Gliadins
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Perfil protéico e uso de marcadores moleculares relacionados à qualidade de panificação em trigo / Protein profile and use of molecular markers related to baking quality of wheat
O trigo é um dos principais cereais em todo o mundo, sendo processado para uma gama de produtos. Surgiu nas últimas décadas a preocupação dos pesquisadores com a obtenção de cultivares com boa qualidade de panificação, característica que refere-se primordialmente a constituição protéica do endosperma, composta principalmente pelas proteínas for
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/07/2010
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2. Efeito da adição farinha de soja desengordurada como fonte de lipoxigenase ativade soja nas caracteristicas reologicas ena distribuição dos lipidios na massa defarinha de trigo
The objective of this work was to study the effect of lipoxygenase from defatted soy bean flour on the rheological properties of doughs and on the composition of lipids in doughs and lipids bound to gluten and its fractions. The rheological tests indicated that the addiction of lipoxygenase improves the -dough strength. Upon addiction of lipoxygenase, increa
Publicado em: 1990
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3. Two Closely Related Wheat Storage Proteins Follow a Markedly Different Subcellular Route in Xenopus laevis Oocytes.
[alpha]-Gliadins and [gamma]-gliadins are two closely related wheat storage proteins that evolved from a common ancestral gene. However, synthesis of [alpha]-gliadins and [gamma]-gliadins in Xenopus laevis oocytes revealed striking differences in their subcellular routing. The major portion of [alpha]-gliadin accumulated inside the oocyte, whereas most of th
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4. Thioredoxin-linked mitigation of allergic responses to wheat
Thioredoxin, a ubiquitous 12-kDa regulatory disulfide protein, was found to reduce disulfide bonds of allergens (convert S—S to 2 SH) and thereby mitigate the allergenicity of commercial wheat preparations. Allergenic strength was determined by skin tests with a canine model for food allergy. Statistically significant mitigation was observed with 15 o
The National Academy of Sciences of the USA.
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5. Gene-dosage compensation of endosperm proteins in hexaploid wheat Triticum aestivum
Several aneuploid lines and one intervarietal substitution line of the hexaploid wheat Triticum aestivum (2n = 6x = 42; genomes AABBDD) cv. Chinese Spring were used to study the effects of different doses of chromosomes 1B, 1D, or 1A on the amount of the high molecular weight (“HMW”) glutenins and gliadins of endosperm. These homeologous chromosomes carr
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6. Nucleic acid (cDNA) and amino acid sequences of alpha-type gliadins from wheat (Triticum aestivum).
The complete amino acid sequence for an alpha-type gliadin protein of wheat (Triticum aestivum Linnaeus) endosperm has been derived from a cloned cDNA sequence. An additional cDNA clone that corresponds to about 75% of a similar alpha-type gliadin has been sequenced and shows some important differences. About 97% of the composite sequence of A-gliadin (an al
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7. In Vitro Synthesis of Wheat (Triticum aestivum L.) Storage Proteins 1
Free and membrane-associated polysomes were isolated in approximately equal amounts from endosperm of wheat kernels harvested 20 days after anthesis. The presence of heparin in the homogenizing buffer minimized polysome degradation. Ribonucleic acid from the isolated polysomes, when translated in vitro in a wheat germ system, yielded products ranging in size
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8. Gene Expression in Developing Wheat Endosperm 1: Accumulation of Gliadin and ADPglucose Pyrophosphorylase Messenger RNAs and Polypeptides
The developmental accumulation pattern of messenger RNA transcripts and polypeptides for wheat gliadins and ADPglucose pyrophosphorylase was determined using cDNA and antibody probes. Gliadin mRNA was detected on Northern and RNA dot blots at 3 days after flowering, it increased 100-fold by 10 days and decreased subsequent to 14 days. The abundant mRNAs enco
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9. Analysis of avenin proteins and the expression of their mRNAs in developing oat seeds.
We have isolated and characterized cDNA clones encoding avenins, the prolamine storage proteins of oat seeds. Sequence analysis shows that avenins are a related group of polypeptides and that their mRNAs differ from each other by point mutations and small insertions and deletions. Avenin proteins have structural homology to the alpha/beta-gliadins and gamma-
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10. Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone.
Gliadins, the major wheat seed storage proteins, are encoded by a multigene family. Northern blot analysis shows that gliadin genes are transcribed in endosperm tissue into two classes of poly(A)+ mRNA, 1400 bases (class I) and 1600 bases (class II) in length. Using poly(A)+ RNA from developing wheat endosperm we constructed a cDNA library from which a numbe
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11. The characterization of cDNA clones coding for wheat storage proteins.
Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clo
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12. Investigation of Homologous Crossing over and Sister Chromatid Exchange in the Wheat Nor-B2 Locus Coding for Rrna and Gli-B2 Locus Coding for Gliadins
Recombination was investigated within the Nor-B2 locus of wheat chromosome 6B that contains several thousand of the 18S-5.8S-26S rRNA (rDNA) repeated units. Additionally, recombination was assessed for several chromosome regions, in arm 6Bq between the centromere and the B2 locus (awn suppressor) and in arm 6Bp between the centromere and Nor-B2, between Nor