Gfp Green Fluorescent Protein
Mostrando 1-12 de 778 artigos, teses e dissertações.
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1. The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was indu
Int. braz j urol.. Publicado em: 2018-06
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2. Bone marrow cell migration to the heart in a chimeric mouse model of acute chagasic disease
BACKGROUND Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-08
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3. Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics
Abstract Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue in
Braz. J. Microbiol.. Publicado em: 2017-03
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4. Rabbit olfactory stem cells. Isolation protocol and characterization
PURPOSE: To describe a new technique for isolation of a mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits. METHODS: Olfactory stem cells (OSCs) were retrieved from under the cribriform plate of the Ethmoid bone. Several assays were accomplished to characterize the cell population and attest its viability in vitro. The cells were
Acta Cir. Bras.. Publicado em: 2016-01
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5. Use of green fluorescent protein to monitor
Lactobacillus plantarum in the gastrointestinal tract of goatsThe experiment aimed to specifically monitor the passage of lactobacilli
in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter fromL. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated intoBraz. J. Microbiol.. Publicado em: 2015-09
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6. A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones w
Braz J Med Biol Res. Publicado em: 21/07/2015
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7. Study of the antimalarial properties of hydroxyethylamine derivatives using green fluorescent protein transformed Plasmodium berghei
A rapid decrease in parasitaemia remains the major goal for new antimalarial drugs and thus, in vivo models must provide precise results concerning parasitaemia modulation. Hydroxyethylamine comprise an important group of alkanolamine compounds that exhibit pharmacological properties as proteases inhibitors that has already been proposed as a new class of an
Mem. Inst. Oswaldo Cruz. Publicado em: 26/05/2015
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8. Construction of three new Gateway® expression plasmids for Trypanosoma cruzi
We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an e
Mem. Inst. Oswaldo Cruz. Publicado em: 21/11/2014
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9. Bone marrow mesenchymal stem cells overexpressing human basic fibroblast growth factor increase vasculogenesis in ischemic rats
Administration or expression of growth factors, as well as implantation of autologous bone marrow cells, promote in vivo angiogenesis. This study investigated the angiogenic potential of combining both approaches through the allogenic transplantation of bone marrow-derived mesenchymal stem cells (MSCs) expressing human basic fibroblast growth factor (hbFGF).
Braz J Med Biol Res. Publicado em: 08/08/2014
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10. Effects of Human Umbilical Cord Mesenchymal Stem Cells on Renal Ischaemia-reperfusion Injury in Rats
Objective This study aims to observe the function of umbilical cord-mesenchymal stem cells (UC-MSCs) labelled with enhanced green fluorescent protein (eGFP) in the repair of renal ischaemia-reperfusion (I/R) injury, to determine the effects on inflammatory cascade in an established rat model and to explore possible pathogenesis. Materials and Methods Si
Int. braz j urol.. Publicado em: 2014-08
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11. Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique
O replicon de RNA derivado do genoma de Flavivirus é uma ferramenta valiosa para o estudo de replicação viral independente da montagem e da maturação do virion, além de possuir um grande potencial para expressão de genes heterólogos. Neste estudo nós descrevemos a construção de replicons subgenômicos do vírus da febre amarela utilizando a técni
An. Acad. Bras. Ciênc.. Publicado em: 01/03/2013
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12. Otimização de um plasmídeo vacinal por introdução de elementos de direcionamento nuclear a ser veiculado por Lactococcus lactis
Lactococcus lactis, a bactéria láctica modelo, é considerada segura e vem sendo há muito utilizada para produção e entrega de antígenos e citocinas às superfícies de mucosas. Mais recentemente, os estudos envolvendo este microorganismo têm também focado em sua utiilização como veículo para entrega de vacinas de DNA, plataforma vacinal inovadora
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012