High Affinity Antibodies
Mostrando 1-12 de 523 artigos, teses e dissertações.
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1. Development of an indirect ELISA assay to evaluation of the adaptive immune response of pacu (Piaractus mesopotamicus)
ABSTRACT The pacu is one of the most important species for Brazilian fish farming and is considered emerging in the global aquaculture. Despite its importance, no effective tool for evaluation of the adaptive immune response of this species has been developed. Therefore, this study aimed the development and standardization of indirect ELISA for the measureme
An. Acad. Bras. Ciênc.. Publicado em: 25/06/2018
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2. Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Abstract Background The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 06/07/2017
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3. Aptamers as a promising approach for the control of parasitic diseases
ABSTRACT Aptamers are short single-stranded RNA or DNA oligonucleotides that are capable of binding various biological targets with high affinity and specificity. Their identification initially relies on a molecular process named SELEX (Systematic Evolution of Ligands by EXponential enrichment) that has been later modified in order to improve aptamer sensiti
Braz J Infect Dis. Publicado em: 2016-12
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4. Produção e caracterização da porção Fab do anticorpo anti-digoxina utilizando a tecnologia de phage display. / Production and characterization of the Fab portion of anti-digoxin antibody by phage display technology.
Digoxin is a pharmaceutical used in the control of cardiac dysfunction. Its therapeutic window is very narrow. To counteract the toxic effect, polyclonal anti-digoxin Fab fragments are commercially available. Our goal was to obtain variants of monoclonal anti-digoxin Fab fragments by phage display technology, which allows the generation of high affinity and
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/03/2012
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5. Papel das citocinas IL-5 e IL-17A na diferenciação de células produtoras de anticorpos de vida longa (ASC) induzida pelo veneno do peixe Thalassophryne nattereri / The role of IL-5 and IL-17A in the differentiation of long-lived antibody secreting cells (ASC) induced by Thalassophryne nattereri fish venom
O veneno do T.nattereri induz uma resposta de memória com a diferenciação de células B B220neg, indicativo de células produtoras de anticorpos de vida longa (ASC). Para avaliar o efeito do veneno na diferenciação de ASCs, camundongos BALB/c foram imunizados e sacrificados nos dias 21, 28, 48, 74 e 120 para avaliação de anticorpos plasmáticos e cél
Publicado em: 2009
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6. Estudo longitudinal de marcadores sorológicos para o imunodiagnóstico da toxoplasmose aguda humana.
Toxoplasma gondii is thought to infect one third of the world population. Acute infection is often asymptomatic, but if acquired during pregnancy, T. gondii can be transmitted vertically, through the placenta, from mother to fetus, who is at high risk of developing severe disease. The commercially available serological tests have several limitations such as
Publicado em: 2009
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7. Basophils activation profile and evidence of histamine release factor in Chronic Idiopathic Urticaria / Perfil de ativação de basófilos e evidência de fatores liberadores de histamina na urticária crônica idiopática
INTRODUCTION: Chronic Urticaria is characterized by recurrent, transitory, pruritic and erythematous wheals present for at least six weeks. In most patients the cause is unknown, defined as idiopathic (CIU), however, a sub-group has autoantibodies against the alfa chain of the high affinity IgE receptor (FceRIa) expressed on mast cells and basophils surface
Publicado em: 2009
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8. Caracterização estrutural da forma recombinante da proteína Pb27 de Paracoccidioides brasiliensis
COITINHO, J. B. Structural characterization of recombinant form of the protein Pb27 from Paracoccidioides brasiliensis. 2009. Dissertation (Masters). Departamento de Bioquímica e Imunologia. Universidade Federal de Minas Gerais. Belo Horizonte, 2009. Paracoccidiodomycosis (PCM) is one of the most important systemic mycoses in Brazil. The etiologic agent of
Publicado em: 2009
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9. Detecção do vírus da leprose dos citros nos tecidos da planta infectada e do ácaro vetor Brevipalpus phoenicis Geijskes (Acari: Tenuipalpidae) / Detection of Citrus leprosis virus C (CiLV-C) in the tissues of infected plants and of mite vector Brevipalpus phoenicis Geijskes (Acari: Tenuipalpidae)
Citrus leprosis is one of the most important diseases in the Brazilian citrus production due to the wide occurrence in orchards and also to the high costs involved in the chemical control of the mite vector. The disease affects the plant production and longevity and it is characterized by localized chlorotic and/or necrotic spots on the leaves, stems and fru
Publicado em: 2009
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10. Improving the production of the denatured recombinant N-terminal domain of rhoptry-associated protein 2 from a Plasmodium falciparum target in the pathology of anemia in falciparum malaria
Rhoptry-associated protein 2 (RAP2) is known to be discharged from rhoptry onto the membrane surface of infected and uninfected erythrocytes (UEs) ex vivo and in vitro and this information provides new insights into the understanding of the pathology of severe anemia in falciparum malaria. In this study, a hexahistidine-tagged recombinant protein correspondi
Memórias do Instituto Oswaldo Cruz. Publicado em: 2008-09
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11. Estudo da imunogenicidade da proteína de classe 3 (PorB) purificada da membrana externa de Neisseria miningitidis: imunização intranasal/intramuscular em camundongos adultos e neonatos utilizando Bordetella pertussis como adjuvante. / Study of the immunogenecity of the class 3 proteins (PorB) purified from the outer mebrane of Neisseria meningitidis: intranasal and intramuscular immunization in adult and neonate mice using Bordetella pertussis as adjuvant.
Proteins of class 3 sound candidates in the preparation of vaccine against meningococcal illness. The aim of this study was to determine the immunogenicity of class 3 proteins purified of Neisseria meningitidis of the serogroup B along with whole cells of Bordetella pertussis as adjuvant. BALB/c and outbred neonate mice between 3 and 12 days old were immuniz
Publicado em: 2008
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12. ConstruÃÃo de uma biblioteca de anticorpos (scFv) apresentada em fagos para seleÃÃo, anÃlise e caracterizaÃÃo de antÃgenos do carrapato bovino (Boophilus microplus)
CAPÃTULO I: The amplification of fragmentos scFv (single chain fragments variable) of immunized animals is an important method to generation of monoclonal antibodies. In this work chickens had been immunized with proteins of the larval and adult phases of the Boophilus microplus for the generation of an antibodies combinatorial library. Total RNA of spleen
Publicado em: 2007