Ide8 Cells
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Transmitted human immunodeficiency virus-1 drug resistance in a cohort of men who have sex with men in Belo Horizonte, Brazil - 1996-2012
The presence of transmitted human immunodeficiency virus (HIV)-1 drug-resistance (TDR) at the time of antiretroviral therapy initiation is associated with failure to achieve viral load (VL) suppression. Here, we report TDR surveillance in a specific population of men who have sex with men (MSM) in Belo Horizonte, Brazil. In this study, the rate of TDR was ev
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-06
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2. Estabelecimento e propagação in vitro de uma amostra brasileira de Anaplasma marginale com apêndice em células IDE8 (Ixodes scapularis)
Uma amostra brasileira de Anaplasma marginale com apêndice foi estabelecida e mantida in vitro em uma linhagem de células de carrapatos (IDE8). A infecção foi confirmada através de microscopia ótica e eletrônica de transmissão. Além disso, os primers MSP1aNF2 e MSP1aNR2 amplificaram produtos do DNA extraído das células infectadas. Comparações de
Brazilian Journal of Microbiology. Publicado em: 2009-06
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3. Manutenção in vitro de células IDE8 em dois tipos de soro bovino
The present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine ser
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2007-04
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4. Growth of Cowdria ruminantium, the Causative Agent of Heartwater, in a Tick Cell Line
The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
American Society for Microbiology.
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5. Isolation of the equine granulocytic ehrlichiosis agent, Ehrlichia equi, in tick cell culture.
The equine granulocytic ehrlichiosis agent, Ehrlichia equi, is closely related or identical to the human granulocytic ehrlichiosis (HGE) agent. Both are suspected of being transmitted by ticks. We have successfully isolated E. equi in a cell line, IDE8, derived from a putative vector, the tick Ixodes scapularis. Peripheral blood leukocytes from an experiment