Green Fluorescence Protein
Mostrando 1-12 de 449 artigos, teses e dissertações.
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1. The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was indu
Int. braz j urol.. Publicado em: 2018-06
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2. Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells
This study aimed to investigate the mechanism of hypoxia-inducible factor-1 alpha (HIF-1α) mediated hypoxia-induced permeability changes in bladder endothelial cells. Models of in vitro hypoxic cell culture of bladder cancer, bladder cancer cells with low HIF-1α expression and HIF-1α RNA interference (RNAi) expression vector were established. Western blot
Braz J Med Biol Res. Publicado em: 18/12/2017
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3. Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics
Abstract Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue in
Braz. J. Microbiol.. Publicado em: 2017-03
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4. Artemisia annua increases resistance to heat and oxidative stresses, but has no effect on lifespan in Caenorhabditis elegans
Abstract It is suggested that oxidative stress induced by cellular reactive oxygen species is one of the major causal factors of aging. The effect of dietary supplementation of anti-oxidants on response to environmental stressors and lifespan has been studied in various model organisms. In the present study, we examine the effect of Artemisia annua extract o
Food Sci. Technol. Publicado em: 31/05/2016
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5. Trypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and
Braz J Med Biol Res. Publicado em: 18/03/2014
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6. Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'
The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. Afte
Brazilian Journal of Microbiology. Publicado em: 2011-03
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7. Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle v
Genetics and Molecular Biology. Publicado em: 19/11/2010
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8. Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease
Objective - To assess the transfection of the gene that encodes green fluorescent protein (GFP) through direct intramyocardial injection. Methods - The pREGFP plasmid vector was used. The EGFP gene was inserted downstream from the constitutive promoter of the Rous sarcoma virus. Five male dogs were used (mean weight 13.5 kg), in which 0.5 mL of saline soluti
Publicado em: 2010
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9. Transformation of Fusarium oxysporum f sp. vasinfectum with GFP and DsRed gene markers. / TRANSFORMAÃÃO DE Fusarium oxysporum f. sp. vasinfectum COM OS GENES MARCADORES GFP e DsRed.
Fusarium oxysporum f sp. vasinfectum, the casual agent of fusarium wilt disease of cotton, is frequent in most cottonâproducing regions in Brazil. The fungus infects roots, colonizes the rhizomes and eventually blocks the vascular system of the pseudostems, leading to the plant collapse. The Green and Red Fluorescent Proteins (GFP and DsRed) emit green and
Publicado em: 2009
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10. Transferência gênica in vivo por meio de eletroporação em músculo sóleo de ratos wistar. / In vivo gene transfer by electroporation in soleus muscle from wistar rats.
The use of electrical pulses for gene transfer has been successfully used to reach significant levels of gene expression in vitro and in vivo. Therefore electroporation can be considered an important device to get further insight on biological mechanisms and also to treatment. The aim of the present work is to achieve a high level of gene transfer throughout
Publicado em: 2008
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11. Interação entre proteínas fluorescentes e nanocristais de CdSe/ZnS / Interaction between fluorescent proteins and CdSe/ZnS nanocrystals
Proteins belonging to the GFP family were used to characterize their interaction with fluorescent CdSe/ZnS nanocrystals and to verify the occurrence of resonance energy transfer (FRET) among these elements. Two donor-acceptor pairs were established, one having a protein as donor and the other having a nanocrystal as donor. In both cases the donor suffers que
Publicado em: 2007
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12. Evaluation of effectiveness of the sanitization of a water purification system. Sterilization of medical devices, residual dissipation of ethylene oxide and the use of green fluorescent protein (GFP) as an indicator of process control / Avaliação de eficácia da sanitização de um sistema de purificação de água. Esterilização de artigos médicos, dissipação residual do óxido de etileno e uso da proteína verde fluorescente (GFP) como inidicador de controle do processo
The water exerts important paper in different phases of critical items manufacture in the health care units, pharmaceutical industries, hospitals and clinics, becoming necessary a rigorous control of the water purification systems, storage and distribution, in order to prevent biofilms formation and cross-contamination between devices and patients, who are s
Publicado em: 2007