Growth Pseudohyphal
Mostrando 1-12 de 72 artigos, teses e dissertações.
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1. Characteristics of Saccharomyces cerevisiae yeasts exhibiting rough colonies and pseudohyphal morphology with respect to alcoholic fermentation
Among the native yeasts found in alcoholic fermentation, rough colonies associated with pseudohyphal morphology belonging to the species Saccharomyces cerevisiae are very common and undesirable during the process. The aim of this work was to perform morphological and physiological characterisations of S. cerevisiae strains that exhibited rough and smooth col
Braz. J. Microbiol.. Publicado em: 04/03/2014
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2. Candida albicans morphologies revealed by scanning electron microscopy analysis
Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/ or CPH1 (strains HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans' morphol
Braz. J. Microbiol.. Publicado em: 10/12/2013
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3. Caracterização de linhagens industriais de Saccharomyces cerevisiae quanto a filamentação induzida por álcoois e deficiência de nutrientes / Characterization of industrial strains of Saccharomyces cerevisiae by filamentous growth induced by alcohol and nutrient deprivation
O uso de microrganismos na biotecnologia tem grande importância e interesse econômico no Brasil. Entre esses microrganismos a levedura Saccharomyces cerevisiae tem grande destaque nos processos fermentativos para produção de pães, bebidas e álcool combustível. Dimorfismo em S. cerevisiae (alteração na morfologia celular de células brotantes para es
Publicado em: 2006
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4. Estudo da atividade de drogas antifungicas atraves de curvas de crescimento de Candida albicans utilizando o sistema automatizado Bio-Cell Tracer
Candida species are opportunistic fungal pathogens that are often the causative agents of infections in immunocompromised patients. amphotericin B (AMPH), itraconazole (ITCZ), fluconazole (FLCZ) and 5-flucytosine (5-FC) are the principal drugs used on the treatment of candidemic patients. However, in some cases, the clinical responses of Candida are differen
Publicado em: 2003
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5. Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development.
When starved for nitrogen, MATa/MAT alpha cells of the budding yeast Saccharomyces cerevisiae undergo a dimorphic transition to pseudohyphal growth. A visual genetic screen, called PHD (pseudohyphal determinant), for S. cerevisiae pseudohyphal growth mutants was developed. The PHD screen was used to identify seven S. cerevisiae genes that when overexpressed
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6. Isolation and Characterization of EPD1, an Essential Gene for Pseudohyphal Growth of a Dimorphic Yeast, Candida maltosa
Additional copies of the centromeric DNA (CEN) region induce pseudohyphal growth in a dimorphic yeast, Candida maltosa (T. Nakazawa, T. Motoyama, H. Horiuchi, A. Ohta, and M. Takagi, J. Bacteriol. 179:5030–5036, 1997). To understand the mechanism of this transition, we screened the gene library of C. maltosa for sequences which could suppress this morpholo
American Society for Microbiology.
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7. The Saccharomyces cerevisiae mutation elm4-1 facilitates pseudohyphal differentiation and interacts with a deficiency in phosphoribosylpyrophosphate synthase activity to cause constitutive pseudohyphal growth.
Saccharomyces cerevisiae mutant E124 was selected in a visual screen based on elongated cell shape. Genetic analysis showed that E124 contains two separate mutations, pps1-1 and elm4-1, each causing a distinct phenotype inherited as a single-gene trait. In rich medium, pps1-1 by itself causes increased doubling time but does not affect cell shape, whereas el
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8. Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast.
Pseudohyphal differentiation in Saccharomyces cerevisiae was first described as a response of diploid cells to nitrogen limitation. Here we report that haploid and diploid starch-degrading S. cerevisiae strains were able to switch from a yeast form to a filamentous pseudohyphal form in response to carbon limitation in the presence of an ample supply of nitro
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9. Different Domains of the Essential GTPase Cdc42p Required for Growth and Development of Saccharomyces cerevisiae
In budding yeast, the Rho-type GTPase Cdc42p is essential for cell division and regulates pseudohyphal development and invasive growth. Here, we isolated novel Cdc42p mutant proteins with single-amino-acid substitutions that are sufficient to uncouple functions of Cdc42p essential for cell division from regulatory functions required for pseudohyphal developm
American Society for Microbiology.
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10. Evidence that part of a centromeric DNA region induces pseudohyphal growth in a dimorphic yeast, Candida maltosa.
We observed that a YCp-type vector having the centromeric DNA (CEN) sequence previously isolated from the genome, but not a YRp-type vector lacking the CEN sequence, induced pseudohyphal growth in a dimorphic fungi, Candida maltosa, which had been shown to be closely related to Candida albicans by phylogenetic analysis. Deletion analysis of the CEN sequence
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11. The three yeast A kinases have specific signaling functions in pseudohyphal growth
The three yeast A kinase catalytic subunit isoforms are redundant for viability. We demonstrate that they have dramatically different roles in pseudohyphal development: Tpk2 is essential, whereas Tpk3 inhibits. Tpk1 has no discernible effect. Two-hybrid analysis identified the transcription factor Sfl1 as a protein that interacts specifically with Tpk2, but
The National Academy of Sciences.
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12. Ash1, a Daughter Cell-Specific Protein, Is Required for Pseudohyphal Growth of Saccharomyces cerevisiae
Ash1 (for asymmetric synthesis of HO) was first uncovered in genetic screens that revealed its role in mating-type switching. Ash1 prevents HO expression in daughter cells. Because Ash1 has a zinc finger-like domain related to that of the GATA family of transcription factors, it presumably acts by repressing HO transcription. Nonswitching diploid cells also
American Society for Microbiology.