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Comparative Study of Pressure- and Nutrient-Induced Germination of Bacillus subtilis Spores

Germination experiments with specific germination mutants of Bacillus subtilis, including a newly isolated mutant affected in pressure-induced germination, suggest that a pressure of 100 MPa triggers the germination cascades that are induced by the nutrient germinant alanine (Ala...

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Ultrahigh-Temperature Activation of a Low-Temperature Bacillus subtilis Spore Germination System 12

Exposure of Bacillus subtilis A spores to 115 to 125 C for several seconds activated a low-temperature germination system that remained dormant after a heat treatment that activated the normal l-alanine- and glucose-stimulated germination systems. The low-temperature germination ...

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A Role for Brassinosteroids in Germination in Arabidopsis1

This paper presents evidence that plant brassinosteroid (BR) hormones play a role in promoting germination. It has long been recognized that seed dormancy and germination are regulated by the plant hormones abscisic acid (ABA) and gibberellin (GA). These two hormones act anta...

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Microcyst Germination in Myxococcus xanthus1

Germination of glycerol-prepared microcysts of Myxococcus xanthus was studied. The sequence of morphological events during germination resembled that of germinating fruiting body-microcysts. The turbidity drop of a culture of germinating microcysts could be described by McCormick...

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Biochemistry of L-proline-triggered germination of Bacillus megaterium spores.

The mechanism by which L-proline triggers germination in Bacillus megaterium QM B1551 spores was investigated. First, brief exposure of spores to L-proline, followed by dilution, was sufficient to trigger germination. Once germination was triggered, the spores continued initiatio...

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Regulation and Self-Inhibition of Microsporum gypseum Macroconidia Germination

Germination of Microsporum gypseum macroconidia was accompanied by the release of alkaline protease, calcium ions, and inorganic phosphate into the germination fluid. The rate of germination was greatest during the first 2 hr, decreasing thereafter. This decrease in rate was acco...

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Factors Affecting the Germination of Akinetes of Nodularia spumigena (Cyanobacteriaceae)

Nutritional and physical factors which influence the germination of akinetes of Nodularia spumigena (Cyanobacteriaceae) were examined. Low concentrations of phosphorus (45 μM, inhibi...

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The Effect of α-Amanitin on the Arabidopsis Seed Proteome Highlights the Distinct Roles of Stored and Neosynthesized mRNAs during Germination1

To investigate the role of stored and neosynthesized mRNAs in seed germination, we examined the effect of α-amanitin, a transcriptional inhibitor targeting RNA polymerase II, on the germination of nondormant Arabidopsis seeds. We used transparent testa mutants, of which seed coat...

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Characterization of germination and activation of Bdellovibrio bdellocysts.

A simple method of assaying germination of bdellocysts in liquid medium has been devised. Bdellocysts can be induced to germinate by any of eight L-amino acids or the monovalent cations K+ and NH4+. L-Glutamine was the best individual inducer of germination, although the resultin...

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Comparative Study of Pressure-Induced Germination of Bacillus subtilis Spores at Low and High Pressures

We have studied pressure-induced germination of Bacillus subtilis spores at moderate (100 MPa) and high (500 to 600 MPa) pressures. Although we found comparable germination efficiencies under both conditions by using heat sensitivity as a criterion for germination, the sensitivit...

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CALCIUM DIPICOLINIC ACID-INDUCED GERMINATION OF BACILLUS CEREUS SPORES

Keynan, A. (University of Wisconsin, Madison) and H. O. Halvorson. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J. Bacteriol. 83:100–105. 1962.—The germination of spores of Bacillus cereus strain T can be initiated by calcium dipicolinic acid. The kinet...

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Southern highland papaya (Vasconcellea quercifolia A. St.-Hil.): Do fruit ripening and harvesting time affect seed germination?

ABSTRACT. In this work, we report the effects of the harvesting time, the stages in fruit ripening and the influence of potassium nitrate in V. quercifolia seed germination. In addition, information about the storage period and light requirements is provided. Fruits were harveste...

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EFFECT OF SPORULATION MEDIUM ON HEAT RESISTANCE, CHEMICAL COMPOSITION, AND GERMINATION OF BACILLUS MEGATERIUM SPORES1

Levinson, Hillel S. (U.S. Army Natick Laboratories, Natick, Mass.), and Mildred T. Hyatt. Effect of sporulation medium on heat resistance, chemical composition, and germination of Bacillus megaterium spores. J. Bacteriol. 87:876–886. 1964.—Bacillus megaterium spores, grown on var...

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Physiological and biochemical changes during coffee seed germination (Coffea arabica L.) cv. Rubi. / AlteraÃÃes fisiolÃgicas e bioquÃmicas durante a germinaÃÃo de sementes de cafà (coffea arabica L.) cv. Rubi

Coffee propagation is performed through seedlings from seeds. However, germination in coffee seed is slow and uneven. Therefore, this work had the objective of studding the physiological aspects of seed germination in Coffea arabica cv. Rubi. There were evaluated effect of light,...

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Phytochrome Control of Germination of Rumex crispus L. Seeds Induced by Temperature Shifts

High germination of curly dock (Rumex crispus L.) seeds is evident after suitable imbibition and temperature shift treatment, but germination at constant temperatures fails without an input of far red-absorbing form of phytochrome. Preliminary imbibitions at high temperatures (30...

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Identification of a Germination System Involved in the Heat Injury of Bacillus Subtilis Spores 1

Bacillus subtilis A spores were injured by exposure to heat treatments of 110 to 132 C. Injury was demonstrated by the inability to form colonies on fortified nutrient agar (FNA) unless the medium was supplemented with CaCl2 and Na2 dipicolinate (CNA). A preliminary heat treatmen...

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Photocontrol of the Germination of Onoclea Spores: I. Action Spectrum

Light stimulates the germination of spores of the fern Onoclea sensibilis L. At high dosages, broad band red, far red, and blue light promote maximal germination. Maximal sensitivity to these spectral regions is attained from 6 to 48 hours of dark presoaking, and all induced rapi...

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Mannose Inhibits Arabidopsis Germination via a Hexokinase-Mediated Step1

Low concentrations of the glucose (Glc) analog mannose (Man) inhibit germination of Arabidopsis seeds. Man is phosphorylated by hexokinase (HXK), but the absence of germination was not due to ATP or phosphate depletion. The addition of metabolizable sugars reversed the Man-me...

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Effects of Cellulolytic Ruminal Bacteria and of Cell Extracts on Germination of Euonymus americanus L. Seeds

In past attempts, the experimental germination of the seeds of Euonymus americanus L. in vitro has had little success. However, treatment of seeds with ruminal fluid containing viable microflora has been successful in stimulating germination. In the presence of the cellulolytic r...

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Biochemical Studies of Bacterial Sporulation and Germination XVII. Sulfhydryl and Disulfide Levels in Dormancy and Germination

A fourfold increase in sulfhydryl content upon germination of Bacillus megaterium spores was observed by the standard fluorescein mercuric acetate assay as reported by others. However, assay of ruptured dormant spores or the use of N-ethylmaleimide and a denaturing agent on intac...

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Inhibition of Cottonseed Germination with Abscisic Acid and Its Reversal

Germination of cottonseed (Gossypium hirsutum L.) was inhibited by abscisic acid. Inhibition was greater when seeds were soaked in abscisic acid for 5 hours and dried prior to germination than when abscisic acid was applied in the germination medium. (2-Chloroethyl)phosphonic aci...

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