Histatins
Mostrando 1-12 de 17 artigos, teses e dissertações.
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1. Standardization of a protocol for shotgun proteomic analysis of saliva
Abstract Saliva contains numerous proteins and peptides, each of them carries a number of biological functions that are very important in maintaining the oral cavity health and also yields information about both local and systemic diseases. Currently, proteomic analysis is the basis for large-scale identification of these proteins and discovery of new biomar
J. Appl. Oral Sci.. Publicado em: 11/06/2018
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2. Comparison of histatin levels between periodontally healthy and diseased subjects / Comparação dos níveis de histatinas da saliva da parótida entre sujeitos com doença periodontal e sujeitos sem história prévia de doença periodontal
As histatinas são uma família de peptídeos presentes nas secreções salivares humanas tendo como membros mais importantes da família as histatinas 1, 3 e 5. Diversos trabalhos demonstraram atividade inibitória das histatinas sobre produtos bacterianos e derivados do hospedeiro envolvidos na doença periodontal, sugerindo uma função importante de defe
Publicado em: 2005
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3. Anticandidal activity of major human salivary histatins.
We have previously shown that histatins 1, 3, and 5 are homologous, histidine-rich proteins present in human parotid and submandibular secretions which contain 38, 32, and 24 amino acids, respectively. Interest in these proteins stems from the fact that histatins exhibit candidacidal and candidastatic activities. The goal of the present investigation was a d
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4. Inhibitory effects of human salivary histatins and lysozyme on coaggregation between Porphyromonas gingivalis and Streptococcus mitis.
The effects of histatins on coaggregation between Porphyromonas gingivalis 381 and Streptococcus mitis ATCC 9811 were investigated by using a turbidimetric assay. The coaggregation activity was significantly inhibited by histatins 5 and 8 and strongly by lysozyme. Tritium-labeled histatin 8 bound to P. gingivalis cells but not to S. mitis cells.
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5. Candidacidal activity of recombinant human salivary histatin-5 and variants.
Human salivary histatins possess fungicidal and bactericidal activities. The current investigation evaluates the structure-function relationship of histatins with regard to their candidacidal activity by using recombinant histatin-5 and its variants produced in Escherichia coli. The purified recombinant histatins were examined for their candidacidal activity
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6. Kinetics of histatin proteolysis in whole saliva and the effect on bioactive domains with metal-binding, antifungal, and wound-healing properties
The present study was undertaken to investigate the rate and mode of degradation of individual histatin proteins in whole saliva to establish the impact on its functional domains. Pure synthetic histatins 1, 3, and 5 were incubated with whole saliva supernatant as the enzyme source, and peptides in the resultant digests were separated by reverse-phase-HPLC a
The Federation of American Societies for Experimental Biology.
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7. Cooperation of Salivary Protein Histatin 3 with Heat Shock Cognate Protein 70 Relative to the G1/S Transition in Human Gingival Fibroblasts*
Histatins, a family of salivary proteins, have antimicrobial activity. Candida albicans, which is killed by histatins, induces oral candidiasis in individuals with compromised immune systems. Although the functional significance of histatins has been documented, their biological and physiological functions against host cells have not been clarified. In t
American Society for Biochemistry and Molecular Biology.
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8. Histatin 3-Mediated Killing of Candida albicans: Effect of Extracellular Salt Concentration on Binding and Internalization
Human saliva contains histidine-rich proteins, histatins, which have antifungal activity in vitro. The mechanism by which histatins are able to kill Candida albicans may have clinical significance but is currently unknown. Using radiolabeled histatin 3, we show that the protein binds to C. albicans spheroplasts in a manner that is dependent on time and conce
American Society for Microbiology.
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9. Studies of the mechanism of human salivary histatin-5 candidacidal activity with histatin-5 variants and azole-sensitive and -resistant Candida species.
Histatins are a group of small, cationic, antifungal peptides present in human saliva. A previous molecular modeling analysis suggested structural similarity between the Phe14-His15 and His18-His19 dipeptide sequences in histatin-5 (Hsn-5; a 24-amino-acid polypeptide) and the sequence of miconazole (one of the azole-based antifungal therapeutic agents), impl
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10. Dialysis Unmasks the Fungicidal Properties of Glandular Salivary Secretions
Several salivary proteins exhibit fungicidal activity against the opportunistic oral pathogen Candida albicans when they are tested as pure proteins in vitro. However, salivary secretions that are examined by the same assays either lack or exhibit very low candidacidal activity. Since ionic strength is known to have an inhibitory effect on the fungicidal act
American Society for Microbiology.
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11. Salivary Histatin 5 and Human Neutrophil Defensin 1 Kill Candida albicans via Shared Pathways
Salivary histatins are a family of basic histidine-rich proteins in which therapeutic potential as drugs against oral candidiasis is apparent, considering their potent in vitro antifungal activity and lack of toxicity to humans. Histatin 5 (Hst 5) kills the fungal pathogen Candida albicans via a mechanism that involves binding to specific sites on the yeast
American Society for Microbiology.
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12. Released ATP Is an Extracellular Cytotoxic Mediator in Salivary Histatin 5-Induced Killing of Candida albicans
Salivary histatins (Hsts) are antifungal peptides with promise as therapeutic agents against candidiasis. Hst 5 kills the fungal pathogen Candida albicans via a mechanism that involves release of cellular ATP in the absence of cytolysis. Here we demonstrate that released ATP has a further role in Hst 5 killing. Incubation of the cells with ATP analogues indu
American Society for Microbiology.