Glass Coverslips
Mostrando 1-12 de 29 artigos, teses e dissertações.
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1. Modulação do transporte de prótons em osteoclastos: efeitos da acidose e do fluxo de fluido extracelular. / Modulation of proton transport in osteoclasts. Effects of acidosis and extracellular fluid flow.
Metabolic acidosis can cause a loss of bone mineral and the mechanic stimulation can cause adaptative bone remodeling. The bone resorption characteristic of these bone changes aforementioned depends on the extracellular acidification by osteoclastmediated proton secretion. The H+ secretion by vacuolar H+-ATPase together with Clsecretion through a Cl-/H+ exch
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/10/2011
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2. Efeito da laserterapia de baixa potência Gálio-Alumínio-Índio- Fósforo na inflamação granulomatosa experimental em camundongos / Effects of low level laser therapy Gallium-Aluminum-Indian- Phosphor on the experimental granulomatous inflammation process in albinic mice
Low level laser therapy (LLLT) is a therapeutic strategy in which its effects are still in a continuous process of evolution. The aim of the present study was to identify the effects of LLLT Ga-Al-In-P 660nm on the dynamics of a granulomatous inflammatory process in male swiss albinic mice. The migration pattern of inflammatory cells in glass coverslips inse
Publicado em: 2008
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3. Experimental infection of Leishmania (L.) chagasi in a cell line derived from Lutzomyia longipalpis (Diptera:Psychodidae)
The present work describes the in vitro infection of a cell line Lulo, derived from Lutzomyia longipalpis embryonic tissue, by Leishmania chagasi promastigotes. This infection process is compared with a parallel one developed using the J774 cell line. The L. chagasi MH/CO/84/CI-044B strain was used for experimental infection in two cell lines. The cells were
Memórias do Instituto Oswaldo Cruz. Publicado em: 2005-10
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4. Ação do perfluoroctano em cultura de celulas vero
Culture of Vero cells was used as a model to evaluate the action of one of the .liquid perfluorocarbons (PFCL) most commonly used in ophthalmology in vitreo retinal surgeries, The Perfluoroctane (PFOC) has among other characteristics a high specific weight, which consequently facilitates the replacement of the retina. Analyses were made of the damage incurre
Publicado em: 1999
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5. Treated glass coverslips for in situ culture of amniotic cells: better cell adhesion and growth than on plastic.
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6. Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution.
Scanning force microscopy allows imaging of biological molecules in their native state in buffer solution. To this end samples have to be fixed to a flat solid support so that they cannot be displaced by the scanning tip. Here we describe a method to achieve the covalent binding of biological samples to glass surfaces. Coverslips were chemically modified wit
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7. Membrane skeleton in fresh unfixed erythrocytes as revealed by a rapid-freezing and deep-etching method.
A rapid-freezing and deep-etching method for examining en face the cytoplasmic aspects of unfixed erythrocyte membranes is described, which provides improved resolution. Normal human erythrocytes were centrifuged, washed in a phosphate buffer solution and pelleted. Glass coverslips were coated with 3-aminopropyl triethoxy silane and glutaraldehyde to make er
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8. Attachment of Treponema pallidum to fibronectin, laminin, collagen IV, and collagen I, and blockage of attachment by immune rabbit IgG.
As shown by scanning electron and phase contrast microscopy, Treponema pallidum attached in vitro to basement membranes purified from kidney cortex tissues or from retinal vessels. This organism also attached to the extracellular matrix remaining after cultured cells had been solubilised with Triton X. Fibronectin, laminin, collagen, IV, collagen I, and hyal
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9. Periodic structures in lipid monolayer phase transitions
Periodic patterns are observed when supported lipid monolayers doped with low concentrations of fluorescent lipid probes are observed with epi-fluorescence microscopy. Monolayers of dipalmitoyl phosphatidylcholine were examined on air-water interfaces and also on alkylated glass coverslips. The patterns are formed by periodic arrays of solid-phase lipid doma
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10. Topological and modulated distribution of surface markers on endothelial cells.
The mobility and distribution of angiotensin-converting enzyme (peptidyl-dipeptide hydrolase, EC 3.4.15.1) and a specific endothelial cell surface protein was assessed by fluorescein-conjugated monoclonal antibodies on bovine and murine endothelial cells grown on their extracellular matrix. The combination of data obtained from fluorescence recovery after ph
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11. Scanning force microscopy reveals ellipsoid shape of chicken erythrocyte nucleosomes.
Scanning force microscopy was used to investigate the conformation of hypotonic spread chicken erythrocyte nucleosomes. Nucleosomal chains were prepared in low-salt conditions and fixed before centrifugation onto glass coverslips and air drying. The images of single nucleosomes were isolated by image processing, and the height and geometry of the resulting t
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12. Antigen presentation by supported planar membranes containing affinity-purified I-Ad.
I-Ad, purified from A20-1.11 cells by affinity chromatography, was incorporated into supported planar membranes by incubation of I-Ad-containing phospholipid vesicles with clean glass coverslips. Such planar membranes present a peptide digest of ovalbumin to the ovalbumin-specific, I-Ad-restricted T-cell hybridoma 3DO-54.8, resulting in the antigen-specific