Genetic Modified Mice
Mostrando 1-12 de 50 artigos, teses e dissertações.
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1. Chromatin supraorganization and extensibility, and nuclear composition in mouse cells / Supraorganização e extensibilidade da cromatina, e composição nuclear em celulas de camundongo
Aging may be defined as the changes that take place in an organism with time. This process, in biology, is called senescence. Cellular senescence is observed in isolated cells, and has been studied typically in cultured cells, but its occurrence in vivo has been shown only in some mammalian tissues. Chromatin changes that take place with cellular senescence
Publicado em: 2008
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2. Lack of correlation between rabies virus replication in the brain and antibody isotype profile in genetically modified mice
The relationship among the phenotypes resistance to infection, virus replication in the brain and isotype production was investigated in genetically modified High (H) or Low (L) antibody responder mouse lines. Although they express the same innate susceptibility to rabies infection, these lines differ as to different viral replication rates in the central ne
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2006
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3. Efeito da terapia com Beta-bloqueadores em camundongos com deleção dos receptores alpha 2A/alpha 2C adrenérgicos / Effects of b- adrenergic antagonist therapy in mice lacking a 2A/a 2C adrenergic receptors
We have recently reported that disruption for both a2A ?and a2C adrenergic receptor subtypes in mice (KO) leads to sympathetic hyperactivity with evidence of heart failure (HF) by the age of 7 months. These mice provide a model system for evaluating the efficiency among different ?- adrenergic antagonists (BB) for HF therapy. In the present study, we evaluat
Publicado em: 2006
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4. A Recombinant Measles Virus Expressing Hepatitis B Virus Surface Antigen Induces Humoral Immune Responses in Genetically Modified Mice
It has been shown previously that measles virus (MV) can be successfully used to express foreign proteins (M. Singh and M. A. Billeter, J. Gen. Virol. 80:101–106, 1998). To develop an inexpensive MV-based vaccine, we generated recombinant MVs that produce structural proteins of hepatitis B virus (HBV). A recombinant virus that expressed the HBV small surfa
American Society for Microbiology.
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5. Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.
We have generated transgenic mouse lines carrying and expressing wild-type and 3'-modified human tumour necrosis factor (hTNF-alpha, cachectin) transgenes. We show that correct, endotoxin-responsive and macrophage-specific hTNF gene expression can be established in transgenic mice and we present evidence that the 3'-region of the hTNF gene may be involved in
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6. Strain-dependent Differences in LTP and Hippocampus-dependent Memory in Inbred Mice
Many studies have used “reverse” genetics to produce “knock-out” and transgenic mice to explore the roles of various molecules in long-term potentiation (LTP) and spatial memory. The existence of a variety of inbred strains of mice provides an additional way of exploring the genetic bases of learning and memory. We examined behavioral memory and LTP
Cold Spring Harbor Laboratory Press.
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7. A genetic model for absent chylomicron formation: mice producing apolipoprotein B in the liver, but not in the intestine.
The formation of chylomicrons by the intestine is important for the absorption of dietary fats and fat-soluble vitamins (e.g., retinol, alpha-tocopherol). Apo B plays an essential structural role in the formation of chylomicrons in the intestine as well as the VLDL in the liver. We have developed genetically modified mice that express apo B in the liver but
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8. High efficiency genetic modification of hair follicles and growing hair shafts
A technique for genetic modification of hair follicles was developed which results in efficient alteration of the hair shaft phenotype. High-level in vivo transgene expression was maintained in hair follicles such that growing hair shafts were phenotypically altered. Mouse anagen skin fragments, maintained in histoculture, were genetically modified at high e
The National Academy of Sciences.
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9. A murine model of myeloma that allows genetic manipulation of the host microenvironment
Multiple myeloma, and the associated osteolytic bone disease, is highly dependent upon cellular interactions within the bone marrow microenvironment. A major limitation of existing myeloma models is the requirement for a specific host strain of mouse, preventing molecular examination of the bone marrow microenvironment. The aim of the current study was to de
The Company of Biologists Limited.
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10. An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues
A common method for generating mice with subtle genetic manipulations uses homologous recombination (HR) in embryonic stem (ES) cells to replace a wild-type gene with a slightly modified one. Generally, a drug resistance gene is inserted with the modified gene to select correctly targeted clones. Often, however, the presence of this drug resistance gene inte
Oxford University Press.
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11. Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis
We evaluated the adoptive transfer of DCs on Leishmania (L.) mexicana-infected neonatal BALB/c mice. DCs were isolated and purified from the spleens of the following donor groups: a) Adult BALB/c mice infected during adulthood with L. (L) mexicana; b) Adult BALB/c mice infected during neonatal life; c) Healthy neonatal BALB/c mice; d) Healthy adult BALB/c mi
BioMed Central.
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12. The Use of the R6 Transgenic Mouse Models of Huntington's Disease in Attempts to Develop Novel Therapeutic Strategies
Summary: Huntington's disease (HD) is a genetic neurodegenerative disorder. Since identification of the disease-causing gene in 1993, a number of genetically modified animal models of HD have been generated. The first transgenic mouse models, R6/1 and R6/2 lines, were established 8 years ago. The R6/2 mice have been the best characterized and the most widely
The American Society for Experimental NeuroTherapeutics.