Genes Amplification
Mostrando 1-12 de 1524 artigos, teses e dissertações.
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1. Emerging carbapenem-resistant Aeromonas spp. infections in Cali, Colombia
ABSTRACT Introduction: Aeromonas species are renowned enteric pathogens with virulence determinants linked to human diseases, such as gastroenteritis, skin, soft-tissue and muscle infections, and septicemia. A recent concern of resistance in this organism has emerged, especially the presence carbapenemases. Herein we describe a case series of emerging carba
Braz J Infect Dis. Publicado em: 25/11/2019
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2. Antibiotic resistance in surface waters from a coastal lagoon of Southern Brazil under the impact of anthropogenic activities
Resumo Resíduos oriundos de atividades humanas podem atingir corpos d'água e contribuir significativamente para a presença de populações bacterianas resistentes a antibióticos. O objetivo deste estudo foi avaliar populações de bactérias cultiváveis resistentes a antibióticos em uma lagoa costeira impactada por atividades como agricultura e urbaniz
Rev. Ambient. Água. Publicado em: 10/10/2019
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3. Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil
RESUMO: Toxina A de Clostridium chauvoei (CctA), neuraminidase (NanA) e flagelina (FliC) são proteínas que contribuem para a patogenicidade de Clostridium chauvoei, o agente causador do carbúnculo sintomático em bovinos. O objetivo deste estudo foi analisar a variabilidade genética dos genes cctA, nanA, e fliC em C. chauvoei isolados em diferentes per�
Cienc. Rural. Publicado em: 02/05/2019
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4. Molecular characterization of a phytoplasma associated with a commercial variety of Momordica charantia
ABSTRACT: Momordica charantia (bitter melon) presents two distinct types or varieties, known as wild type and commercial type. Plants of the wild type are hosts of a phytoplasma of the group 16SrIII-J, which is associated with a disease known as witches’ broom. However, this disease has not yet been reported in commercial bitter melon. Thus, symptomatic pl
Sci. agric. (Piracicaba, Braz.). Publicado em: 2019-05
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5. Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
BACKGROUND AND OBJECTIVE Brazil is responsible for a large number of Plasmodium vivax cases in America. Given the emergence of P. vivax parasites resistant to chloroquine and the effectiveness of antifolates in vivax malaria treatment together with a correlation between mutations in P. vivax dhfr and dhps genes and SP treatment failure, the point mutations
Mem. Inst. Oswaldo Cruz. Publicado em: 04/02/2019
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6. Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest
Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The
Braz. J. Microbiol.. Publicado em: 2018-09
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7. Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I
Braz. J. Microbiol.. Publicado em: 2018-06
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8. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum
ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great diffic
An. Acad. Bras. Ciênc.. Publicado em: 2018-03
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9. Paracoccidioidomycosis due to Paracoccidioides brasiliensis S1 plus HIV co-infection
BACKGROUND Paracoccidioidomycosis (PCM) is one of the most important systemic mycoses in Latin America and the leading fungal cause of mortality in non-immunosuppressed individuals in Brazil. However, HIV/PCM co-infection can increase the clinical severity in these co-infected patients. This co-infection is rarely reported in the literature mainly because o
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-03
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10. Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras
BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. OB
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-01
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11. Captive wild birds as reservoirs of enteropathogenic E. coli (EPEC) and Shiga-toxin producing E. coli (STEC)
ABSTRACT Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 diffe
Braz. J. Microbiol.. Publicado em: 2017-12
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12. Simple protocol for population (Sanger) sequencing for Zika virus genomic regions
BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE The aim of this study was to develop a simple and robu
Mem. Inst. Oswaldo Cruz. Publicado em: 27/11/2017