The adherence of [3H]thymidine-labeled Streptococcus sanguis strains to bare hydroxyapatite and to hydroxyapatite coated with a range of concentrations of lysozyme, poly-L-lysine, poly-L-glutamic acid, whole saliva supernatant, and combinations of some of the above was studied. A...
Ver documentoTransmission electron microscopy and standard chemiluminescence assays were used to investigate the in vivo effect of dichloromethylene bisphosphonate (clodronate) on the phagocytosis of pure hydroxyapatite particles by rat peritoneal macrophages and the production of chemilumine...
Ver documentoA method of synovial fluid preparation giving optimal hydroxyapatite detection as well as definitions of the threshold masses of hydroxyapatite in viscous synovial fluid detectable by x ray diffraction and scanning electron microscopy with energy dispersive analysis is reported. ...
Ver documentoCalcium hydroxyapatite can be a significant component of black pigment gallstones. Diverse molecules that bind calcium phosphate inhibit hydroxyapatite precipitation. Because glycine-conjugated bile acids, but not their taurine counterparts, bind calcium phosphate, we studied whe...
Ver documentoBiomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapat...
Ver documentoA Fusarium dextranase and a Penicillium dextranase were compared for their relative ability to quantitatively reduce the adsorption of 3H-labeled Steptococcus mutans cells onto hydroxyapatite. Fusarium dextranase-treated hydroxyapatite disks caused a statistically significant dec...
Ver documentoSpecific antibodies and their Fab fragments were used to study adsorption of Antinomyces viscosus T14V to saliva-treated hydroxyapatite. Antibody against A. viscosus T14V blocked adsorption of the strain to saliva-treated hydroxyapatite, and this activity was removed after preinc...
Ver documentoDenatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced th...
Ver documentoHydroxyapatite crystals have been found in synovial fluids and periarticular spaces in association with osteoarthritis. This study examined the morphology of the interaction of macrophages and synovial cells with hydroxyapatite in order to determine the fate of hydroxyapatite ove...
Ver documentoThe sol-gel process is a technique used to synthesize materials from colloidal suspensions and, therefore, is suitable for preparing materials in the nanoscale. In this work hydroxyapatite was used due to its known properties in tissue engineering. Hydroxyapatite Ca10(PO4)6(OH)2 ...
Ver documentoDestructive osteoarthritis is characterised by rapidly progressive joint destruction associated with intra-articular deposition of hydroxyapatite crystals. The possible role of such crystals in the pathogenesis of this condition was investigated by testing the ability of hydroxya...
Ver documentoThe adherence of Streptococcus mutans PK1 to hydroxyapatite disks pretreated with various acidic and basic proteins in imidazole buffer was studied. Adsorption of a basic protein onto an hydroxyapatite disk enhanced or had no effect on bacterial adherence, whereas adsorption of a...
Ver documentoBone sialoprotein (BSP) and osteopontin, the major phosphorylated proteins of mammalian bone, have been proposed to function in the initiation of mineralization. To test this hypothesis, the effects of BSP and osteopontin on hydroxyapatite crystal formation were determined by usi...
Ver documentoThe influence of growth in the presence of sublethal concentrations of nine antibiotics on the ability of certain potentially odontopathic bacteria to attach to saliva-treated hydroxyapatite surfaces which mimic teeth was studied. Cells of Actinomyces viscosus LY7 and S2, Bactero...
Ver documentoThe biocompatibility of commercially pure (cp) titanium stems from its chemical stability within an organism, due to a fine film of impermeable titanium oxide covering the metal surface, which guarantees its resistance to corrosion. Despite its biocompatible characteristic, this ...
Ver documentoHydroxyapatite crystals were incubated with human polymorphonuclear leucocytes and samples examined by light and electron microscopy after 3, 8, 30, and 120 minutes' incubation. Phagocytosis of crystals occurred at 3 minutes and increased with incubation. Degranulation of neutrop...
Ver documentoExopolysaccharide produced by a microorganism Zoogloea sp. obtained from fermentation of molasses sugarcane is a promising material for biomedical and pharmaceutical uses with low toxicity and biocompatibility established. The present work is comprise the biopolymer of sugar cane...
Ver documentoIn the present study, mechanical properties of porous glass reinforced hydroxyapatite bioceramics were assessed by microhardness, bending and compression tests and fracture toughness determination. Porous discs were produced by a dry method using wax spheres as pore formers. Gree...
Ver documentoWe compared the binding of Streptococcus salivarius HB and the mutant HB-7 to oral surfaces in vivo. Mutant HB-7 does not aggregate with saliva nor does it bind to buccal epithelium, but it does retain its ability to coaggregate with Veillonella and Fusobacterium. At 1 h after in...
Ver documentoAdsorption of all of eight strains of Streptococcus mutans to saliva-treated hydroxyapatite (S-HA) surfaces was inhibited by galactose and melibiose, but not by other neutral sugars tested. This observation supports the hypothesis that lectin-like components participate in the at...
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