirB7 processing