Glycosidases
Mostrando 1-12 de 145 artigos, teses e dissertações.
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1. Streptozotocin-induced diabetes mellitus affects lysosomal enzymes in rat liver
It has been previously shown that dextran sulfate administered to diabetic rats accumulates in the liver and kidney, and this could be due to a malfunction of the lysosomal digestive pathway. The aim of the present study was to evaluate the expression and activities of lysosomal enzymes that act upon proteins and sulfated polysaccharides in the livers of dia
Braz J Med Biol Res. Publicado em: 2014-06
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2. Inhibition of pancreatic lipase by extracts of Baccharis trimera (Less.) DC., Asteraceae: evaluation of antinutrients and effect on glycosidases
In order to confirm the traditional use of Baccharis trimera (Less.) DC., Asteraceae, for the reduction of weight, plant extracts were evaluated on the activity of pancreatic lipase (PL), an enzyme responsible for hydrolysis of triacylglycerols in the diet for its subsequent absorption. The aqueous and infused extracts did not show inhibitory activity on the
Revista Brasileira de Farmacognosia. Publicado em: 01/04/2011
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3. Characterization of the interdependency between residues that bind the substrate in a β-glycosidase
The manner by which effects of simultaneous mutations combine to change enzymatic activity is not easily predictable because these effects are not always additive in a linear manner. Hence, the characterization of the effects of simultaneous mutations of amino acid residues that bind the substrate can make a significant contribution to the understanding of t
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-01
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4. Molecular bases of the specificity substrate of a β-glicodase / Bases moleculares da especificidade pelo substrato em β-glicosidases
The β-glycosidases of family 1 of the glycoside hydrolases (GH 1) are one of the most important groups of enzymes. These enzymes are involved in a high diversity of physiological functions. The main objective of this study was the analysis of the molecular bases of the specificity for substrate of a β-glycosidase from the larvae of Spodoptera frugi
Publicado em: 2009
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5. Estudo da multiplicidade de formas de ß-glicosidases de Aspergillus versicolor / Study of ß-glycosidases from Aspergillus versicolor
Este trabalho procurou avaliar algumas isozimas envolvidas com o processo de degradação de polissacarídeos da parede celular vegetal, encontradas em Aspergillus versicolor, fungo pertencente a um gênero onde a multiplicidade de componentes enzimáticos com a mesma atividade oscila de uma a três. Duas ß-xilosidases foram purificadas por meio de DEAE-cel
Publicado em: 2008
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6. Antioxidant activity of plants foods. Structure and bioavailability of wild mulberry (Morus sp.) anthocyanins / Atividade antioxidante de alimentos vegetais. Estrutura e estudo de biodisponibilidade de antocianinas de amora silvestre (Morus sp.)
Flavonoids are of great interest in nutrition and medicine because of their potent antioxidant activity and possible protective effects on human health, reducing the risk of chronic diseases such as cardiovascular diseases and cancer. To produce a biological effects in vivo, it is essential that sufficient quantities reach the target tissue. The initial obje
Publicado em: 2005
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7. Purificação, caracterização, clonagem e seqüenciamento de β-glicosidades de Tenebrio molitor (Coleoptera) / Purification, characterization, cloning and sequencing of β-glycosidases from Tenebrio molitor (Coleoptera)
In the midgut lumen of Tenebrio molitor larvae there are 4 β-glycosidases (named 1, 2, 3a and 3b), not present in the animal food. They were purified with electrophoresis, ion exchange and hydrophobic chromatographies. β-glycosidase 1 (relative molecular weight - Mr 59,000) is unstable at 30°C but is stabilized by substrates. The enzyme hydrolyses
Publicado em: 2001
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8. Strategies by which some pathogenic trichomonads integrate diverse signals in the decision-making process
The interaction between each one of Trichomonas vaginalis and Tritrichomonas foetus with their hosts is a complex process in which components associated to the cell surfaces of both parasites and host epithelial cells, and also to soluble components found in vaginal/urethral secretions, are involved. Either cytoadhesion or the cytotoxicity exerted by parasit
Anais da Academia Brasileira de Ciências. Publicado em: 2000-06
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9. Purification and characterization of digestive beta-glycosidases from Spodoptera frugiperda (Lepidoptera) / Purificação e caracterização das β-glicosidases digestivas de Spodoptera frugiperda (Lepidoptera)
Foram purificadas através de uma combinação de cromatografias as duas β- glicosidases digestivas (Mr 47.000 e 50.000 - denominadas β47 e β50, respectivamente) encontradas na larva de S. frugiperda. Experimentos de competição entre substratos e modificação química mostraram que a β47 possui dois sítios ativos. Um desses sítios de
Publicado em: 1999
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10. Obtenção de aza-açucares a partir de D-glucitol
The azasugars (polyhydroxilated pyperidines and pyrrolidines) constitute an important class of natural and synthetic products, due to their ability to inhibit glycosidases, which are enzymes responsible for the cleavage of glycosidic bonds. Inhibition of glycosidases could be of therapeutic value for the treatment of diabetes, cancer, obesity and viral infec
Publicado em: 1996
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11. Formation of glycosidases in batch and continuous culture of Bacteroides fragilis.
Nine strains of bacteroides fragilis were cultivated in stirred fermentors and tested for their ability to produce glycosidases. B. fragilis subsp. vulgatus B70 was used for optimizing the production of glycosidases. The highest bacterial yield was obtained in proteose peptone-yeast extract medium. The optimum pH for maximal bacterial yield was 7.0, and the
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12. Mucin degradation in human colon ecosystems. Isolation and properties of fecal strains that degrade ABH blood group antigens and oligosaccharides from mucin glycoproteins.
We previously reported that the oligosaccharide chains of hog gastric mucin were degraded by unidentified subpopulations numbering approximately 1% of normal human fecal bacteria. Here we report on the enzyme-producing properties of five strains of mucin oligosaccharide chain-degrading bacteria isolated from feces of four healthy subjects. Four were isolated