Heparinase
Mostrando 1-12 de 84 artigos, teses e dissertações.
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1. Carlos Américo Paiva Gonçalves Filho
RESUMO Analisamos as amostras das cápsulas anteriores do cristalino de uma paciente com esclerose sistêmica e comparamos com as de um paciente controle. Não foram observadas diferenças significativas entre esclerose sistêmica e controle nos resultados da coloração com hematoxilina-eosina e picrosirius. Nas amostras obtidas da esclerose sistêmica e do
Arq. Bras. Oftalmol.. Publicado em: 2021-06
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2. Relato sobre estudo morfológico e molecular da cápsula anterior do cristalino na esclerose sistêmica
RESUMO Analisamos as amostras das cápsulas anteriores do cristalino de uma paciente com esclerose sistêmica e comparamos com as de um paciente controle. Não foram observadas diferenças significativas entre esclerose sistêmica e controle nos resultados da coloração com hematoxilina-eosina e picrosirius. Nas amostras obtidas da esclerose sistêmica e do
Arq. Bras. Oftalmol.. Publicado em: 2021-06
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3. Heparinases: Clonagem, Expressão e Requisitos Estruturais para a Atividade. / Heparinases: Cloning, Expression and Structural Requirements for Activity.
Características estruturais de heparina (Hep) e heparam sulfato (HS) têm sido determinadas usando enzima de Flavobacterium heparinum, uma bactéria de solo, não-patogênica. Sob indução com Hep/HS ou seus dissacarídeos como única fonte de carbono e nitrogênio, a bactéria sintetiza heparinase e heparitinases I e II. Essas liases clivam heparina e HS
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/04/2011
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4. Caracterização molecular da enzima heparinase II de flavobacterium heparinum através de simulações de dinâmica molecular
A hemostasia envolve diversos eventos fisiológicos desencadeados após a ruptura da integridade vascular. Durante estes processos, os glicosaminoglicanos, incluindo a heparina e o heparan sulfato, desempenham funções fisiológicas fundamentais. Particularmente a heparina, isolada no início do século XX, permanece até os dias atuais como um dos mais efi
Publicado em: 2009
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5. Specific plate assay for bacterial heparinase.
A procedure was developed for detecting heparinase activity on heparin agar plates. The method is based on the differential precipitation of heparin and heparinase-generated heparin fragments by protamine sulfate. Heparinase activity is detected by the presence of clear zones against a white background. This method can be used to screen for the expression of
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6. Heparinase production by Flavobacterium heparinum.
Heparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold over that achieved by previously published methods to 375 U/liter per h in the complex medium. Rapid deactivation of heparinase activity, bo
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7. Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum.
Upon induction with heparin, Flavobacterium heparinum synthesizes and secretes into its periplasmic space heparinase I (EC 4.2.2.7), heparinase II, and heparinase III (heparitinase; EC 4.2.2.8). Heparinase I degrades heparin, and heparinase II degrades both heparin and heparan sulfate, while heparinase III degrades heparan sulfate predominantly. We isolated
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8. Direct evidence for a predominantly exolytic processive mechanism for depolymerization of heparin-like glycosaminoglycans by heparinase I
Heparinase I from Flavobacterium heparinum has important uses for elucidating the complex sequence heterogeneity of heparin-like glycosaminoglycans (HLGAGs). Understanding the biological function of HLGAGs has been impaired by the limited methods for analysis of pure or mixed oligosaccharide fragments. Here, we use methodologies involving MS and capillary el
The National Academy of Sciences.
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9. Sulfur regulation of heparinase and sulfatases in Flavobacterium heparinum.
Sulfur regulation of heparinase synthesis and sulfatase synthesis was studied in Flavobacterium heparinum. Heparinase synthesis was strongly repressed by sulfate and L-cysteine, while the activity of this enzyme showed little or no inhibition by these compounds. Heparinase was synthesized in the absence of heparin when L-methionine was used as the sole sulfu
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10. Cloning and expression of heparinase I gene from Flavobacterium heparinum.
Heparinases, enzymes that cleave heparin and heparin sulfate, are implicated in physiological and pathological functions ranging from wound healing to tumor metastasis and are useful in deheparinization therapies. We report the cloning of the heparinase I (EC 4.2.2.7) gene from Flavobacterium heparinum using PCR. Two degenerate oligonucleotides, based on the
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11. Mass spectrometric evidence for the enzymatic mechanism of the depolymerization of heparin-like glycosaminoglycans by heparinase II
Heparin-like glycosaminoglycans, acidic complex polysaccharides present on cell surfaces and in the extracellular matrix, regulate important physiological processes such as anticoagulation and angiogenesis. Heparin-like glycosaminoglycan degrading enzymes or heparinases are powerful tools that have enabled the elucidation of important biological properties o
The National Academy of Sciences.
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12. Expression System for High Levels of GAG Lyase Gene Expression and Study of the hepA Upstream Region in Flavobacterium heparinum
A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chromosome to yield stable transconjugant str
American Society for Microbiology.